Chimpanzee polyomavirus
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E5AX41|E5AX41_9POLY Minor capsid protein VP2 OS=Chimpanzee polyomavirus OX=305677 GN=VP3 PE=3 SV=1
MM1 pKa = 7.52 GGLLSSLVDD10 pKa = 4.06 MIVMASEE17 pKa = 4.57 LSAASGLTIEE27 pKa = 5.1 ALLTGEE33 pKa = 4.56 ALAALEE39 pKa = 4.51 AEE41 pKa = 4.95 VFSLMAVEE49 pKa = 4.35 GLSGIEE55 pKa = 4.47 ALAQLGWTAEE65 pKa = 4.19 QFSNMAFISTTFSNAIGYY83 pKa = 8.57 GVLFQTVSGISSLVSAGIRR102 pKa = 11.84 LGTSVSSVNRR112 pKa = 11.84 HH113 pKa = 3.58 QTEE116 pKa = 4.1 QEE118 pKa = 3.93 LEE120 pKa = 4.16 TLFGKK125 pKa = 9.98 IAHH128 pKa = 6.47 FLHH131 pKa = 6.58 VNLAFHH137 pKa = 7.44 LDD139 pKa = 3.46 PFDD142 pKa = 3.35 WCGSIGTTMPSEE154 pKa = 4.38 FSNLTLDD161 pKa = 3.85 QLSKK165 pKa = 10.81 LALIIEE171 pKa = 4.26 NGRR174 pKa = 11.84 WVVQRR179 pKa = 11.84 SPTHH183 pKa = 6.86 DD184 pKa = 3.82 PLFEE188 pKa = 6.25 SGDD191 pKa = 3.71 VIDD194 pKa = 5.03 IFGPPGGARR203 pKa = 11.84 QRR205 pKa = 11.84 VTPDD209 pKa = 2.19 WMLPLILRR217 pKa = 11.84 LNGAPEE223 pKa = 4.43 EE224 pKa = 4.2 KK225 pKa = 10.41 SSLCVNTNQSS235 pKa = 2.89
Molecular weight: 25.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.595
IPC2_protein 4.558
IPC_protein 4.457
Toseland 4.317
ProMoST 4.558
Dawson 4.393
Bjellqvist 4.546
Wikipedia 4.24
Rodwell 4.304
Grimsley 4.24
Solomon 4.393
Lehninger 4.342
Nozaki 4.507
DTASelect 4.622
Thurlkill 4.317
EMBOSS 4.266
Sillero 4.571
Patrickios 3.872
IPC_peptide 4.406
IPC2_peptide 4.571
IPC2.peptide.svr19 4.494
Protein with the highest isoelectric point:
>tr|E5AX40|E5AX40_9POLY Minor capsid protein VP2 OS=Chimpanzee polyomavirus OX=305677 GN=VP2 PE=3 SV=1
MM1 pKa = 6.66 FTCLGVKK8 pKa = 9.46 PRR10 pKa = 11.84 FRR12 pKa = 11.84 ACSQVIISNRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 TAACQRR31 pKa = 11.84 SFNWRR36 pKa = 11.84 KK37 pKa = 8.3 LTVCVRR43 pKa = 11.84 TVTCQANKK51 pKa = 10.48 SSGDD55 pKa = 3.34 QAGEE59 pKa = 4.06 NKK61 pKa = 10.42 LLLL64 pKa = 4.28
Molecular weight: 7.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.229
IPC2_protein 9.706
IPC_protein 10.965
Toseland 11.301
ProMoST 11.623
Dawson 11.316
Bjellqvist 11.213
Wikipedia 11.696
Rodwell 11.301
Grimsley 11.345
Solomon 11.711
Lehninger 11.623
Nozaki 11.301
DTASelect 11.199
Thurlkill 11.286
EMBOSS 11.769
Sillero 11.301
Patrickios 11.082
IPC_peptide 11.711
IPC2_peptide 10.716
IPC2.peptide.svr19 9.417
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2431
64
838
405.2
45.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.499 ± 0.318
2.633 ± 0.463
5.019 ± 0.388
6.417 ± 0.265
5.389 ± 0.794
6.006 ± 0.953
2.016 ± 0.094
5.677 ± 0.191
6.541 ± 1.057
10.942 ± 0.785
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.386 ± 0.196
4.525 ± 0.304
5.594 ± 0.997
4.237 ± 0.153
4.031 ± 0.392
8.186 ± 0.921
5.306 ± 0.8
5.183 ± 0.396
1.193 ± 0.161
2.221 ± 0.404
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here