Firmicutes bacterium CAG:103

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1809 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5B8H8|R5B8H8_9FIRM Orotidine 5'-phosphate decarboxylase OS=Firmicutes bacterium CAG:103 OX=1262999 GN=pyrF PE=3 SV=1
MM1 pKa = 7.77PAPEE5 pKa = 4.73APQGQGDD12 pKa = 4.42WYY14 pKa = 10.89APAPGGGVSSPEE26 pKa = 3.72IGPKK30 pKa = 10.06HH31 pKa = 6.23KK32 pKa = 10.11PKK34 pKa = 10.56KK35 pKa = 10.35LLIGLLCGFAALVVIVAVLAGIFAARR61 pKa = 11.84QAGEE65 pKa = 3.87PSTAGGAEE73 pKa = 3.92VDD75 pKa = 3.99RR76 pKa = 11.84LLEE79 pKa = 4.22RR80 pKa = 11.84YY81 pKa = 7.48FTYY84 pKa = 10.32FKK86 pKa = 11.21ADD88 pKa = 3.59DD89 pKa = 3.89TDD91 pKa = 4.32AMLDD95 pKa = 3.53LFLPEE100 pKa = 4.79VITYY104 pKa = 7.74MQDD107 pKa = 3.07DD108 pKa = 3.82DD109 pKa = 4.02TAAGRR114 pKa = 11.84EE115 pKa = 3.89AALIQLDD122 pKa = 3.67DD123 pKa = 3.37WYY125 pKa = 11.17YY126 pKa = 11.44DD127 pKa = 3.42YY128 pKa = 11.83GKK130 pKa = 10.59AIDD133 pKa = 4.97DD134 pKa = 4.29YY135 pKa = 11.61YY136 pKa = 11.83VYY138 pKa = 11.19DD139 pKa = 5.51DD140 pKa = 5.34DD141 pKa = 6.29SLDD144 pKa = 3.69ASDD147 pKa = 3.99VRR149 pKa = 11.84EE150 pKa = 4.1LQRR153 pKa = 11.84EE154 pKa = 4.07LGVRR158 pKa = 11.84IDD160 pKa = 4.21DD161 pKa = 4.07NVIVACDD168 pKa = 3.29VTYY171 pKa = 11.18QNGQDD176 pKa = 2.98NDD178 pKa = 4.05FYY180 pKa = 11.65FEE182 pKa = 4.13FVQVDD187 pKa = 4.12GQWYY191 pKa = 9.39LLFVVEE197 pKa = 4.69

Molecular weight: 21.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5AXB9|R5AXB9_9FIRM 2 3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Firmicutes bacterium CAG:103 OX=1262999 GN=gpmI PE=3 SV=1
MM1 pKa = 7.67PPLRR5 pKa = 11.84KK6 pKa = 9.44IICRR10 pKa = 11.84CWRR13 pKa = 11.84TTSSTTSLSISRR25 pKa = 11.84STIRR29 pKa = 11.84RR30 pKa = 11.84PAWRR34 pKa = 11.84SSTKK38 pKa = 10.01CRR40 pKa = 11.84TSRR43 pKa = 11.84RR44 pKa = 11.84PSRR47 pKa = 11.84RR48 pKa = 11.84QPRR51 pKa = 11.84PRR53 pKa = 11.84RR54 pKa = 11.84ALRR57 pKa = 11.84PRR59 pKa = 11.84AKK61 pKa = 9.95PHH63 pKa = 5.67PQSSRR68 pKa = 11.84SRR70 pKa = 11.84LPSRR74 pKa = 11.84RR75 pKa = 11.84SSSTSRR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84TTSRR87 pKa = 11.84TSRR90 pKa = 11.84VRR92 pKa = 11.84WPSAASSIPAVRR104 pKa = 11.84RR105 pKa = 11.84RR106 pKa = 11.84TCPSTTSVWTISCPSARR123 pKa = 11.84RR124 pKa = 11.84ISVAASRR131 pKa = 11.84RR132 pKa = 11.84SRR134 pKa = 11.84TATVSVRR141 pKa = 11.84RR142 pKa = 11.84ILPPAPSAARR152 pKa = 11.84RR153 pKa = 11.84SATRR157 pKa = 11.84CRR159 pKa = 11.84SCSWRR164 pKa = 11.84SQKK167 pKa = 10.61SSSSRR172 pKa = 11.84SRR174 pKa = 11.84SRR176 pKa = 11.84TRR178 pKa = 11.84SPSASSPPAA187 pKa = 4.35

Molecular weight: 21.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1809

0

1809

526350

30

2120

291.0

31.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.954 ± 0.08

1.875 ± 0.029

5.939 ± 0.052

5.814 ± 0.059

3.647 ± 0.04

7.829 ± 0.062

2.056 ± 0.033

5.604 ± 0.054

4.201 ± 0.058

9.674 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.82 ± 0.03

3.064 ± 0.035

4.252 ± 0.037

3.311 ± 0.036

6.108 ± 0.075

5.087 ± 0.051

5.867 ± 0.061

7.475 ± 0.043

0.989 ± 0.025

3.41 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski