Amino acid dipepetide frequency for Bacillus virus Andromeda

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.305AlaAla: 2.305 ± 0.797
0.61AlaCys: 0.61 ± 0.219
3.39AlaAsp: 3.39 ± 0.534
3.662AlaGlu: 3.662 ± 0.408
3.662AlaPhe: 3.662 ± 0.38
4.611AlaGly: 4.611 ± 0.718
0.881AlaHis: 0.881 ± 0.253
4.746AlaIle: 4.746 ± 0.821
4.95AlaLys: 4.95 ± 0.588
5.085AlaLeu: 5.085 ± 0.731
2.238AlaMet: 2.238 ± 0.46
3.051AlaAsn: 3.051 ± 0.449
2.509AlaPro: 2.509 ± 0.461
2.305AlaGln: 2.305 ± 0.379
2.916AlaArg: 2.916 ± 0.556
3.729AlaSer: 3.729 ± 0.503
3.255AlaThr: 3.255 ± 0.558
4.136AlaVal: 4.136 ± 0.499
1.017AlaTrp: 1.017 ± 0.309
3.051AlaTyr: 3.051 ± 0.353
0.0AlaXaa: 0.0 ± 0.0
Cys
0.407CysAla: 0.407 ± 0.172
0.203CysCys: 0.203 ± 0.094
0.61CysAsp: 0.61 ± 0.171
0.475CysGlu: 0.475 ± 0.188
0.61CysPhe: 0.61 ± 0.193
0.678CysGly: 0.678 ± 0.208
0.407CysHis: 0.407 ± 0.197
0.407CysIle: 0.407 ± 0.158
1.085CysLys: 1.085 ± 0.316
0.746CysLeu: 0.746 ± 0.212
0.136CysMet: 0.136 ± 0.1
0.271CysAsn: 0.271 ± 0.13
0.339CysPro: 0.339 ± 0.155
0.339CysGln: 0.339 ± 0.122
0.271CysArg: 0.271 ± 0.118
0.61CysSer: 0.61 ± 0.191
0.881CysThr: 0.881 ± 0.22
0.136CysVal: 0.136 ± 0.101
0.068CysTrp: 0.068 ± 0.077
0.475CysTyr: 0.475 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
3.865AspAla: 3.865 ± 0.589
0.746AspCys: 0.746 ± 0.289
3.39AspAsp: 3.39 ± 0.476
4.136AspGlu: 4.136 ± 0.561
3.526AspPhe: 3.526 ± 0.551
4.068AspGly: 4.068 ± 0.594
1.56AspHis: 1.56 ± 0.323
4.95AspIle: 4.95 ± 0.635
5.424AspLys: 5.424 ± 0.601
5.085AspLeu: 5.085 ± 0.447
1.424AspMet: 1.424 ± 0.31
2.373AspAsn: 2.373 ± 0.376
3.051AspPro: 3.051 ± 0.394
2.102AspGln: 2.102 ± 0.326
3.594AspArg: 3.594 ± 0.47
3.051AspSer: 3.051 ± 0.456
2.848AspThr: 2.848 ± 0.554
4.204AspVal: 4.204 ± 0.473
0.949AspTrp: 0.949 ± 0.261
2.916AspTyr: 2.916 ± 0.387
0.0AspXaa: 0.0 ± 0.0
Glu
5.357GluAla: 5.357 ± 0.738
0.339GluCys: 0.339 ± 0.153
4.204GluAsp: 4.204 ± 0.607
8.34GluGlu: 8.34 ± 1.18
3.933GluPhe: 3.933 ± 0.529
4.611GluGly: 4.611 ± 0.498
1.627GluHis: 1.627 ± 0.341
4.068GluIle: 4.068 ± 0.542
5.221GluLys: 5.221 ± 0.74
8.747GluLeu: 8.747 ± 1.052
2.373GluMet: 2.373 ± 0.442
3.255GluAsn: 3.255 ± 0.574
1.695GluPro: 1.695 ± 0.378
3.865GluGln: 3.865 ± 0.531
3.458GluArg: 3.458 ± 0.463
4.136GluSer: 4.136 ± 0.495
3.933GluThr: 3.933 ± 0.502
4.95GluVal: 4.95 ± 0.72
0.814GluTrp: 0.814 ± 0.202
3.458GluTyr: 3.458 ± 0.495
0.0GluXaa: 0.0 ± 0.0
Phe
2.983PheAla: 2.983 ± 0.547
0.339PheCys: 0.339 ± 0.158
3.662PheAsp: 3.662 ± 0.411
3.526PheGlu: 3.526 ± 0.541
1.424PhePhe: 1.424 ± 0.376
2.848PheGly: 2.848 ± 0.392
1.017PheHis: 1.017 ± 0.266
3.255PheIle: 3.255 ± 0.419
3.119PheLys: 3.119 ± 0.456
3.797PheLeu: 3.797 ± 0.489
1.627PheMet: 1.627 ± 0.351
1.899PheAsn: 1.899 ± 0.33
1.492PhePro: 1.492 ± 0.333
1.424PheGln: 1.424 ± 0.289
1.695PheArg: 1.695 ± 0.331
2.102PheSer: 2.102 ± 0.39
3.933PheThr: 3.933 ± 0.57
2.644PheVal: 2.644 ± 0.33
0.271PheTrp: 0.271 ± 0.147
2.034PheTyr: 2.034 ± 0.342
0.0PheXaa: 0.0 ± 0.0
Gly
5.018GlyAla: 5.018 ± 0.697
0.678GlyCys: 0.678 ± 0.213
5.153GlyAsp: 5.153 ± 0.562
5.018GlyGlu: 5.018 ± 0.489
3.187GlyPhe: 3.187 ± 0.397
4.679GlyGly: 4.679 ± 0.631
0.881GlyHis: 0.881 ± 0.27
4.543GlyIle: 4.543 ± 0.522
5.492GlyLys: 5.492 ± 0.676
5.221GlyLeu: 5.221 ± 0.67
1.831GlyMet: 1.831 ± 0.369
3.729GlyAsn: 3.729 ± 0.852
0.136GlyPro: 0.136 ± 0.124
2.17GlyGln: 2.17 ± 0.511
3.322GlyArg: 3.322 ± 0.447
4.611GlySer: 4.611 ± 0.66
3.729GlyThr: 3.729 ± 0.466
4.475GlyVal: 4.475 ± 0.707
0.949GlyTrp: 0.949 ± 0.227
2.441GlyTyr: 2.441 ± 0.347
0.0GlyXaa: 0.0 ± 0.0
His
0.949HisAla: 0.949 ± 0.251
0.203HisCys: 0.203 ± 0.152
0.881HisAsp: 0.881 ± 0.246
1.356HisGlu: 1.356 ± 0.302
1.356HisPhe: 1.356 ± 0.295
1.831HisGly: 1.831 ± 0.419
0.407HisHis: 0.407 ± 0.139
1.695HisIle: 1.695 ± 0.353
1.424HisLys: 1.424 ± 0.296
1.763HisLeu: 1.763 ± 0.442
0.475HisMet: 0.475 ± 0.166
0.881HisAsn: 0.881 ± 0.205
0.746HisPro: 0.746 ± 0.175
0.881HisGln: 0.881 ± 0.232
0.814HisArg: 0.814 ± 0.235
1.627HisSer: 1.627 ± 0.362
0.949HisThr: 0.949 ± 0.268
0.881HisVal: 0.881 ± 0.288
0.339HisTrp: 0.339 ± 0.143
1.017HisTyr: 1.017 ± 0.307
0.0HisXaa: 0.0 ± 0.0
Ile
4.882IleAla: 4.882 ± 0.532
0.678IleCys: 0.678 ± 0.236
4.95IleAsp: 4.95 ± 0.619
5.221IleGlu: 5.221 ± 0.529
2.238IlePhe: 2.238 ± 0.411
4.272IleGly: 4.272 ± 0.722
1.695IleHis: 1.695 ± 0.313
4.272IleIle: 4.272 ± 0.625
5.967IleLys: 5.967 ± 0.727
4.611IleLeu: 4.611 ± 0.567
2.034IleMet: 2.034 ± 0.32
3.458IleAsn: 3.458 ± 0.454
2.509IlePro: 2.509 ± 0.357
2.509IleGln: 2.509 ± 0.381
3.255IleArg: 3.255 ± 0.431
3.933IleSer: 3.933 ± 0.506
4.34IleThr: 4.34 ± 0.53
4.068IleVal: 4.068 ± 0.574
0.407IleTrp: 0.407 ± 0.127
2.034IleTyr: 2.034 ± 0.407
0.0IleXaa: 0.0 ± 0.0
Lys
4.746LysAla: 4.746 ± 0.689
0.542LysCys: 0.542 ± 0.227
5.357LysAsp: 5.357 ± 0.784
7.323LysGlu: 7.323 ± 0.936
3.458LysPhe: 3.458 ± 0.598
4.679LysGly: 4.679 ± 0.771
2.577LysHis: 2.577 ± 0.392
3.594LysIle: 3.594 ± 0.512
7.662LysLys: 7.662 ± 1.098
5.763LysLeu: 5.763 ± 0.586
2.373LysMet: 2.373 ± 0.379
3.797LysAsn: 3.797 ± 0.495
3.051LysPro: 3.051 ± 0.421
4.136LysGln: 4.136 ± 0.533
4.407LysArg: 4.407 ± 0.703
4.272LysSer: 4.272 ± 0.607
4.136LysThr: 4.136 ± 0.509
5.424LysVal: 5.424 ± 0.628
0.542LysTrp: 0.542 ± 0.179
3.119LysTyr: 3.119 ± 0.463
0.0LysXaa: 0.0 ± 0.0
Leu
4.34LeuAla: 4.34 ± 0.656
0.881LeuCys: 0.881 ± 0.265
5.018LeuAsp: 5.018 ± 0.602
6.509LeuGlu: 6.509 ± 0.77
2.373LeuPhe: 2.373 ± 0.365
5.831LeuGly: 5.831 ± 0.701
1.492LeuHis: 1.492 ± 0.294
5.153LeuIle: 5.153 ± 0.584
7.187LeuLys: 7.187 ± 0.815
5.831LeuLeu: 5.831 ± 0.614
1.831LeuMet: 1.831 ± 0.343
4.204LeuAsn: 4.204 ± 0.604
2.848LeuPro: 2.848 ± 0.465
2.305LeuGln: 2.305 ± 0.427
3.729LeuArg: 3.729 ± 0.419
5.763LeuSer: 5.763 ± 0.667
5.492LeuThr: 5.492 ± 0.611
4.882LeuVal: 4.882 ± 0.69
0.61LeuTrp: 0.61 ± 0.19
3.051LeuTyr: 3.051 ± 0.58
0.0LeuXaa: 0.0 ± 0.0
Met
2.238MetAla: 2.238 ± 0.412
0.271MetCys: 0.271 ± 0.109
1.627MetAsp: 1.627 ± 0.352
1.492MetGlu: 1.492 ± 0.273
1.356MetPhe: 1.356 ± 0.294
1.695MetGly: 1.695 ± 0.342
0.475MetHis: 0.475 ± 0.196
1.763MetIle: 1.763 ± 0.335
2.712MetLys: 2.712 ± 0.469
1.966MetLeu: 1.966 ± 0.392
0.814MetMet: 0.814 ± 0.281
1.085MetAsn: 1.085 ± 0.278
0.881MetPro: 0.881 ± 0.291
0.814MetGln: 0.814 ± 0.24
1.017MetArg: 1.017 ± 0.261
3.119MetSer: 3.119 ± 0.462
1.899MetThr: 1.899 ± 0.328
1.221MetVal: 1.221 ± 0.282
0.203MetTrp: 0.203 ± 0.142
0.949MetTyr: 0.949 ± 0.263
0.0MetXaa: 0.0 ± 0.0
Asn
2.712AsnAla: 2.712 ± 0.484
0.61AsnCys: 0.61 ± 0.227
2.577AsnAsp: 2.577 ± 0.425
3.797AsnGlu: 3.797 ± 0.524
2.17AsnPhe: 2.17 ± 0.306
4.136AsnGly: 4.136 ± 0.626
0.949AsnHis: 0.949 ± 0.226
3.39AsnIle: 3.39 ± 0.519
3.865AsnLys: 3.865 ± 0.41
3.594AsnLeu: 3.594 ± 0.441
1.695AsnMet: 1.695 ± 0.373
2.983AsnAsn: 2.983 ± 0.484
1.831AsnPro: 1.831 ± 0.319
1.492AsnGln: 1.492 ± 0.319
1.831AsnArg: 1.831 ± 0.361
2.712AsnSer: 2.712 ± 0.43
3.39AsnThr: 3.39 ± 0.615
3.526AsnVal: 3.526 ± 0.589
0.678AsnTrp: 0.678 ± 0.193
1.627AsnTyr: 1.627 ± 0.285
0.0AsnXaa: 0.0 ± 0.0
Pro
2.644ProAla: 2.644 ± 0.421
