Rhodococcus phage Finch

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 228 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1JXP2|A0A2P1JXP2_9CAUD ParB-like nuclease domain protein OS=Rhodococcus phage Finch OX=2094144 GN=210 PE=4 SV=1
MM1 pKa = 7.62WLLAPRR7 pKa = 11.84PVGRR11 pKa = 11.84SVTEE15 pKa = 3.94PEE17 pKa = 4.29EE18 pKa = 5.12DD19 pKa = 3.18LDD21 pKa = 4.24APKK24 pKa = 10.47VRR26 pKa = 11.84YY27 pKa = 9.62NSAPYY32 pKa = 10.68LDD34 pKa = 3.94ISYY37 pKa = 10.68DD38 pKa = 3.6CSGWNCGSNTIEE50 pKa = 4.11WEE52 pKa = 4.22DD53 pKa = 4.35GEE55 pKa = 4.89WMCSDD60 pKa = 5.36CKK62 pKa = 9.09TTWSDD67 pKa = 3.25PDD69 pKa = 3.39NGQLYY74 pKa = 9.69SEE76 pKa = 4.73WSGEE80 pKa = 4.15PYY82 pKa = 10.51DD83 pKa = 4.48GPLVDD88 pKa = 4.14TYY90 pKa = 11.35GDD92 pKa = 3.62PVEE95 pKa = 4.38RR96 pKa = 11.84TT97 pKa = 3.44

Molecular weight: 11.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1JXS7|A0A2P1JXS7_9CAUD Uncharacterized protein OS=Rhodococcus phage Finch OX=2094144 GN=177 PE=4 SV=1
MM1 pKa = 7.15SHH3 pKa = 6.85SGPPKK8 pKa = 9.24TIMVVPDD15 pKa = 2.99SRR17 pKa = 11.84GRR19 pKa = 11.84VVLTGVRR26 pKa = 11.84QLAHH30 pKa = 7.0HH31 pKa = 5.56YY32 pKa = 10.26NIRR35 pKa = 11.84QEE37 pKa = 3.95PDD39 pKa = 2.77GTVILTPVRR48 pKa = 11.84WW49 pKa = 3.41

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

228

0

228

44420

26

1149

194.8

21.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.275 ± 0.235

0.995 ± 0.087

6.988 ± 0.182

7.078 ± 0.26

3.276 ± 0.104

7.643 ± 0.184

2.105 ± 0.128

4.214 ± 0.116

4.408 ± 0.132

7.663 ± 0.171

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.097

3.647 ± 0.131

5.104 ± 0.174

3.744 ± 0.117

5.95 ± 0.171

6.207 ± 0.157

6.529 ± 0.206

7.364 ± 0.192

1.785 ± 0.087

3.33 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski