Halopelagius longus

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Halopelagius

Average proteome isoelectric point is 4.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3802 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1H1BB56|A0A1H1BB56_9EURY Sugar or nucleoside kinase ribokinase family OS=Halopelagius longus OX=1236180 GN=DWB78_02675 PE=4 SV=1
MM1 pKa = 7.35SIVVGGAAFGIFYY14 pKa = 8.23GTVVSSSRR22 pKa = 11.84RR23 pKa = 11.84LMTMEE28 pKa = 4.33LTWHH32 pKa = 6.17GHH34 pKa = 4.56STWHH38 pKa = 6.21VDD40 pKa = 3.02VDD42 pKa = 3.79GTTFLIDD49 pKa = 3.54PFFDD53 pKa = 3.79NPHH56 pKa = 6.37TDD58 pKa = 4.01LEE60 pKa = 4.64PEE62 pKa = 4.13DD63 pKa = 5.25VEE65 pKa = 4.58TPDD68 pKa = 4.52YY69 pKa = 11.42LLLTHH74 pKa = 6.38GHH76 pKa = 7.17ADD78 pKa = 3.89HH79 pKa = 6.89IADD82 pKa = 3.26VDD84 pKa = 3.9AFTDD88 pKa = 3.51ATLVAVPEE96 pKa = 4.35MTAFMQDD103 pKa = 2.64EE104 pKa = 4.82HH105 pKa = 8.35GFEE108 pKa = 4.69DD109 pKa = 5.39AIGMNLGGTTEE120 pKa = 4.48CGDD123 pKa = 3.89AFVTMHH129 pKa = 7.22RR130 pKa = 11.84ADD132 pKa = 3.55HH133 pKa = 6.09TNGLNTSYY141 pKa = 10.48EE142 pKa = 4.05NEE144 pKa = 3.99IGVPAGFVISDD155 pKa = 3.54EE156 pKa = 4.62RR157 pKa = 11.84PSQDD161 pKa = 3.39EE162 pKa = 4.38DD163 pKa = 3.89DD164 pKa = 4.48GATAFYY170 pKa = 10.37HH171 pKa = 6.94AGDD174 pKa = 3.8TALMSDD180 pKa = 4.88MIDD183 pKa = 3.48VIAPFLQPDD192 pKa = 3.96AAALPCGDD200 pKa = 4.89HH201 pKa = 5.97FTMGPWQAAVAADD214 pKa = 3.92WLGVDD219 pKa = 4.28HH220 pKa = 6.79VFPMHH225 pKa = 6.99YY226 pKa = 8.89DD227 pKa = 3.72TFPPIEE233 pKa = 5.09IDD235 pKa = 3.47VQDD238 pKa = 4.28FVDD241 pKa = 4.18EE242 pKa = 4.34VEE244 pKa = 4.4KK245 pKa = 10.45TGTDD249 pKa = 3.23ADD251 pKa = 3.99VHH253 pKa = 7.07VLDD256 pKa = 4.99GDD258 pKa = 3.57EE259 pKa = 5.0SYY261 pKa = 10.49TLEE264 pKa = 4.06

Molecular weight:
28.8 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1H1AFW9|A0A1H1AFW9_9EURY Winged helix-turn-helix DNA-binding OS=Halopelagius longus OX=1236180 GN=DWB78_00745 PE=4 SV=1
MM1 pKa = 7.22NVLFAFQIKK10 pKa = 10.49LMFPLFLRR18 pKa = 11.84LLLVNKK24 pKa = 9.99SDD26 pKa = 3.8SLPWSLFLLIILLVRR41 pKa = 11.84PCTFRR46 pKa = 11.84LKK48 pKa = 10.56FIQVSILQSSLLHH61 pKa = 6.11GGVVQGTVRR70 pKa = 11.84SLTALLPGINVRR82 pKa = 11.84VLPVYY87 pKa = 10.4IVDD90 pKa = 3.7DD91 pKa = 4.08HH92 pKa = 6.75LL93 pKa = 6.35

Molecular weight:
10.54 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3802

0

3802

1114234

29

1805

293.1

31.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.865 ± 0.058

0.669 ± 0.013

7.997 ± 0.049

8.998 ± 0.065

3.468 ± 0.024

8.645 ± 0.036

1.854 ± 0.02

3.539 ± 0.032

1.966 ± 0.027

8.702 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.775 ± 0.018

2.442 ± 0.025

4.642 ± 0.023

2.194 ± 0.024

6.913 ± 0.048

5.814 ± 0.033

6.168 ± 0.035

9.462 ± 0.048

1.149 ± 0.015

2.738 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski