Ralstonia phage 1 NP-2014

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6CLR8|W6CLR8_9VIRU Termination of virion assembly protein OS=Ralstonia phage 1 NP-2014 OX=1460070 PE=4 SV=1
MM1 pKa = 7.52GPFSASCNDD10 pKa = 3.48DD11 pKa = 3.58FTVLGAWLTIAKK23 pKa = 9.83ALAVLGGGLAMFVVLAVVLIVCGRR47 pKa = 11.84YY48 pKa = 10.02LFMFADD54 pKa = 4.29RR55 pKa = 11.84VMGWGEE61 pKa = 3.75EE62 pKa = 4.06

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6CWV3|W6CWV3_9VIRU GRPE-like protein OS=Ralstonia phage 1 NP-2014 OX=1460070 PE=4 SV=1
MM1 pKa = 7.68LYY3 pKa = 10.66GYY5 pKa = 10.95ARR7 pKa = 11.84VSTQEE12 pKa = 4.02QEE14 pKa = 3.86THH16 pKa = 5.91AQTDD20 pKa = 3.41ALFRR24 pKa = 11.84AGVGFIFSEE33 pKa = 4.23KK34 pKa = 9.61RR35 pKa = 11.84SARR38 pKa = 11.84STVGRR43 pKa = 11.84PQLEE47 pKa = 4.03KK48 pKa = 10.91LLRR51 pKa = 11.84TLKK54 pKa = 10.72PGDD57 pKa = 3.37QVIVYY62 pKa = 9.69KK63 pKa = 10.45LDD65 pKa = 4.52RR66 pKa = 11.84IARR69 pKa = 11.84SLKK72 pKa = 9.78DD73 pKa = 3.24LLRR76 pKa = 11.84IIEE79 pKa = 4.5RR80 pKa = 11.84IEE82 pKa = 3.66EE83 pKa = 3.85KK84 pKa = 10.54GAQFRR89 pKa = 11.84SITEE93 pKa = 4.2SLDD96 pKa = 3.16TTTPAGRR103 pKa = 11.84MLFHH107 pKa = 6.47MVGAFAEE114 pKa = 4.73FEE116 pKa = 4.09RR117 pKa = 11.84EE118 pKa = 4.26LIRR121 pKa = 11.84EE122 pKa = 3.93RR123 pKa = 11.84TIAGMEE129 pKa = 3.72AAVRR133 pKa = 11.84RR134 pKa = 11.84GVKK137 pKa = 10.29LGRR140 pKa = 11.84HH141 pKa = 4.72YY142 pKa = 11.68AMSRR146 pKa = 11.84EE147 pKa = 4.25DD148 pKa = 3.3EE149 pKa = 4.47AEE151 pKa = 4.13ALRR154 pKa = 11.84LWHH157 pKa = 6.32QGTMTKK163 pKa = 10.39SAIARR168 pKa = 11.84MYY170 pKa = 8.75GVHH173 pKa = 5.92MSSIKK178 pKa = 10.22RR179 pKa = 11.84AIKK182 pKa = 9.65RR183 pKa = 11.84HH184 pKa = 4.66QEE186 pKa = 3.92SVQPSLLDD194 pKa = 3.45AAA196 pKa = 5.04

Molecular weight: 22.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16

0

16

2343

28

506

146.4

16.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.993 ± 1.109

1.835 ± 0.372

5.25 ± 0.513

4.908 ± 1.002

3.286 ± 0.66

7.554 ± 0.627

1.921 ± 0.443

4.524 ± 0.343

4.311 ± 0.746

9.134 ± 0.7

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.988 ± 0.445

2.774 ± 0.39

4.097 ± 0.87

4.055 ± 0.438

7.341 ± 1.196

6.146 ± 1.04

5.548 ± 0.938

7.384 ± 0.526

2.177 ± 0.325

2.774 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski