Candidatus Nitrosocosmicus arcticus

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Nitrososphaeria; Nitrososphaerales; Nitrososphaeraceae; Candidatus Nitrosocosmicus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3069 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A557SVZ9|A0A557SVZ9_9ARCH Putative ester cyclase OS=Candidatus Nitrosocosmicus arcticus OX=2035267 GN=NARC_60163 PE=4 SV=1
MM1 pKa = 9.01VMIKK5 pKa = 10.39YY6 pKa = 9.81VLIPIFVVITLFTTGLVEE24 pKa = 4.07NSFSVEE30 pKa = 3.72LRR32 pKa = 11.84DD33 pKa = 4.09LKK35 pKa = 10.41TIISNDD41 pKa = 3.38EE42 pKa = 3.74ATKK45 pKa = 10.24EE46 pKa = 3.9YY47 pKa = 11.07AVIPNQEE54 pKa = 4.47LLVIPLTDD62 pKa = 3.39INSIAYY68 pKa = 8.32YY69 pKa = 10.05QLNTFDD75 pKa = 4.39QSYY78 pKa = 8.13TFQNVTVLEE87 pKa = 4.34GVVYY91 pKa = 10.35EE92 pKa = 4.74DD93 pKa = 4.72KK94 pKa = 11.06KK95 pKa = 11.28SNNDD99 pKa = 3.09NGATSGDD106 pKa = 4.08LDD108 pKa = 4.52CSDD111 pKa = 3.15VAARR115 pKa = 11.84NFLVGSSDD123 pKa = 3.31SYY125 pKa = 11.95NFDD128 pKa = 3.59RR129 pKa = 11.84DD130 pKa = 3.29GDD132 pKa = 4.39GIGCEE137 pKa = 3.89SSNYY141 pKa = 8.78YY142 pKa = 10.59NDD144 pKa = 3.66YY145 pKa = 10.52NSLYY149 pKa = 11.03AVTSDD154 pKa = 3.49NNSRR158 pKa = 11.84CPDD161 pKa = 3.31GFHH164 pKa = 7.33RR165 pKa = 11.84SPSGDD170 pKa = 3.56CEE172 pKa = 4.0RR173 pKa = 11.84VTDD176 pKa = 3.68TSGMEE181 pKa = 3.74RR182 pKa = 11.84CPDD185 pKa = 3.33GFHH188 pKa = 7.27RR189 pKa = 11.84SPSGDD194 pKa = 3.52CEE196 pKa = 3.84RR197 pKa = 11.84VTDD200 pKa = 5.11NNDD203 pKa = 3.05NDD205 pKa = 4.45KK206 pKa = 11.29GSNPCTPIEE215 pKa = 3.96ASKK218 pKa = 11.32DD219 pKa = 3.47HH220 pKa = 6.65TCTSGSEE227 pKa = 4.26GIDD230 pKa = 3.15YY231 pKa = 10.28SKK233 pKa = 11.17PNNNDD238 pKa = 2.79NDD240 pKa = 3.91ASNNDD245 pKa = 3.58YY246 pKa = 11.7DD247 pKa = 6.23NIDD250 pKa = 3.39NWTDD254 pKa = 3.5PPAEE258 pKa = 4.19EE259 pKa = 4.19TTEE262 pKa = 4.23GNGVEE267 pKa = 4.29NEE269 pKa = 3.96EE270 pKa = 5.35SGQQEE275 pKa = 4.14NNEE278 pKa = 4.07NTEE281 pKa = 4.0EE282 pKa = 4.23QPNNDD287 pKa = 4.08EE288 pKa = 4.9GSSSSDD294 pKa = 3.27EE295 pKa = 4.41GSSSSDD301 pKa = 3.27EE302 pKa = 4.41GSSSSDD308 pKa = 3.13EE309 pKa = 4.12GSSDD313 pKa = 3.61NNN315 pKa = 3.5

Molecular weight: 34.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A557STA8|A0A557STA8_9ARCH Methylenetetrahydrofolate reductase (NAD(P)H) OS=Candidatus Nitrosocosmicus arcticus OX=2035267 GN=metF PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 9.86RR3 pKa = 11.84APPPPPLTPLTAGMKK18 pKa = 9.71RR19 pKa = 11.84APPPPPLTPLTAGMKK34 pKa = 9.95KK35 pKa = 10.2ALPLMSLLRR44 pKa = 11.84HH45 pKa = 6.14PNLLIQLAQIRR56 pKa = 11.84RR57 pKa = 11.84KK58 pKa = 8.79LTTWRR63 pKa = 11.84KK64 pKa = 8.7KK65 pKa = 9.72IRR67 pKa = 11.84AISINKK73 pKa = 9.4LVDD76 pKa = 3.09NAFFANTGIFGTKK89 pKa = 9.82NNSKK93 pKa = 9.42MPRR96 pKa = 11.84PTNIKK101 pKa = 10.13VNSS104 pKa = 3.69

Molecular weight: 11.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3069

0

3069

668652

28

1529

217.9

24.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.961 ± 0.053

1.049 ± 0.022

5.726 ± 0.04

6.215 ± 0.045

4.571 ± 0.037

6.054 ± 0.052

1.806 ± 0.021

9.62 ± 0.055

7.598 ± 0.049

9.121 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.024

6.635 ± 0.055

3.624 ± 0.038

3.004 ± 0.033

3.734 ± 0.037

8.03 ± 0.046

5.442 ± 0.046

5.94 ± 0.039

0.844 ± 0.016

3.564 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski