Wheat leaf yellowing-associated virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus; unclassified Polerovirus

Average proteome isoelectric point is 7.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A220T7N1|A0A220T7N1_9LUTE Readthrough protein OS=Wheat leaf yellowing-associated virus OX=2019445 GN=WLYaV_gp5 PE=3 SV=1
MM1 pKa = 7.48LNAEE5 pKa = 3.9ITAEE9 pKa = 4.1GFVFTGPATSAPATHH24 pKa = 6.94EE25 pKa = 4.15DD26 pKa = 3.75VEE28 pKa = 4.78HH29 pKa = 6.66FLVTYY34 pKa = 10.23RR35 pKa = 11.84RR36 pKa = 11.84SLFLWIHH43 pKa = 5.6EE44 pKa = 4.46CSPFDD49 pKa = 4.08TIRR52 pKa = 11.84VIHH55 pKa = 6.73KK56 pKa = 9.85YY57 pKa = 10.99GSFHH61 pKa = 6.41KK62 pKa = 10.13WGLAVRR68 pKa = 11.84AYY70 pKa = 10.55LCAFIPIILLPGTTINAEE88 pKa = 4.41LISADD93 pKa = 3.72GIFRR97 pKa = 11.84KK98 pKa = 9.95SIYY101 pKa = 9.87VWLARR106 pKa = 11.84FKK108 pKa = 9.66QHH110 pKa = 5.69YY111 pKa = 9.24RR112 pKa = 11.84IASRR116 pKa = 11.84RR117 pKa = 11.84GRR119 pKa = 11.84CLIYY123 pKa = 10.27HH124 pKa = 6.44KK125 pKa = 10.84QPDD128 pKa = 3.73GSFEE132 pKa = 4.83SNLWRR137 pKa = 11.84AVWAPEE143 pKa = 3.89LQSVEE148 pKa = 3.76RR149 pKa = 11.84FKK151 pKa = 11.11RR152 pKa = 11.84ITEE155 pKa = 4.08RR156 pKa = 11.84GLEE159 pKa = 4.12SFKK162 pKa = 10.68TALNLDD168 pKa = 3.77ILPLEE173 pKa = 4.31RR174 pKa = 11.84VHH176 pKa = 7.99KK177 pKa = 9.93IICGKK182 pKa = 9.32PALDD186 pKa = 4.67DD187 pKa = 4.26DD188 pKa = 4.37QVISILSWYY197 pKa = 10.26DD198 pKa = 4.06DD199 pKa = 3.56ILDD202 pKa = 4.88GYY204 pKa = 10.76DD205 pKa = 3.63SEE207 pKa = 5.32DD208 pKa = 3.62NPVEE212 pKa = 4.04TRR214 pKa = 11.84AGMRR218 pKa = 11.84LFGCGFYY225 pKa = 11.01NDD227 pKa = 4.61LLDD230 pKa = 3.87VEE232 pKa = 4.95KK233 pKa = 11.04DD234 pKa = 3.64LLIHH238 pKa = 6.36SFMVGWSSTHH248 pKa = 6.28SPFYY252 pKa = 11.21DD253 pKa = 3.25NTLVGQTADD262 pKa = 3.56QIRR265 pKa = 11.84IWGEE269 pKa = 3.29QWRR272 pKa = 4.06

Molecular weight: 31.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A220T7N5|A0A220T7N5_9LUTE Movement protein OS=Wheat leaf yellowing-associated virus OX=2019445 GN=WLYaV_gp7 PE=3 SV=1
MM1 pKa = 6.87NTGGNRR7 pKa = 11.84TRR9 pKa = 11.84RR10 pKa = 11.84NARR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ANRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84STRR23 pKa = 11.84PVVVVRR29 pKa = 11.84PTPKK33 pKa = 8.88PRR35 pKa = 11.84RR36 pKa = 11.84VRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84ASAGGGAVRR50 pKa = 11.84GPGGRR55 pKa = 11.84SNRR58 pKa = 11.84EE59 pKa = 3.59VFTFTVDD66 pKa = 3.41DD67 pKa = 4.61LKK69 pKa = 11.6ANSSGILKK77 pKa = 9.99FGPNLSQYY85 pKa = 11.26AAFNNGILKK94 pKa = 10.24AYY96 pKa = 9.77HH97 pKa = 6.5EE98 pKa = 4.42YY99 pKa = 10.71KK100 pKa = 9.38ITSLTIQYY108 pKa = 9.32NSCSSSTTSGAIALEE123 pKa = 4.21VDD125 pKa = 4.38TSCSQTTTGSKK136 pKa = 9.73IVSFPVKK143 pKa = 10.49SNTRR147 pKa = 11.84KK148 pKa = 7.85TFPTSFIRR156 pKa = 11.84GKK158 pKa = 10.76DD159 pKa = 3.74FVTTTADD166 pKa = 4.14QFWLLYY172 pKa = 9.95KK173 pKa = 11.05GNGDD177 pKa = 3.49SSLAGQFVCRR187 pKa = 11.84FEE189 pKa = 5.38CQFQNPKK196 pKa = 10.4

Molecular weight: 21.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3004

44

1045

429.1

47.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.691 ± 0.275

1.731 ± 0.234

4.128 ± 0.403

6.092 ± 0.65

3.395 ± 0.441

7.057 ± 0.489

2.031 ± 0.402

4.727 ± 0.589

6.059 ± 0.591

8.722 ± 0.902

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.447

4.294 ± 0.591

5.726 ± 0.472

3.862 ± 0.568

5.659 ± 1.081

9.92 ± 0.709

6.192 ± 0.636

5.659 ± 0.226

2.031 ± 0.376

3.795 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski