Amino acid dipepetide frequency for Salmonella phage vB_SenS_Sasha

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.641AlaAla: 9.641 ± 1.696
1.265AlaCys: 1.265 ± 0.285
4.82AlaAsp: 4.82 ± 0.495
6.026AlaGlu: 6.026 ± 1.031
3.013AlaPhe: 3.013 ± 0.487
5.724AlaGly: 5.724 ± 0.605
1.567AlaHis: 1.567 ± 0.314
4.7AlaIle: 4.7 ± 0.538
4.459AlaLys: 4.459 ± 0.715
6.086AlaLeu: 6.086 ± 0.631
2.531AlaMet: 2.531 ± 0.416
4.64AlaAsn: 4.64 ± 0.608
3.917AlaPro: 3.917 ± 0.87
3.314AlaGln: 3.314 ± 1.099
4.82AlaArg: 4.82 ± 0.868
4.941AlaSer: 4.941 ± 0.602
5.423AlaThr: 5.423 ± 0.862
5.122AlaVal: 5.122 ± 0.685
1.205AlaTrp: 1.205 ± 0.215
2.953AlaTyr: 2.953 ± 0.381
0.0AlaXaa: 0.0 ± 0.0
Cys
1.145CysAla: 1.145 ± 0.269
0.422CysCys: 0.422 ± 0.202
1.145CysAsp: 1.145 ± 0.295
0.783CysGlu: 0.783 ± 0.201
0.422CysPhe: 0.422 ± 0.158
0.904CysGly: 0.904 ± 0.233
0.121CysHis: 0.121 ± 0.085
0.844CysIle: 0.844 ± 0.215
0.542CysLys: 0.542 ± 0.193
0.844CysLeu: 0.844 ± 0.192
0.362CysMet: 0.362 ± 0.14
0.482CysAsn: 0.482 ± 0.144
0.783CysPro: 0.783 ± 0.3
0.603CysGln: 0.603 ± 0.156
0.542CysArg: 0.542 ± 0.197
0.422CysSer: 0.422 ± 0.139
0.482CysThr: 0.482 ± 0.142
0.783CysVal: 0.783 ± 0.232
0.241CysTrp: 0.241 ± 0.142
0.723CysTyr: 0.723 ± 0.247
0.0CysXaa: 0.0 ± 0.0
Asp
4.881AspAla: 4.881 ± 0.427
0.663AspCys: 0.663 ± 0.209
4.158AspAsp: 4.158 ± 0.581
3.796AspGlu: 3.796 ± 0.369
2.832AspPhe: 2.832 ± 0.477
4.459AspGly: 4.459 ± 0.597
1.205AspHis: 1.205 ± 0.333
3.977AspIle: 3.977 ± 0.488
3.435AspLys: 3.435 ± 0.515
3.796AspLeu: 3.796 ± 0.463
2.169AspMet: 2.169 ± 0.335
2.41AspAsn: 2.41 ± 0.406
2.953AspPro: 2.953 ± 0.567
1.808AspGln: 1.808 ± 0.346
3.736AspArg: 3.736 ± 0.566
2.531AspSer: 2.531 ± 0.321
3.133AspThr: 3.133 ± 0.386
3.977AspVal: 3.977 ± 0.515
1.145AspTrp: 1.145 ± 0.255
2.953AspTyr: 2.953 ± 0.438
0.0AspXaa: 0.0 ± 0.0
Glu
5.061GluAla: 5.061 ± 0.559
0.964GluCys: 0.964 ± 0.277
2.591GluAsp: 2.591 ± 0.376
2.953GluGlu: 2.953 ± 0.431
2.29GluPhe: 2.29 ± 0.492
3.314GluGly: 3.314 ± 0.463
0.844GluHis: 0.844 ± 0.271
2.591GluIle: 2.591 ± 0.372
4.338GluLys: 4.338 ± 0.584
6.869GluLeu: 6.869 ± 0.701
1.627GluMet: 1.627 ± 0.346
3.013GluAsn: 3.013 ± 0.392
2.892GluPro: 2.892 ± 0.742
4.459GluGln: 4.459 ± 0.766
4.097GluArg: 4.097 ± 0.651
2.591GluSer: 2.591 ± 0.524
2.892GluThr: 2.892 ± 0.463
2.772GluVal: 2.772 ± 0.385
