Whitefly associated Puerto Rico alphasatellite 1

Taxonomy: Viruses; Alphasatellitidae; Geminialphasatellitinae; Clecrusatellite

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X9EGW1|A0A0X9EGW1_9VIRU Replication initiator protein OS=Whitefly associated Puerto Rico alphasatellite 1 OX=2169829 PE=4 SV=1
MM1 pKa = 7.39GKK3 pKa = 9.55SRR5 pKa = 11.84NYY7 pKa = 10.35CFTLFSYY14 pKa = 9.55VLPLFSSLPEE24 pKa = 3.66WANYY28 pKa = 10.27LVFQEE33 pKa = 4.78EE34 pKa = 4.64EE35 pKa = 4.3SPSTGRR41 pKa = 11.84KK42 pKa = 9.03HH43 pKa = 4.85IQGYY47 pKa = 10.27VNLKK51 pKa = 9.7NPQSFSFLKK60 pKa = 10.5KK61 pKa = 9.76KK62 pKa = 10.78LGDD65 pKa = 3.86GVHH68 pKa = 6.74LEE70 pKa = 4.16QARR73 pKa = 11.84GTASSNRR80 pKa = 11.84DD81 pKa = 3.23YY82 pKa = 10.56CTKK85 pKa = 10.51NDD87 pKa = 3.67SRR89 pKa = 11.84LSGPWEE95 pKa = 4.03FGVLAEE101 pKa = 4.38QGSKK105 pKa = 9.94KK106 pKa = 10.06RR107 pKa = 11.84KK108 pKa = 7.07TMEE111 pKa = 4.01SFQEE115 pKa = 4.17DD116 pKa = 4.27PEE118 pKa = 4.18EE119 pKa = 4.99LRR121 pKa = 11.84LSDD124 pKa = 3.31PKK126 pKa = 10.91LYY128 pKa = 10.27RR129 pKa = 11.84RR130 pKa = 11.84CLATRR135 pKa = 11.84VNTEE139 pKa = 3.34FSGLVLPVLDD149 pKa = 5.35RR150 pKa = 11.84PWQLLVEE157 pKa = 4.47KK158 pKa = 10.54VLDD161 pKa = 4.18EE162 pKa = 4.9GPDD165 pKa = 3.38DD166 pKa = 3.88RR167 pKa = 11.84TIIWVYY173 pKa = 10.56GSQGNEE179 pKa = 4.34GKK181 pKa = 7.1TTWAKK186 pKa = 10.81SKK188 pKa = 10.64VQAGWFYY195 pKa = 11.16SRR197 pKa = 11.84GGKK200 pKa = 9.86GEE202 pKa = 4.16NIKK205 pKa = 10.8YY206 pKa = 10.46SYY208 pKa = 11.0AEE210 pKa = 4.1HH211 pKa = 6.89LGHH214 pKa = 7.37AVFDD218 pKa = 4.21LPRR221 pKa = 11.84QVEE224 pKa = 4.37DD225 pKa = 3.23VLQYY229 pKa = 10.56TVLEE233 pKa = 4.5EE234 pKa = 3.95IKK236 pKa = 10.78DD237 pKa = 3.69RR238 pKa = 11.84LIRR241 pKa = 11.84SSKK244 pKa = 10.56YY245 pKa = 10.02EE246 pKa = 4.51PIDD249 pKa = 3.92FNCSDD254 pKa = 3.5QVHH257 pKa = 5.25VVVLSNFLPQLDD269 pKa = 4.38IEE271 pKa = 4.84HH272 pKa = 7.33DD273 pKa = 3.99SKK275 pKa = 12.11GNLLKK280 pKa = 10.77KK281 pKa = 10.53QMLSRR286 pKa = 11.84DD287 pKa = 2.9RR288 pKa = 11.84VVIVNIDD295 pKa = 3.4EE296 pKa = 4.44SVLVRR301 pKa = 11.84DD302 pKa = 4.07HH303 pKa = 6.09EE304 pKa = 4.7TVTFHH309 pKa = 7.53EE310 pKa = 4.5YY311 pKa = 9.5MKK313 pKa = 11.05