0.271ProCys: 0.271 ± 0.148
2.644ProAsp: 2.644 ± 0.443
3.187ProGlu: 3.187 ± 0.519
1.831ProPhe: 1.831 ± 0.337
0.0ProGly: 0.0 ± 0.0
0.814ProHis: 0.814 ± 0.247
2.238ProIle: 2.238 ± 0.411
2.238ProLys: 2.238 ± 0.352
3.39ProLeu: 3.39 ± 0.512
1.085ProMet: 1.085 ± 0.26
1.356ProAsn: 1.356 ± 0.328
0.949ProPro: 0.949 ± 0.307
0.814ProGln: 0.814 ± 0.231
1.424ProArg: 1.424 ± 0.3
2.238ProSer: 2.238 ± 0.461
1.695ProThr: 1.695 ± 0.367
1.763ProVal: 1.763 ± 0.328
0.339ProTrp: 0.339 ± 0.164
0.61ProTyr: 0.61 ± 0.192
0.0ProXaa: 0.0 ± 0.0
Gln
2.17GlnAla: 2.17 ± 0.324
0.271GlnCys: 0.271 ± 0.13
2.17GlnAsp: 2.17 ± 0.456
3.933GlnGlu: 3.933 ± 0.627
1.627GlnPhe: 1.627 ± 0.3
2.238GlnGly: 2.238 ± 0.374
0.475GlnHis: 0.475 ± 0.175
2.305GlnIle: 2.305 ± 0.353
2.916GlnLys: 2.916 ± 0.484
3.458GlnLeu: 3.458 ± 0.438
1.153GlnMet: 1.153 ± 0.261
1.288GlnAsn: 1.288 ± 0.326
1.288GlnPro: 1.288 ± 0.281
0.949GlnGln: 0.949 ± 0.378
1.763GlnArg: 1.763 ± 0.376
2.441GlnSer: 2.441 ± 0.418
1.627GlnThr: 1.627 ± 0.381
2.78GlnVal: 2.78 ± 0.482
0.542GlnTrp: 0.542 ± 0.203
1.356GlnTyr: 1.356 ± 0.35
0.0GlnXaa: 0.0 ± 0.0
Arg
3.187ArgAla: 3.187 ± 0.465
0.271ArgCys: 0.271 ± 0.129
3.119ArgAsp: 3.119 ± 0.383
3.526ArgGlu: 3.526 ± 0.53
1.966ArgPhe: 1.966 ± 0.369
2.712ArgGly: 2.712 ± 0.379
0.746ArgHis: 0.746 ± 0.202
3.458ArgIle: 3.458 ± 0.461
3.119ArgLys: 3.119 ± 0.439
3.458ArgLeu: 3.458 ± 0.665
0.881ArgMet: 0.881 ± 0.234
2.034ArgAsn: 2.034 ± 0.353
1.221ArgPro: 1.221 ± 0.328
1.966ArgGln: 1.966 ± 0.363
3.051ArgArg: 3.051 ± 0.427
2.441ArgSer: 2.441 ± 0.385
2.373ArgThr: 2.373 ± 0.491
3.187ArgVal: 3.187 ± 0.517
0.542ArgTrp: 0.542 ± 0.178
1.763ArgTyr: 1.763 ± 0.41
0.0ArgXaa: 0.0 ± 0.0
Ser
3.255SerAla: 3.255 ± 0.629
0.61SerCys: 0.61 ± 0.256
3.39SerAsp: 3.39 ± 0.589
4.068SerGlu: 4.068 ± 0.405
2.983SerPhe: 2.983 ± 0.471
5.289SerGly: 5.289 ± 0.706
1.627SerHis: 1.627 ± 0.342
5.018SerIle: 5.018 ± 0.601
5.56SerLys: 5.56 ± 0.617
4.679SerLeu: 4.679 ± 0.652
1.288SerMet: 1.288 ± 0.263
4.204SerAsn: 4.204 ± 0.687
1.627SerPro: 1.627 ± 0.365
2.916SerGln: 2.916 ± 0.421
2.102SerArg: 2.102 ± 0.319
4.543SerSer: 4.543 ± 0.781
2.916SerThr: 2.916 ± 0.584
4.001SerVal: 4.001 ± 0.549
0.881SerTrp: 0.881 ± 0.304
1.56SerTyr: 1.56 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
4.204ThrAla: 4.204 ± 0.62
0.339ThrCys: 0.339 ± 0.138
