Mamestra configurata nucleopolyhedrovirus A
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 168 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8QLD9|Q8QLD9_9ABAC Maco-A 95 OS=Mamestra configurata nucleopolyhedrovirus A OX=207830 PE=4 SV=1
MM1 pKa = 7.44 NSAHH5 pKa = 7.51 DD6 pKa = 4.27 DD7 pKa = 3.25 VSMQANTVFNISYY20 pKa = 10.05 EE21 pKa = 4.24 EE22 pKa = 3.89 QDD24 pKa = 4.29 EE25 pKa = 4.26 EE26 pKa = 6.3 DD27 pKa = 5.27 EE28 pKa = 4.91 DD29 pKa = 4.4 DD30 pKa = 3.84 HH31 pKa = 9.76 DD32 pKa = 4.43 NFIFNVTEE40 pKa = 4.05 YY41 pKa = 10.87 INFLRR46 pKa = 11.84 LLSGQLTLEE55 pKa = 4.18 DD56 pKa = 3.48 VNYY59 pKa = 9.94 EE60 pKa = 3.96 FTMDD64 pKa = 4.99 SGDD67 pKa = 3.35 EE68 pKa = 4.12 DD69 pKa = 3.97 FDD71 pKa = 4.67 YY72 pKa = 11.63 DD73 pKa = 5.35 HH74 pKa = 7.24 GLLDD78 pKa = 3.27 IRR80 pKa = 11.84 QYY82 pKa = 9.87 ITVTTAVDD90 pKa = 3.8 TFQPRR95 pKa = 11.84 NFIMRR100 pKa = 11.84 ADD102 pKa = 4.4 CSRR105 pKa = 11.84 TDD107 pKa = 3.32 TPIINDD113 pKa = 3.93 TCCICLDD120 pKa = 4.04 ALLEE124 pKa = 4.08 TDD126 pKa = 3.81 KK127 pKa = 11.5 QIAQLDD133 pKa = 3.93 ACQHH137 pKa = 5.7 GFCAEE142 pKa = 4.17 CLFMWLKK149 pKa = 6.85 THH151 pKa = 6.48 CVCPVCRR158 pKa = 11.84 TLCTKK163 pKa = 10.42 INLIKK168 pKa = 10.8
Molecular weight: 19.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.868
IPC2_protein 3.999
IPC_protein 3.986
Toseland 3.783
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.923
Rodwell 3.821
Grimsley 3.681
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.342
Thurlkill 3.834
EMBOSS 3.935
Sillero 4.113
Patrickios 0.502
IPC_peptide 3.973
IPC2_peptide 4.088
IPC2.peptide.svr19 3.986
Protein with the highest isoelectric point:
>tr|Q8QLE9|Q8QLE9_9ABAC BV/ODV-C42 OS=Mamestra configurata nucleopolyhedrovirus A OX=207830 GN=bv/odv-c42 PE=4 SV=1
MM1 pKa = 7.44 VYY3 pKa = 10.01 RR4 pKa = 11.84 RR5 pKa = 11.84 SSSSGRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 SSNGRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 SSSGRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 SSNGRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 SSGGYY41 pKa = 8.18 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 SSGYY48 pKa = 7.94 HH49 pKa = 5.4 RR50 pKa = 11.84 RR51 pKa = 11.84 PGRR54 pKa = 11.84 PRR56 pKa = 11.84 GSRR59 pKa = 11.84 TKK61 pKa = 10.54 RR62 pKa = 11.84 RR63 pKa = 11.84 GSSNPYY69 pKa = 8.74 GYY71 pKa = 8.53 TRR73 pKa = 11.84 RR74 pKa = 11.84 HH75 pKa = 4.79 YY76 pKa = 11.07
Molecular weight: 9.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 11.155
IPC_protein 12.34
Toseland 12.471
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.954
Rodwell 12.003
Grimsley 12.515
Solomon 12.983
Lehninger 12.881
Nozaki 12.471
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.711
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.102
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
168
0
168
47173
50
1212
280.8
32.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.575 ± 0.145
2.468 ± 0.129
6.43 ± 0.148
5.363 ± 0.177
4.598 ± 0.128
3.506 ± 0.144
2.493 ± 0.085
6.68 ± 0.126
6.605 ± 0.222
9.06 ± 0.149
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.749 ± 0.093
7.299 ± 0.145
3.572 ± 0.142
3.841 ± 0.156
5.005 ± 0.138
6.099 ± 0.138
5.957 ± 0.151
7.099 ± 0.146
0.774 ± 0.055
4.827 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here