Cucumber leaf spot virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Aureusvirus

Average proteome isoelectric point is 7.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8CZ23|A8CZ23_9TOMB p17 OS=Cucumber leaf spot virus OX=165432 PE=4 SV=1
MM1 pKa = 7.86EE2 pKa = 4.39NTQGGVLCPNRR13 pKa = 11.84CKK15 pKa = 10.67VCSHH19 pKa = 5.91TTCIRR24 pKa = 11.84EE25 pKa = 3.96SSGQGGRR32 pKa = 11.84QASRR36 pKa = 11.84FTRR39 pKa = 11.84LITEE43 pKa = 3.8PRR45 pKa = 11.84IVSEE49 pKa = 3.89QGIQYY54 pKa = 7.55RR55 pKa = 11.84TWIADD60 pKa = 3.32RR61 pKa = 11.84GFPIALLSSSGGLSTSIRR79 pKa = 11.84GHH81 pKa = 5.29GVAVTVQGDD90 pKa = 4.09SKK92 pKa = 11.61SLLNFCRR99 pKa = 11.84VAYY102 pKa = 10.11DD103 pKa = 3.52VFNHH107 pKa = 6.02PVIQSEE113 pKa = 4.62VCYY116 pKa = 10.72GRR118 pKa = 11.84GPSTSDD124 pKa = 2.86EE125 pKa = 4.07VRR127 pKa = 11.84AEE129 pKa = 4.13FQDD132 pKa = 3.4EE133 pKa = 3.98AGIIQGRR140 pKa = 11.84LPQSEE145 pKa = 4.32TEE147 pKa = 4.39EE148 pKa = 4.1NNKK151 pKa = 9.94AA152 pKa = 3.31

Molecular weight: 16.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8CZ21|A8CZ21_9TOMB p27 OS=Cucumber leaf spot virus OX=165432 PE=3 SV=1
MM1 pKa = 7.66EE2 pKa = 5.1IARR5 pKa = 11.84TNKK8 pKa = 9.71NSVVKK13 pKa = 8.68YY14 pKa = 9.71VPAAVGAAYY23 pKa = 10.58QMGKK27 pKa = 10.27SIVPYY32 pKa = 10.54APTIVDD38 pKa = 3.15ALGNVVSRR46 pKa = 11.84ATGRR50 pKa = 11.84KK51 pKa = 8.67KK52 pKa = 10.16KK53 pKa = 10.74SKK55 pKa = 10.31GKK57 pKa = 9.45EE58 pKa = 3.73VQNQIVGGIGAIAAPVSITKK78 pKa = 10.12RR79 pKa = 11.84VRR81 pKa = 11.84GMRR84 pKa = 11.84PSFRR88 pKa = 11.84QTKK91 pKa = 8.35GKK93 pKa = 8.67VHH95 pKa = 5.99IVHH98 pKa = 6.85RR99 pKa = 11.84EE100 pKa = 3.74LVTSVINLVGNFRR113 pKa = 11.84VNNNVSAQIGQFRR126 pKa = 11.84INPSNSSLFTWLPTIASNFDD146 pKa = 3.38SYY148 pKa = 11.49RR149 pKa = 11.84FTSIRR154 pKa = 11.84FVYY157 pKa = 10.57VPLCATTEE165 pKa = 4.01TGRR168 pKa = 11.84VSLFWDD174 pKa = 4.23KK175 pKa = 11.29DD176 pKa = 3.79SQDD179 pKa = 3.91PLPVDD184 pKa = 4.13RR185 pKa = 11.84AALSSYY191 pKa = 9.48GHH193 pKa = 6.23SNEE196 pKa = 3.9GPPWAEE202 pKa = 3.7TTLNVPTDD210 pKa = 3.55GKK212 pKa = 10.76QRR214 pKa = 11.84FVTDD218 pKa = 3.13SNTTDD223 pKa = 3.11RR224 pKa = 11.84KK225 pKa = 10.55LVDD228 pKa = 3.99LGQFAFATYY237 pKa = 10.74AGGSNNQIGDD247 pKa = 3.43IYY249 pKa = 11.18VEE251 pKa = 4.09YY252 pKa = 10.57GVEE255 pKa = 4.13FSEE258 pKa = 4.33AQPAGGLTQYY268 pKa = 8.43ITKK271 pKa = 10.37SVGATASTTGPSYY284 pKa = 11.42VVDD287 pKa = 3.87ANINVNATTANVEE300 pKa = 4.27FFSPGTFLITAVVYY314 pKa = 10.53GSTIASPSMAGGNGTLIGDD333 pKa = 4.35LPVVGGSNASIWTCVFSTTGVSTSVPTFTQAGTGLTRR370 pKa = 11.84VQYY373 pKa = 10.32TITRR377 pKa = 11.84VNSQTAYY384 pKa = 10.23QVV386 pKa = 2.96

Molecular weight: 41.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1741

152

738

348.2

38.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.203 ± 0.697

2.585 ± 0.726

4.25 ± 0.409

5.686 ± 0.885

3.619 ± 0.226

7.41 ± 0.768

1.666 ± 0.278

5.342 ± 0.522

5.342 ± 0.682

9.133 ± 1.569

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.757 ± 0.68

3.331 ± 0.862

4.71 ± 0.175

3.389 ± 0.717

6.433 ± 0.669

8.099 ± 0.826

5.744 ± 1.442

9.075 ± 0.606

1.321 ± 0.164

3.848 ± 0.463
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski