Streptococcus phage Javan117

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZY49|A0A4D5ZY49_9CAUD Tail length tape-measure protein OS=Streptococcus phage Javan117 OX=2547986 GN=Javan117_0021 PE=4 SV=1
MM1 pKa = 7.13ATVSEE6 pKa = 4.8VISYY10 pKa = 8.69WRR12 pKa = 11.84GLADD16 pKa = 3.36TGQGYY21 pKa = 9.04DD22 pKa = 5.26ADD24 pKa = 5.1LAWGWQCADD33 pKa = 3.49VTNGTTTNFFGVTLWGNAIDD53 pKa = 5.23LLDD56 pKa = 3.77SAKK59 pKa = 10.87AQGLEE64 pKa = 4.26VIYY67 pKa = 9.61DD68 pKa = 3.63APGINPKK75 pKa = 10.66AGDD78 pKa = 3.99LFVMFTYY85 pKa = 9.14GHH87 pKa = 7.42PYY89 pKa = 8.44GHH91 pKa = 6.79TGIVIADD98 pKa = 3.46SDD100 pKa = 4.66GYY102 pKa = 9.33TIQTIEE108 pKa = 4.14QNVDD112 pKa = 2.94GYY114 pKa = 11.54SDD116 pKa = 3.84NNGDD120 pKa = 4.17GINDD124 pKa = 3.52QFQVGGPARR133 pKa = 11.84YY134 pKa = 7.58VTRR137 pKa = 11.84AFSDD141 pKa = 3.37GDD143 pKa = 3.96GYY145 pKa = 10.51IVGWIRR151 pKa = 11.84PPYY154 pKa = 10.1SDD156 pKa = 5.21APSEE160 pKa = 4.19QQSQPQAPSGFRR172 pKa = 11.84KK173 pKa = 10.24LKK175 pKa = 10.89DD176 pKa = 3.35EE177 pKa = 4.36VGTFEE182 pKa = 5.09VMVPALNVRR191 pKa = 11.84RR192 pKa = 11.84EE193 pKa = 3.77PSLNGEE199 pKa = 3.97IVACYY204 pKa = 9.54QYY206 pKa = 11.74GMTGTYY212 pKa = 10.62DD213 pKa = 3.12SVYY216 pKa = 11.08VGDD219 pKa = 4.08GYY221 pKa = 10.78IWVSYY226 pKa = 10.44VGASGMRR233 pKa = 11.84NYY235 pKa = 9.76MAVGDD240 pKa = 4.48ADD242 pKa = 3.22GDD244 pKa = 4.55YY245 pKa = 11.31NVNPYY250 pKa = 10.72CKK252 pKa = 10.02FYY254 pKa = 11.49

Molecular weight: 27.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZYX0|A0A4D5ZYX0_9CAUD Cytosine-specific methyltransferase OS=Streptococcus phage Javan117 OX=2547986 GN=Javan117_0040 PE=3 SV=1
MM1 pKa = 7.55NKK3 pKa = 10.01RR4 pKa = 11.84IKK6 pKa = 10.17KK7 pKa = 9.36KK8 pKa = 10.28RR9 pKa = 11.84RR10 pKa = 11.84LEE12 pKa = 4.1TLLGLAIGQITALRR26 pKa = 11.84LEE28 pKa = 4.32LDD30 pKa = 3.58AVKK33 pKa = 10.54SATLDD38 pKa = 3.27NQVAIKK44 pKa = 10.65SIGDD48 pKa = 4.03DD49 pKa = 2.85IDD51 pKa = 4.32YY52 pKa = 10.83IKK54 pKa = 10.85QNYY57 pKa = 7.33KK58 pKa = 10.49RR59 pKa = 11.84KK60 pKa = 8.22WRR62 pKa = 11.84KK63 pKa = 8.88

Molecular weight: 7.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11787

40

1147

203.2

22.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.787 ± 0.395

0.781 ± 0.141

6.719 ± 0.315

6.965 ± 0.336

3.758 ± 0.186

6.702 ± 0.388

1.239 ± 0.101

6.821 ± 0.215

8.9 ± 0.415

7.831 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.062 ± 0.125

6.058 ± 0.229

2.63 ± 0.173

3.758 ± 0.183

3.58 ± 0.22

6.499 ± 0.37

6.634 ± 0.323

6.745 ± 0.232

1.383 ± 0.137

4.149 ± 0.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski