methanogenic archaeon ISO4-H5

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; unclassified Thermoplasmata

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1805 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X8UZ55|A0A0X8UZ55_9ARCH Uncharacterized protein OS=methanogenic archaeon ISO4-H5 OX=1495144 GN=AR505_1103 PE=4 SV=1
MM1 pKa = 7.3MGFTLSRR8 pKa = 11.84IALFACGAILMAAVAVPISEE28 pKa = 4.47MYY30 pKa = 9.85QDD32 pKa = 4.35RR33 pKa = 11.84GNDD36 pKa = 3.67SIEE39 pKa = 4.13KK40 pKa = 9.76VAEE43 pKa = 4.02ADD45 pKa = 3.68AMFVDD50 pKa = 4.69ALWDD54 pKa = 3.17IDD56 pKa = 3.65MDD58 pKa = 3.97EE59 pKa = 4.1TALRR63 pKa = 11.84GDD65 pKa = 3.68ILLPSPAYY73 pKa = 10.27GLIIDD78 pKa = 5.4GYY80 pKa = 10.34FLTISDD86 pKa = 4.94DD87 pKa = 4.01DD88 pKa = 4.56GNSYY92 pKa = 10.75VSSLKK97 pKa = 10.48HH98 pKa = 4.53RR99 pKa = 11.84TDD101 pKa = 4.25RR102 pKa = 11.84IEE104 pKa = 4.36LGYY107 pKa = 10.54GDD109 pKa = 4.16SVNVCRR115 pKa = 11.84YY116 pKa = 9.31GDD118 pKa = 3.66KK119 pKa = 11.23LMLCSNVPDD128 pKa = 4.72DD129 pKa = 4.57EE130 pKa = 4.92PVTEE134 pKa = 4.35TDD136 pKa = 4.8PLEE139 pKa = 4.79DD140 pKa = 3.66WDD142 pKa = 4.18TAPPEE147 pKa = 4.22EE148 pKa = 4.51PSAGNSYY155 pKa = 8.55TVTNEE160 pKa = 3.69SGNSITISCGPAA172 pKa = 2.78

Molecular weight: 18.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A110A296|A0A110A296_9ARCH Thiazole synthase OS=methanogenic archaeon ISO4-H5 OX=1495144 GN=AR505_0102 PE=4 SV=1
MM1 pKa = 7.44GAGTAAQGRR10 pKa = 11.84HH11 pKa = 5.4NKK13 pKa = 9.66HH14 pKa = 5.67KK15 pKa = 9.2THH17 pKa = 6.56IPCRR21 pKa = 11.84RR22 pKa = 11.84CGKK25 pKa = 9.77RR26 pKa = 11.84SYY28 pKa = 10.65SVRR31 pKa = 11.84KK32 pKa = 9.32GICASCGYY40 pKa = 10.42GKK42 pKa = 7.63TARR45 pKa = 11.84IRR47 pKa = 11.84TYY49 pKa = 8.52TWAKK53 pKa = 8.49VHH55 pKa = 5.91EE56 pKa = 4.42

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1805

0

1805

574731

29

3978

318.4

35.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.001 ± 0.075

1.667 ± 0.027

6.509 ± 0.042

6.697 ± 0.063

3.89 ± 0.036

7.724 ± 0.054

1.689 ± 0.022

6.896 ± 0.055

5.948 ± 0.059

8.265 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.201 ± 0.035

3.861 ± 0.042

3.891 ± 0.038

2.346 ± 0.024

4.946 ± 0.061

6.472 ± 0.074

5.811 ± 0.086

7.479 ± 0.051

0.952 ± 0.02

3.751 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski