Rubripirellula tenax
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6268 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C6EIR8|A0A5C6EIR8_9BACT Outer membrane biogenesis protein BamB OS=Rubripirellula tenax OX=2528015 GN=Poly51_45890 PE=4 SV=1
MM1 pKa = 7.65 SDD3 pKa = 2.9 VTVYY7 pKa = 11.11 DD8 pKa = 3.28 KK9 pKa = 11.55 AKK11 pKa = 8.14 WHH13 pKa = 6.64 FGGDD17 pKa = 3.55 YY18 pKa = 10.61 PADD21 pKa = 3.93 LDD23 pKa = 3.73 ADD25 pKa = 3.9 YY26 pKa = 10.76 AYY28 pKa = 9.25 THH30 pKa = 7.03 AGYY33 pKa = 10.39 FFGWIVDD40 pKa = 3.68 NDD42 pKa = 4.02 LVDD45 pKa = 5.05 SDD47 pKa = 4.55 FLDD50 pKa = 5.46 DD51 pKa = 5.08 IGDD54 pKa = 4.16 EE55 pKa = 4.04 IARR58 pKa = 11.84 FRR60 pKa = 11.84 ARR62 pKa = 11.84 EE63 pKa = 3.49 ISARR67 pKa = 11.84 EE68 pKa = 3.75 LFIITDD74 pKa = 3.92 GALVDD79 pKa = 5.94 DD80 pKa = 4.71 MMNDD84 pKa = 3.21 TGNAFAAHH92 pKa = 6.33 YY93 pKa = 10.57 FDD95 pKa = 4.42 FTNGQYY101 pKa = 10.93 LADD104 pKa = 4.31 YY105 pKa = 10.16 EE106 pKa = 5.0 SLLCDD111 pKa = 4.36 GLPTMYY117 pKa = 10.16 HH118 pKa = 6.99 AEE120 pKa = 4.19 DD121 pKa = 3.66 TPEE124 pKa = 3.98 NYY126 pKa = 7.74 EE127 pKa = 4.27 TICAKK132 pKa = 8.71 ITEE135 pKa = 5.13 RR136 pKa = 11.84 FDD138 pKa = 2.85 EE139 pKa = 4.59 WNASRR144 pKa = 5.08
Molecular weight: 16.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.859
IPC_protein 3.872
Toseland 3.643
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.834
Rodwell 3.694
Grimsley 3.554
Solomon 3.872
Lehninger 3.821
Nozaki 3.986
DTASelect 4.266
Thurlkill 3.706
EMBOSS 3.834
Sillero 3.999
Patrickios 0.846
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.873
Protein with the highest isoelectric point:
>tr|A0A5C6EM21|A0A5C6EM21_9BACT Uncharacterized protein OS=Rubripirellula tenax OX=2528015 GN=Poly51_45620 PE=4 SV=1
MM1 pKa = 7.23 FSKK4 pKa = 10.47 RR5 pKa = 11.84 KK6 pKa = 9.42 HH7 pKa = 5.98 LPTVALVGLPLVGTCVGVALGAIYY31 pKa = 9.59 PPRR34 pKa = 11.84 NPIDD38 pKa = 3.43 YY39 pKa = 8.78 WPTIGFLGAGFAVVGLLFGVLTWLWMRR66 pKa = 11.84 GRR68 pKa = 11.84 VSAVVALSAIAIPACTFGWFINGKK92 pKa = 9.03 GGRR95 pKa = 11.84 WDD97 pKa = 3.61 NAWWFGLVGFIACVLFFVRR116 pKa = 11.84 SANLIANRR124 pKa = 11.84 DD125 pKa = 3.09 RR126 pKa = 11.84 DD127 pKa = 3.72 ARR129 pKa = 11.84 GGG131 pKa = 3.52
Molecular weight: 14.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.648
IPC_protein 10.555
Toseland 10.628
ProMoST 10.394
Dawson 10.745
Bjellqvist 10.511
Wikipedia 10.979
Rodwell 10.877
Grimsley 10.804
Solomon 10.891
Lehninger 10.847
Nozaki 10.672
DTASelect 10.482
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.687
Patrickios 10.672
IPC_peptide 10.891
IPC2_peptide 9.882
IPC2.peptide.svr19 8.445
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6268
0
6268
2351484
29
12012
375.2
41.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.769 ± 0.031
1.073 ± 0.014
6.702 ± 0.036
5.616 ± 0.033
3.785 ± 0.018
7.828 ± 0.047
2.134 ± 0.02
5.325 ± 0.022
3.523 ± 0.036
9.07 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.409 ± 0.022
3.35 ± 0.033
5.039 ± 0.028
3.65 ± 0.019
6.579 ± 0.038
6.985 ± 0.029
5.963 ± 0.052
7.414 ± 0.026
1.466 ± 0.015
2.318 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here