3.119ThrAsp: 3.119 ± 0.544
5.018ThrGlu: 5.018 ± 0.484
2.916ThrPhe: 2.916 ± 0.408
4.95ThrGly: 4.95 ± 0.655
0.746ThrHis: 0.746 ± 0.256
4.001ThrIle: 4.001 ± 0.588
3.797ThrLys: 3.797 ± 0.514
3.933ThrLeu: 3.933 ± 0.635
1.153ThrMet: 1.153 ± 0.261
3.255ThrAsn: 3.255 ± 0.608
2.78ThrPro: 2.78 ± 0.511
1.627ThrGln: 1.627 ± 0.302
2.238ThrArg: 2.238 ± 0.418
3.662ThrSer: 3.662 ± 1.039
3.051ThrThr: 3.051 ± 0.552
4.068ThrVal: 4.068 ± 0.475
0.949ThrTrp: 0.949 ± 0.339
2.848ThrTyr: 2.848 ± 0.463
0.0ThrXaa: 0.0 ± 0.0
Val
4.475ValAla: 4.475 ± 0.563
0.881ValCys: 0.881 ± 0.243
4.068ValAsp: 4.068 ± 0.536
4.136ValGlu: 4.136 ± 0.557
2.577ValPhe: 2.577 ± 0.421
3.797ValGly: 3.797 ± 0.612
1.085ValHis: 1.085 ± 0.239
5.221ValIle: 5.221 ± 0.716
5.357ValLys: 5.357 ± 0.655
4.407ValLeu: 4.407 ± 0.688
1.831ValMet: 1.831 ± 0.318
3.187ValAsn: 3.187 ± 0.418
2.034ValPro: 2.034 ± 0.287
2.712ValGln: 2.712 ± 0.401
2.17ValArg: 2.17 ± 0.37
3.797ValSer: 3.797 ± 0.591
4.475ValThr: 4.475 ± 0.621
4.407ValVal: 4.407 ± 0.569
0.475ValTrp: 0.475 ± 0.172
3.187ValTyr: 3.187 ± 0.406
0.0ValXaa: 0.0 ± 0.0
Trp
0.339TrpAla: 0.339 ± 0.129
0.203TrpCys: 0.203 ± 0.09
0.678TrpAsp: 0.678 ± 0.195
0.678TrpGlu: 0.678 ± 0.246
0.407TrpPhe: 0.407 ± 0.182
0.678TrpGly: 0.678 ± 0.185
0.271TrpHis: 0.271 ± 0.117
0.61TrpIle: 0.61 ± 0.156
0.881TrpLys: 0.881 ± 0.237
0.949TrpLeu: 0.949 ± 0.237
0.407TrpMet: 0.407 ± 0.151
0.881TrpAsn: 0.881 ± 0.32
0.0TrpPro: 0.0 ± 0.0
0.203TrpGln: 0.203 ± 0.115
0.475TrpArg: 0.475 ± 0.221
1.085TrpSer: 1.085 ± 0.327
0.746TrpThr: 0.746 ± 0.235
0.814TrpVal: 0.814 ± 0.259
0.271TrpTrp: 0.271 ± 0.149
0.61TrpTyr: 0.61 ± 0.158
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.034TyrAla: 2.034 ± 0.4
0.203TyrCys: 0.203 ± 0.115
3.255TyrAsp: 3.255 ± 0.488
2.983TyrGlu: 2.983 ± 0.342
1.492TyrPhe: 1.492 ± 0.304
3.729TyrGly: 3.729 ± 0.472
0.746TyrHis: 0.746 ± 0.222
2.644TyrIle: 2.644 ± 0.414
2.983TyrLys: 2.983 ± 0.474
2.848TyrLeu: 2.848 ± 0.41
0.881TyrMet: 0.881 ± 0.243
2.373TyrAsn: 2.373 ± 0.486
0.542TyrPro: 0.542 ± 0.261
1.221TyrGln: 1.221 ± 0.238
1.492TyrArg: 1.492 ± 0.496
2.644TyrSer: 2.644 ± 0.378
2.983TyrThr: 2.983 ± 0.497
2.712TyrVal: 2.712 ± 0.423
0.339TyrTrp: 0.339 ± 0.143
1.424TyrTyr: 1.424 ± 0.328
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (14749 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski