Lactobacillus virus LLKu
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F7V9E2|F7V9E2_9CAUD Uncharacterized protein OS=Lactobacillus virus LLKu OX=297254 PE=4 SV=1
MM1 pKa = 7.29 KK2 pKa = 10.35 VISFNEE8 pKa = 4.21 CNRR11 pKa = 11.84 GSAYY15 pKa = 9.86 TYY17 pKa = 10.42 IGFTNSSITGDD28 pKa = 3.43 EE29 pKa = 4.35 DD30 pKa = 4.59 YY31 pKa = 9.96 EE32 pKa = 4.56 TVINRR37 pKa = 11.84 LVSATKK43 pKa = 10.63 YY44 pKa = 8.52 MQLHH48 pKa = 6.2 SGGAPVLIAGDD59 pKa = 3.63 KK60 pKa = 10.45 VEE62 pKa = 4.26 VVGDD66 pKa = 4.2 LEE68 pKa = 5.65 DD69 pKa = 3.56 EE70 pKa = 4.32
Molecular weight: 7.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.275
IPC2_protein 4.431
IPC_protein 4.253
Toseland 4.088
ProMoST 4.291
Dawson 4.215
Bjellqvist 4.457
Wikipedia 4.113
Rodwell 4.101
Grimsley 4.012
Solomon 4.202
Lehninger 4.151
Nozaki 4.342
DTASelect 4.482
Thurlkill 4.126
EMBOSS 4.126
Sillero 4.368
Patrickios 3.16
IPC_peptide 4.202
IPC2_peptide 4.355
IPC2.peptide.svr19 4.303
Protein with the highest isoelectric point:
>tr|F7V9A4|F7V9A4_9CAUD Putative portal protein OS=Lactobacillus virus LLKu OX=297254 PE=4 SV=1
MM1 pKa = 7.32 NSEE4 pKa = 3.87 NYY6 pKa = 9.47 KK7 pKa = 10.07 VYY9 pKa = 11.0 KK10 pKa = 9.22 HH11 pKa = 5.26 TTPNNKK17 pKa = 9.35 VYY19 pKa = 10.57 IGITKK24 pKa = 10.27 LDD26 pKa = 3.52 VNKK29 pKa = 10.17 RR30 pKa = 11.84 WKK32 pKa = 10.28 NGNGYY37 pKa = 9.33 KK38 pKa = 10.14 HH39 pKa = 5.82 NQYY42 pKa = 8.26 FTRR45 pKa = 11.84 AII47 pKa = 3.61
Molecular weight: 5.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.161
IPC2_protein 9.765
IPC_protein 9.736
Toseland 10.116
ProMoST 9.823
Dawson 10.335
Bjellqvist 9.999
Wikipedia 10.511
Rodwell 10.862
Grimsley 10.409
Solomon 10.35
Lehninger 10.321
Nozaki 10.072
DTASelect 9.999
Thurlkill 10.16
EMBOSS 10.511
Sillero 10.233
Patrickios 10.599
IPC_peptide 10.35
IPC2_peptide 8.595
IPC2.peptide.svr19 8.663
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
9498
44
968
186.2
21.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.538 ± 0.525
0.979 ± 0.175
6.422 ± 0.359
6.875 ± 0.54
3.864 ± 0.206
6.686 ± 0.378
1.39 ± 0.175
6.865 ± 0.309
8.475 ± 0.466
7.654 ± 0.283
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.537 ± 0.205
6.728 ± 0.328
2.453 ± 0.172
3.39 ± 0.257
4.18 ± 0.298
6.58 ± 0.343
5.97 ± 0.309
7.023 ± 0.173
1.021 ± 0.125
4.369 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here