1.567GluTrp: 1.567 ± 0.407
2.591GluTyr: 2.591 ± 0.39
0.0GluXaa: 0.0 ± 0.0
Phe
2.47PheAla: 2.47 ± 0.398
0.542PheCys: 0.542 ± 0.164
1.928PheAsp: 1.928 ± 0.299
2.41PheGlu: 2.41 ± 0.396
1.205PhePhe: 1.205 ± 0.3
2.772PheGly: 2.772 ± 0.469
0.482PheHis: 0.482 ± 0.163
1.988PheIle: 1.988 ± 0.347
1.808PheLys: 1.808 ± 0.331
2.29PheLeu: 2.29 ± 0.412
0.964PheMet: 0.964 ± 0.221
1.928PheAsn: 1.928 ± 0.456
2.049PhePro: 2.049 ± 0.342
1.205PheGln: 1.205 ± 0.262
1.386PheArg: 1.386 ± 0.306
2.591PheSer: 2.591 ± 0.413
2.892PheThr: 2.892 ± 0.436
2.832PheVal: 2.832 ± 0.425
0.482PheTrp: 0.482 ± 0.176
1.205PheTyr: 1.205 ± 0.344
0.0PheXaa: 0.0 ± 0.0
Gly
5.363GlyAla: 5.363 ± 0.571
0.904GlyCys: 0.904 ± 0.257
4.881GlyAsp: 4.881 ± 0.545
4.338GlyGlu: 4.338 ± 0.468
3.013GlyPhe: 3.013 ± 0.6
5.182GlyGly: 5.182 ± 0.858
1.205GlyHis: 1.205 ± 0.263
3.977GlyIle: 3.977 ± 0.527
4.399GlyLys: 4.399 ± 0.625
5.604GlyLeu: 5.604 ± 0.601
1.687GlyMet: 1.687 ± 0.305
3.615GlyAsn: 3.615 ± 0.513
1.627GlyPro: 1.627 ± 0.254
2.35GlyGln: 2.35 ± 0.42
3.555GlyArg: 3.555 ± 0.531
4.399GlySer: 4.399 ± 0.527
4.338GlyThr: 4.338 ± 0.538
6.387GlyVal: 6.387 ± 0.681
1.446GlyTrp: 1.446 ± 0.303
3.073GlyTyr: 3.073 ± 0.405
0.0GlyXaa: 0.0 ± 0.0
His
1.145HisAla: 1.145 ± 0.269
0.362HisCys: 0.362 ± 0.13
1.265HisAsp: 1.265 ± 0.267
1.386HisGlu: 1.386 ± 0.317
0.422HisPhe: 0.422 ± 0.168
1.567HisGly: 1.567 ± 0.461
0.663HisHis: 0.663 ± 0.423
0.964HisIle: 0.964 ± 0.249
0.783HisLys: 0.783 ± 0.195
1.386HisLeu: 1.386 ± 0.351
0.181HisMet: 0.181 ± 0.109
1.085HisAsn: 1.085 ± 0.249
0.783HisPro: 0.783 ± 0.225
0.663HisGln: 0.663 ± 0.228
0.844HisArg: 0.844 ± 0.227
1.265HisSer: 1.265 ± 0.321
1.567HisThr: 1.567 ± 0.333
1.326HisVal: 1.326 ± 0.268
0.181HisTrp: 0.181 ± 0.093
0.783HisTyr: 0.783 ± 0.224
0.0HisXaa: 0.0 ± 0.0
Ile
5.302IleAla: 5.302 ± 0.652
0.844IleCys: 0.844 ± 0.256
4.579IleAsp: 4.579 ± 0.533
3.073IleGlu: 3.073 ± 0.475
1.326IlePhe: 1.326 ± 0.329
3.374IleGly: 3.374 ± 0.449
1.446IleHis: 1.446 ± 0.303
2.832IleIle: 2.832 ± 0.469
2.651IleLys: 2.651 ± 0.368
3.194IleLeu: 3.194 ± 0.398
1.265IleMet: 1.265 ± 0.276
3.495IleAsn: 3.495 ± 0.451
2.229IlePro: 2.229 ± 0.386
1.868IleGln: 1.868 ± 0.434
2.892IleArg: 2.892 ± 0.428
3.917IleSer: 3.917 ± 0.523
4.64IleThr: 4.64 ± 0.494
3.977IleVal: 3.977 ± 0.609
0.663IleTrp: 0.663 ± 0.176
2.47IleTyr: 2.47 ± 0.438
0.0IleXaa: 0.0 ± 0.0
Lys
5.001LysAla: 5.001 ± 0.653
0.964LysCys: 0.964 ± 0.311
2.29LysAsp: 2.29 ± 0.385
3.254LysGlu: 3.254 ± 0.426
1.928LysPhe: 1.928 ± 0.338
3.676LysGly: 3.676 ± 0.387
1.085LysHis: 1.085 ± 0.244
3.073LysIle: 3.073 ± 0.428
3.495LysLys: 3.495 ± 0.488
5.122LysLeu: 5.122 ± 0.732
1.446LysMet: 1.446 ± 0.309
2.29LysAsn: 2.29 ± 0.34
2.953LysPro: 2.953 ± 0.532
3.555LysGln: 3.555 ± 0.553
3.374LysArg: 3.374 ± 0.477
2.832LysSer: 2.832 ± 0.474
3.435LysThr: 3.435 ± 0.481
3.555LysVal: 3.555 ± 0.536
0.844LysTrp: 0.844 ± 0.246
2.169LysTyr: 2.169 ± 0.352
0.0LysXaa: 0.0 ± 0.0
Leu
6.688LeuAla: 6.688 ± 0.822
0.663LeuCys: 0.663 ± 0.194
4.037LeuAsp: 4.037 ± 0.429
5.122LeuGlu: 5.122 ± 0.577
2.35LeuPhe: 2.35 ± 0.379
3.495LeuGly: 3.495 ± 0.323
1.265LeuHis: 1.265 ± 0.341
4.7LeuIle: 4.7 ± 0.478
5.001LeuLys: 5.001 ± 0.606
5.604LeuLeu: 5.604 ± 0.666
2.229LeuMet: 2.229 ± 0.455
4.579LeuAsn: 4.579 ± 0.509
4.338LeuPro: 4.338 ± 0.469
3.073LeuGln: 3.073 ± 0.574
4.579LeuArg: 4.579 ± 0.636
5.363LeuSer: 5.363 ± 0.5
4.881LeuThr: 4.881 ± 0.531
4.881LeuVal: 4.881 ± 0.488
1.386LeuTrp: 1.386 ± 0.32
2.772LeuTyr: 2.772 ± 0.521
0.0LeuXaa: 0.0 ± 0.0
Met
2.591MetAla: 2.591 ± 0.331
0.241MetCys: 0.241 ± 0.126
0.964MetAsp: 0.964 ± 0.207
0.964MetGlu: 0.964 ± 0.23
1.205MetPhe: 1.205 ± 0.283
1.567MetGly: 1.567 ± 0.326
0.542MetHis: 0.542 ± 0.178
1.326MetIle: 1.326 ± 0.245
2.711MetLys: 2.711 ± 0.415
1.747MetLeu: 1.747 ± 0.33
0.723MetMet: 0.723 ± 0.251
1.567MetAsn: 1.567 ± 0.329
0.783MetPro: 0.783 ± 0.245
1.024MetGln: 1.024 ± 0.245
1.085MetArg: 1.085 ± 0.251
1.868MetSer: 1.868 ± 0.279
2.049MetThr: 2.049 ± 0.423
1.567MetVal: 1.567 ± 0.39
0.241MetTrp: 0.241 ± 0.111
0.723MetTyr: 0.723 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
4.399AsnAla: 4.399 ± 0.446
0.603AsnCys: 0.603 ± 0.204
2.953AsnAsp: 2.953 ± 0.35
2.651AsnGlu: 2.651 ± 0.429
1.446AsnPhe: 1.446 ± 0.31
5.122AsnGly: 5.122 ± 0.615
1.205AsnHis: 1.205 ± 0.281
3.495AsnIle: 3.495 ± 0.457
2.41AsnLys: 2.41 ± 0.326
3.435AsnLeu: 3.435 ± 0.665
1.446AsnMet: 1.446 ± 0.275
3.254AsnAsn: 3.254 ± 0.564
2.651AsnPro: 2.651 ± 0.424
2.711AsnGln: 2.711 ± 0.695
2.35AsnArg: 2.35 ± 0.446
3.133AsnSer: 3.133 ± 0.371
3.374AsnThr: 3.374 ± 0.573
3.495AsnVal: 3.495 ± 0.498
0.542AsnTrp: 0.542 ± 0.173
1.928AsnTyr: 1.928 ± 0.362
0.0AsnXaa: 0.0 ± 0.0
Pro
4.218ProAla: 4.218 ± 0.7
0.422ProCys: 0.422 ± 0.17
3.495ProAsp: 3.495 ± 0.39
3.615ProGlu: 3.615 ± 0.568
1.627ProPhe: 1.627 ± 0.278
3.796ProGly: 3.796 ± 0.486
0.723ProHis: 0.723 ± 0.229
1.687ProIle: 1.687 ± 0.329
1.747ProLys: 1.747 ± 0.282
2.591ProLeu: 2.591 ± 0.458
0.723ProMet: 0.723 ± 0.228
2.169ProAsn: 2.169 ± 0.452
2.772ProPro: 2.772 ± 0.74
0.844ProGln: 0.844 ± 0.184
2.29ProArg: 2.29 ± 0.372
2.169ProSer: 2.169 ± 0.38
2.711ProThr: 2.711 ± 0.5
6.086ProVal: 6.086 ± 0.978
0.904ProTrp: 0.904 ± 0.241
1.868ProTyr: 1.868 ± 0.363
0.0ProXaa: 0.0 ± 0.0
Gln
5.122GlnAla: 5.122 ± 1.216
0.603GlnCys: 0.603 ± 0.222
1.747GlnAsp: 1.747 ± 0.284
2.109GlnGlu: 2.109 ± 0.3
1.928GlnPhe: 1.928 ± 0.315
2.772GlnGly: 2.772 ± 0.378
0.723GlnHis: 0.723 ± 0.204
2.591GlnIle: 2.591 ± 0.585
2.229GlnLys: 2.229 ± 0.58
4.037GlnLeu: 4.037 ± 0.806
1.446GlnMet: 1.446 ± 0.333
2.109GlnAsn: 2.109 ± 0.432
1.808GlnPro: 1.808 ± 0.327
2.832GlnGln: 2.832 ± 0.928
2.531GlnArg: 2.531 ± 0.378
2.229GlnSer: 2.229 ± 0.709
1.928GlnThr: 1.928 ± 0.387
2.651GlnVal: 2.651 ± 0.528
0.723GlnTrp: 0.723 ± 0.194
0.844GlnTyr: 0.844 ± 0.202
0.0GlnXaa: 0.0 ± 0.0
Arg
4.579ArgAla: 4.579 ± 0.781
0.542ArgCys: 0.542 ± 0.157
3.676ArgAsp: 3.676 ± 0.518
2.892ArgGlu: 2.892 ± 0.482
2.41ArgPhe: 2.41 ± 0.414
3.073ArgGly: 3.073 ± 0.528
0.723ArgHis: 0.723 ± 0.212
3.013ArgIle: 3.013 ± 0.513
3.013ArgLys: 3.013 ± 0.601
4.82ArgLeu: 4.82 ± 0.583
1.567ArgMet: 1.567 ± 0.297
2.531ArgAsn: 2.531 ± 0.312
2.29ArgPro: 2.29 ± 0.414
2.35ArgGln: 2.35 ± 0.395
2.35ArgArg: 2.35 ± 0.467
3.133ArgSer: 3.133 ± 0.433
2.651ArgThr: 2.651 ± 0.513
3.676ArgVal: 3.676 ± 0.514
0.904ArgTrp: 0.904 ± 0.277
2.169ArgTyr: 2.169 ± 0.342
0.0ArgXaa: 0.0 ± 0.0
Ser
5.363SerAla: 5.363 ± 0.596
0.482SerCys: 0.482 ± 0.233
3.435SerAsp: 3.435 ± 0.491
2.591SerGlu: 2.591 ± 0.357
1.808SerPhe: 1.808 ± 0.312
5.363SerGly: 5.363 ± 0.672
1.265SerHis: 1.265 ± 0.299
3.796SerIle: 3.796 ± 0.521
2.651SerLys: 2.651 ± 0.42
4.158SerLeu: 4.158 ± 0.49
1.024SerMet: 1.024 ± 0.227
3.194SerAsn: 3.194 ± 0.427
2.29SerPro: 2.29 ± 0.415
3.133SerGln: 3.133 ± 0.477
2.169SerArg: 2.169 ± 0.386
3.676SerSer: 3.676 ± 0.544
3.736SerThr: 3.736 ± 0.431
5.001SerVal: 5.001 ± 0.509
0.723SerTrp: 0.723 ± 0.213
1.808SerTyr: 1.808 ± 0.331
0.0SerXaa: 0.0 ± 0.0
Thr
4.037ThrAla: 4.037 ± 0.664
0.663ThrCys: 0.663 ± 0.196
4.399ThrAsp: 4.399 ± 0.507
3.796ThrGlu: 3.796 ± 0.365
1.687ThrPhe: 1.687 ± 0.374
6.568ThrGly: 6.568 ± 0.699
0.723ThrHis: 0.723 ± 0.227
3.194ThrIle: 3.194 ± 0.445
2.651ThrLys: 2.651 ± 0.335
5.785ThrLeu: 5.785 ± 0.634
0.964ThrMet: 0.964 ± 0.316
2.953ThrAsn: 2.953 ± 0.454
4.097ThrPro: 4.097 ± 0.509
2.169ThrGln: 2.169 ± 0.415
3.254ThrArg: 3.254 ± 0.432
3.194ThrSer: 3.194 ± 0.425
4.218ThrThr: 4.218 ± 0.619
5.182ThrVal: 5.182 ± 0.73
0.723ThrTrp: 0.723 ± 0.204
2.109ThrTyr: 2.109 ± 0.339
0.0ThrXaa: 0.0 ± 0.0
Val
6.086ValAla: 6.086 ± 0.812
0.482ValCys: 0.482 ± 0.146
4.459ValAsp: 4.459 ± 0.485
4.941ValGlu: 4.941 ± 0.589
2.772ValPhe: 2.772 ± 0.391
4.76ValGly: 4.76 ± 0.589
1.326ValHis: 1.326 ± 0.321
4.037ValIle: 4.037 ± 0.607
4.519ValLys: 4.519 ± 0.484
6.327ValLeu: 6.327 ± 0.594
1.928ValMet: 1.928 ± 0.28
4.097ValAsn: 4.097 ± 0.516
2.651ValPro: 2.651 ± 0.463
3.073ValGln: 3.073 ± 0.432
2.953ValArg: 2.953 ± 0.48
3.856ValSer: 3.856 ± 0.508
5.242ValThr: 5.242 ± 0.817
4.519ValVal: 4.519 ± 0.427
0.783ValTrp: 0.783 ± 0.184
3.073ValTyr: 3.073 ± 0.46
0.0ValXaa: 0.0 ± 0.0
Trp
0.783TrpAla: 0.783 ± 0.262
0.181TrpCys: 0.181 ± 0.118
0.964TrpAsp: 0.964 ± 0.212
1.265TrpGlu: 1.265 ± 0.242
0.663TrpPhe: 0.663 ± 0.244
1.085TrpGly: 1.085 ± 0.221
0.723TrpHis: 0.723 ± 0.288
0.723TrpIle: 0.723 ± 0.225
1.265TrpLys: 1.265 ± 0.335
1.386TrpLeu: 1.386 ± 0.275
0.241TrpMet: 0.241 ± 0.095
1.145TrpAsn: 1.145 ± 0.296
0.482TrpPro: 0.482 ± 0.183
0.301TrpGln: 0.301 ± 0.142
0.844TrpArg: 0.844 ± 0.338
1.205TrpSer: 1.205 ± 0.33
0.603TrpThr: 0.603 ± 0.194
1.024TrpVal: 1.024 ± 0.262
0.482TrpTrp: 0.482 ± 0.178
0.723TrpTyr: 0.723 ± 0.293
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.29TyrAla: 2.29 ± 0.408
0.844TyrCys: 0.844 ± 0.255
2.229TyrAsp: 2.229 ± 0.374
2.35TyrGlu: 2.35 ± 0.422
1.085TyrPhe: 1.085 ± 0.292
2.772TyrGly: 2.772 ± 0.406
0.783TyrHis: 0.783 ± 0.288
2.531TyrIle: 2.531 ± 0.419
2.229TyrLys: 2.229 ± 0.453
1.928TyrLeu: 1.928 ± 0.335
0.904TyrMet: 0.904 ± 0.189
2.049TyrAsn: 2.049 ± 0.358
2.109TyrPro: 2.109 ± 0.418
1.627TyrGln: 1.627 ± 0.373
2.711TyrArg: 2.711 ± 0.451
2.35TyrSer: 2.35 ± 0.366
2.169TyrThr: 2.169 ± 0.378
3.073TyrVal: 3.073 ± 0.433
0.904TyrTrp: 0.904 ± 0.194
1.326TyrTyr: 1.326 ± 0.28
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 81 proteins (16597 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski