Halopelagius longus
Average proteome isoelectric point is 4.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3802 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H1BB56|A0A1H1BB56_9EURY Sugar or nucleoside kinase ribokinase family OS=Halopelagius longus OX=1236180 GN=DWB78_02675 PE=4 SV=1
MM1 pKa = 7.35 SIVVGGAAFGIFYY14 pKa = 8.23 GTVVSSSRR22 pKa = 11.84 RR23 pKa = 11.84 LMTMEE28 pKa = 4.33 LTWHH32 pKa = 6.17 GHH34 pKa = 4.56 STWHH38 pKa = 6.21 VDD40 pKa = 3.02 VDD42 pKa = 3.79 GTTFLIDD49 pKa = 3.54 PFFDD53 pKa = 3.79 NPHH56 pKa = 6.37 TDD58 pKa = 4.01 LEE60 pKa = 4.64 PEE62 pKa = 4.13 DD63 pKa = 5.25 VEE65 pKa = 4.58 TPDD68 pKa = 4.52 YY69 pKa = 11.42 LLLTHH74 pKa = 6.38 GHH76 pKa = 7.17 ADD78 pKa = 3.89 HH79 pKa = 6.89 IADD82 pKa = 3.26 VDD84 pKa = 3.9 AFTDD88 pKa = 3.51 ATLVAVPEE96 pKa = 4.35 MTAFMQDD103 pKa = 2.64 EE104 pKa = 4.82 HH105 pKa = 8.35 GFEE108 pKa = 4.69 DD109 pKa = 5.39 AIGMNLGGTTEE120 pKa = 4.48 CGDD123 pKa = 3.89 AFVTMHH129 pKa = 7.22 RR130 pKa = 11.84 ADD132 pKa = 3.55 HH133 pKa = 6.09 TNGLNTSYY141 pKa = 10.48 EE142 pKa = 4.05 NEE144 pKa = 3.99 IGVPAGFVISDD155 pKa = 3.54 EE156 pKa = 4.62 RR157 pKa = 11.84 PSQDD161 pKa = 3.39 EE162 pKa = 4.38 DD163 pKa = 3.89 DD164 pKa = 4.48 GATAFYY170 pKa = 10.37 HH171 pKa = 6.94 AGDD174 pKa = 3.8 TALMSDD180 pKa = 4.88 MIDD183 pKa = 3.48 VIAPFLQPDD192 pKa = 3.96 AAALPCGDD200 pKa = 4.89 HH201 pKa = 5.97 FTMGPWQAAVAADD214 pKa = 3.92 WLGVDD219 pKa = 4.28 HH220 pKa = 6.79 VFPMHH225 pKa = 6.99 YY226 pKa = 8.89 DD227 pKa = 3.72 TFPPIEE233 pKa = 5.09 IDD235 pKa = 3.47 VQDD238 pKa = 4.28 FVDD241 pKa = 4.18 EE242 pKa = 4.34 VEE244 pKa = 4.4 KK245 pKa = 10.45 TGTDD249 pKa = 3.23 ADD251 pKa = 3.99 VHH253 pKa = 7.07 VLDD256 pKa = 4.99 GDD258 pKa = 3.57 EE259 pKa = 5.0 SYY261 pKa = 10.49 TLEE264 pKa = 4.06
Molecular weight: 28.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.808
IPC_protein 3.834
Toseland 3.617
ProMoST 3.986
Dawson 3.834
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.668
Grimsley 3.516
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.215
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.961
Patrickios 1.011
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.858
Protein with the highest isoelectric point:
>tr|A0A1H1AFW9|A0A1H1AFW9_9EURY Winged helix-turn-helix DNA-binding OS=Halopelagius longus OX=1236180 GN=DWB78_00745 PE=4 SV=1
MM1 pKa = 7.22 NVLFAFQIKK10 pKa = 10.49 LMFPLFLRR18 pKa = 11.84 LLLVNKK24 pKa = 9.99 SDD26 pKa = 3.8 SLPWSLFLLIILLVRR41 pKa = 11.84 PCTFRR46 pKa = 11.84 LKK48 pKa = 10.56 FIQVSILQSSLLHH61 pKa = 6.11 GGVVQGTVRR70 pKa = 11.84 SLTALLPGINVRR82 pKa = 11.84 VLPVYY87 pKa = 10.4 IVDD90 pKa = 3.7 DD91 pKa = 4.08 HH92 pKa = 6.75 LL93 pKa = 6.35
Molecular weight: 10.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.589
IPC_protein 10.189
Toseland 10.672
ProMoST 10.277
Dawson 10.745
Bjellqvist 10.423
Wikipedia 10.921
Rodwell 11.052
Grimsley 10.789
Solomon 10.862
Lehninger 10.847
Nozaki 10.657
DTASelect 10.409
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.687
Patrickios 10.906
IPC_peptide 10.862
IPC2_peptide 9.428
IPC2.peptide.svr19 8.581
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3802
0
3802
1114234
29
1805
293.1
31.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.865 ± 0.058
0.669 ± 0.013
7.997 ± 0.049
8.998 ± 0.065
3.468 ± 0.024
8.645 ± 0.036
1.854 ± 0.02
3.539 ± 0.032
1.966 ± 0.027
8.702 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.775 ± 0.018
2.442 ± 0.025
4.642 ± 0.023
2.194 ± 0.024
6.913 ± 0.048
5.814 ± 0.033
6.168 ± 0.035
9.462 ± 0.048
1.149 ± 0.015
2.738 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here