Epirus cherry virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Miaviricetes; Ourlivirales; Botourmiaviridae; Ourmiavirus

Average proteome isoelectric point is 8.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3VML0|B3VML0_9VIRU Coat protein OS=Epirus cherry virus OX=544686 PE=2 SV=1
MM1 pKa = 7.74EE2 pKa = 4.61PTGNNPTVNQPMPVVVAPSHH22 pKa = 5.73SGQMRR27 pKa = 11.84VDD29 pKa = 4.25LSSLQIRR36 pKa = 11.84PPPLEE41 pKa = 3.98GKK43 pKa = 10.16GRR45 pKa = 11.84VVFVTEE51 pKa = 4.09HH52 pKa = 5.45SAYY55 pKa = 10.66DD56 pKa = 3.69GVRR59 pKa = 11.84PLPLIPKK66 pKa = 9.7DD67 pKa = 3.28LAGQLRR73 pKa = 11.84LKK75 pKa = 9.24WHH77 pKa = 6.18EE78 pKa = 3.54KK79 pKa = 10.05RR80 pKa = 11.84YY81 pKa = 8.0PHH83 pKa = 6.99TYY85 pKa = 10.35LAFEE89 pKa = 4.43QIEE92 pKa = 4.57SEE94 pKa = 4.52YY95 pKa = 10.63FPHH98 pKa = 7.56IPEE101 pKa = 4.1RR102 pKa = 11.84SAYY105 pKa = 10.25GEE107 pKa = 4.01LALVDD112 pKa = 3.38TRR114 pKa = 11.84YY115 pKa = 8.59ITDD118 pKa = 3.35EE119 pKa = 4.03VQDD122 pKa = 3.54VDD124 pKa = 3.94EE125 pKa = 5.35EE126 pKa = 4.26EE127 pKa = 4.74MIDD130 pKa = 3.7HH131 pKa = 6.97LFSRR135 pKa = 11.84ALWKK139 pKa = 9.48TPEE142 pKa = 4.26LDD144 pKa = 3.1LGKK147 pKa = 10.62GYY149 pKa = 10.76KK150 pKa = 9.39FVSAVPYY157 pKa = 8.92CIPIHH162 pKa = 6.27ARR164 pKa = 11.84GGQGPDD170 pKa = 3.37LEE172 pKa = 4.66RR173 pKa = 11.84DD174 pKa = 3.02IPIRR178 pKa = 11.84LVPRR182 pKa = 11.84ITRR185 pKa = 11.84TDD187 pKa = 2.9LHH189 pKa = 6.2LASRR193 pKa = 11.84SGTIKK198 pKa = 10.56SALKK202 pKa = 10.1VALSSEE208 pKa = 4.65PIQYY212 pKa = 9.9QRR214 pKa = 11.84IEE216 pKa = 4.37SVSAQTLEE224 pKa = 4.05ARR226 pKa = 11.84NSQITARR233 pKa = 11.84GRR235 pKa = 11.84RR236 pKa = 11.84NLDD239 pKa = 2.87RR240 pKa = 11.84RR241 pKa = 11.84PRR243 pKa = 11.84GGPWPAMEE251 pKa = 5.29RR252 pKa = 11.84SHH254 pKa = 8.0SMRR257 pKa = 11.84SDD259 pKa = 3.16TQIPIEE265 pKa = 4.27HH266 pKa = 7.23AAPPGGGEE274 pKa = 3.96PSRR277 pKa = 11.84TIIMPAGAVV286 pKa = 3.24

Molecular weight: 31.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3VML0|B3VML0_9VIRU Coat protein OS=Epirus cherry virus OX=544686 PE=2 SV=1
MM1 pKa = 6.92VNRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84GIRR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84NRR15 pKa = 11.84RR16 pKa = 11.84QGTNQQAPIPRR27 pKa = 11.84ISGVRR32 pKa = 11.84SYY34 pKa = 11.24DD35 pKa = 3.27VEE37 pKa = 4.21VFRR40 pKa = 11.84DD41 pKa = 4.22FTFLNRR47 pKa = 11.84RR48 pKa = 11.84NGCAVGQLGPGTGVTQISGLIIPDD72 pKa = 3.72TAISWNIKK80 pKa = 8.58TMSLNPINLVPSTVGGQFLISLVPGPALSGRR111 pKa = 11.84VLADD115 pKa = 3.61LQAIQTGSLMSSPSTLRR132 pKa = 11.84IFPARR137 pKa = 11.84PIPGQPWRR145 pKa = 11.84GSLIQRR151 pKa = 11.84EE152 pKa = 4.45ITRR155 pKa = 11.84SNDD158 pKa = 2.87SRR160 pKa = 11.84VVNPGDD166 pKa = 3.07RR167 pKa = 11.84WGGFAIVSDD176 pKa = 3.76SRR178 pKa = 11.84LLSGLADD185 pKa = 4.42DD186 pKa = 4.77APVWFARR193 pKa = 11.84LSS195 pKa = 3.46

Molecular weight: 21.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1333

195

852

444.3

49.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.677 ± 0.286

1.2 ± 0.415

4.126 ± 0.346

5.851 ± 1.333

3.001 ± 0.397

7.877 ± 0.658

2.401 ± 0.671

5.326 ± 1.11

3.151 ± 0.787

10.278 ± 1.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.197

2.776 ± 0.655

6.977 ± 1.179

4.051 ± 0.343

9.677 ± 0.733

7.727 ± 0.565

5.026 ± 0.044

7.052 ± 0.257

1.575 ± 0.182

3.001 ± 0.725
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski