Infectious laryngotracheitis virus (ILTV) (Gallid herpesvirus 1)
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q67644|Q67644_ILTV Envelope glycoprotein D OS=Infectious laryngotracheitis virus OX=10386 GN=US6 PE=4 SV=1
MM1 pKa = 7.57 SGFSNIGSIATVSLVCSLLCASVLGAPVLDD31 pKa = 4.33 GLEE34 pKa = 4.13 SSPFPFGGKK43 pKa = 9.7 IIAQACNRR51 pKa = 11.84 TTIEE55 pKa = 4.04 VTVPWSDD62 pKa = 3.39 YY63 pKa = 10.44 SGRR66 pKa = 11.84 TEE68 pKa = 4.1 GVSVEE73 pKa = 4.37 VKK75 pKa = 9.29 WFYY78 pKa = 11.61 GNSNPEE84 pKa = 3.97 SFVFGVDD91 pKa = 3.2 SEE93 pKa = 4.72 TGSGHH98 pKa = 6.8 EE99 pKa = 4.98 DD100 pKa = 4.98 LSTCWALIHH109 pKa = 6.21 NLNASVCRR117 pKa = 11.84 ASDD120 pKa = 3.18 AGIPDD125 pKa = 4.04 FDD127 pKa = 4.05 KK128 pKa = 11.16 QCEE131 pKa = 4.12 KK132 pKa = 10.0 VQRR135 pKa = 11.84 RR136 pKa = 11.84 LRR138 pKa = 11.84 SGVEE142 pKa = 3.48 LGSYY146 pKa = 10.34 VSGNGSLVLYY156 pKa = 9.96 PGMYY160 pKa = 10.18 DD161 pKa = 3.14 AGIYY165 pKa = 9.99 AYY167 pKa = 10.0 QLSVGGKK174 pKa = 8.44 GYY176 pKa = 9.07 TGSVYY181 pKa = 10.77 LDD183 pKa = 3.26 VGPNPGCHH191 pKa = 6.13 DD192 pKa = 3.6 QYY194 pKa = 11.66 GYY196 pKa = 9.51 TYY198 pKa = 11.07 YY199 pKa = 11.28 SLADD203 pKa = 3.66 EE204 pKa = 5.21 ASDD207 pKa = 3.74 LSSYY211 pKa = 11.19 DD212 pKa = 3.37 VASPEE217 pKa = 3.9 LDD219 pKa = 3.2 GPMEE223 pKa = 4.39 EE224 pKa = 5.73 DD225 pKa = 3.7 YY226 pKa = 11.61 SNCLDD231 pKa = 4.08 MPPLRR236 pKa = 11.84 PWTTVCSHH244 pKa = 6.89 DD245 pKa = 4.71 VEE247 pKa = 4.43 EE248 pKa = 4.8 QEE250 pKa = 4.34 NATDD254 pKa = 5.25 EE255 pKa = 4.67 LYY257 pKa = 10.97 LWDD260 pKa = 4.54 EE261 pKa = 4.48 EE262 pKa = 4.81 CAGPLDD268 pKa = 4.09 EE269 pKa = 5.5 YY270 pKa = 11.28 VDD272 pKa = 3.96 EE273 pKa = 5.45 RR274 pKa = 11.84 SEE276 pKa = 4.07 TMPRR280 pKa = 11.84 MVVFSPPSTLQQQ292 pKa = 3.16
Molecular weight: 31.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.951
IPC2_protein 4.012
IPC_protein 3.986
Toseland 3.795
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.859
Rodwell 3.821
Grimsley 3.706
Solomon 3.948
Lehninger 3.91
Nozaki 4.062
DTASelect 4.253
Thurlkill 3.821
EMBOSS 3.872
Sillero 4.101
Patrickios 0.197
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 4.011
Protein with the highest isoelectric point:
>tr|Q98111|Q98111_ILTV Isoform of F5B4W6 Protein LORF2 OS=Infectious laryngotracheitis virus OX=10386 GN=UL[-1] PE=4 SV=2
MM1 pKa = 7.99 KK2 pKa = 10.27 YY3 pKa = 10.02 GQAAIATDD11 pKa = 3.44 MDD13 pKa = 4.61 FARR16 pKa = 11.84 MSRR19 pKa = 11.84 QPPRR23 pKa = 11.84 KK24 pKa = 9.47 NSRR27 pKa = 11.84 CSSARR32 pKa = 11.84 TAALQGNGYY41 pKa = 9.91 CFLPKK46 pKa = 9.78 SEE48 pKa = 5.24 KK49 pKa = 10.71 LPEE52 pKa = 4.22 LPSRR56 pKa = 11.84 HH57 pKa = 5.81 FEE59 pKa = 4.11 TRR61 pKa = 11.84 FSSLLPPAAAKK72 pKa = 10.22
Molecular weight: 7.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.275
IPC2_protein 9.443
IPC_protein 9.677
Toseland 10.335
ProMoST 10.058
Dawson 10.467
Bjellqvist 10.131
Wikipedia 10.628
Rodwell 10.877
Grimsley 10.511
Solomon 10.526
Lehninger 10.511
Nozaki 10.365
DTASelect 10.116
Thurlkill 10.35
EMBOSS 10.73
Sillero 10.394
Patrickios 10.672
IPC_peptide 10.54
IPC2_peptide 9.107
IPC2.peptide.svr19 8.435
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
68
7
75
39631
72
2784
528.4
59.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.979 ± 0.203
2.162 ± 0.121
5.19 ± 0.162
6.399 ± 0.212
4.401 ± 0.172
5.617 ± 0.228
2.233 ± 0.078
5.526 ± 0.205
4.464 ± 0.236
9.702 ± 0.203
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.162 ± 0.086
3.739 ± 0.112
5.708 ± 0.286
3.101 ± 0.119
6.543 ± 0.259
8.567 ± 0.192
6.192 ± 0.256
5.955 ± 0.149
1.153 ± 0.066
3.207 ± 0.107
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here