Molecular weight: 36.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0X9EGW1|A0A0X9EGW1_9VIRU Replication initiator protein OS=Whitefly associated Puerto Rico alphasatellite 1 OX=2169829 PE=4 SV=1
MM1 pKa = 7.39GKK3 pKa = 9.55SRR5 pKa = 11.84NYY7 pKa = 10.35CFTLFSYY14 pKa = 9.55VLPLFSSLPEE24 pKa = 3.66WANYY28 pKa = 10.27LVFQEE33 pKa = 4.78EE34 pKa = 4.64EE35 pKa = 4.3SPSTGRR41 pKa = 11.84KK42 pKa = 9.03HH43 pKa = 4.85IQGYY47 pKa = 10.27VNLKK51 pKa = 9.7NPQSFSFLKK60 pKa = 10.5KK61 pKa = 9.76KK62 pKa = 10.78LGDD65 pKa = 3.86GVHH68 pKa = 6.74LEE70 pKa = 4.16QARR73 pKa = 11.84GTASSNRR80 pKa = 11.84DD81 pKa = 3.23YY82 pKa = 10.56CTKK85 pKa = 10.51NDD87 pKa = 3.67SRR89 pKa = 11.84LSGPWEE95 pKa = 4.03FGVLAEE101 pKa = 4.38QGSKK105 pKa = 9.94KK106 pKa = 10.06RR107 pKa = 11.84KK108 pKa = 7.07TMEE111 pKa = 4.01SFQEE115 pKa = 4.17DD116 pKa = 4.27PEE118 pKa = 4.18EE119 pKa = 4.99LRR121 pKa = 11.84LSDD124 pKa = 3.31PKK126 pKa = 10.91LYY128 pKa = 10.27RR129 pKa = 11.84RR130 pKa = 11.84CLATRR135 pKa = 11.84VNTEE139 pKa = 3.34FSGLVLPVLDD149 pKa = 5.35RR150 pKa = 11.84PWQLLVEE157 pKa = 4.47KK158 pKa = 10.54VLDD161 pKa = 4.18EE162 pKa = 4.9GPDD165 pKa = 3.38DD166 pKa = 3.88RR167 pKa = 11.84TIIWVYY173 pKa = 10.56GSQGNEE179 pKa = 4.34GKK181 pKa = 7.1TTWAKK186 pKa = 10.81SKK188 pKa = 10.64VQAGWFYY195 pKa = 11.16SRR197 pKa = 11.84GGKK200 pKa = 9.86GEE202 pKa = 4.16NIKK205 pKa = 10.8YY206 pKa = 10.46SYY208 pKa = 11.0AEE210 pKa = 4.1HH211 pKa = 6.89LGHH214 pKa = 7.37AVFDD218 pKa = 4.21LPRR221 pKa = 11.84QVEE224 pKa = 4.37DD225 pKa = 3.23VLQYY229 pKa = 10.56TVLEE233 pKa = 4.5EE234 pKa = 3.95IKK236 pKa = 10.78DD237 pKa = 3.69RR238 pKa = 11.84LIRR241 pKa = 11.84SSKK244 pKa = 10.56YY245 pKa = 10.02EE246 pKa = 4.51PIDD249 pKa = 3.92FNCSDD254 pKa = 3.5QVHH257 pKa = 5.25VVVLSNFLPQLDD269 pKa = 4.38IEE271 pKa = 4.84HH272 pKa = 7.33DD273 pKa = 3.99SKK275 pKa = 12.11GNLLKK280 pKa = 10.77KK281 pKa = 10.53QMLSRR286 pKa = 11.84DD287 pKa = 2.9RR288 pKa = 11.84VVIVNIDD295 pKa = 3.4EE296 pKa = 4.44SVLVRR301 pKa = 11.84DD302 pKa = 4.07HH303 pKa = 6.09EE304 pKa = 4.7TVTFHH309 pKa = 7.53EE310 pKa = 4.5YY311 pKa = 9.5MKK313 pKa = 11.05

Molecular weight: 36.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

313

313

313

313.0

36.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.875 ± 0.0

1.278 ± 0.0

6.07 ± 0.0

7.987 ± 0.0

4.473 ± 0.0

6.39 ± 0.0

2.556 ± 0.0

3.195 ± 0.0

7.348 ± 0.0

10.543 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.278 ± 0.0

4.153 ± 0.0

4.153 ± 0.0

4.473 ± 0.0

6.07 ± 0.0

8.626 ± 0.0

4.153 ± 0.0

8.307 ± 0.0

1.917 ± 0.0

4.153 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski