Thiobacillus denitrificans (strain ATCC 25259)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Thiobacillaceae; Thiobacillus; Thiobacillus denitrificans

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2826 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3SI52|Q3SI52_THIDA Nucleotide binding protein OS=Thiobacillus denitrificans (strain ATCC 25259) OX=292415 GN=Tbd_1728 PE=3 SV=1
MM1 pKa = 7.26NRR3 pKa = 11.84NKK5 pKa = 10.2ILLATLLAASVFAGPSAYY23 pKa = 9.79AQSWVGTADD32 pKa = 3.4YY33 pKa = 8.75TAGSSEE39 pKa = 3.72GDD41 pKa = 3.4EE42 pKa = 4.68DD43 pKa = 4.63VVGPFNTYY51 pKa = 10.15DD52 pKa = 3.11AGAGVVLLQPTGMSGGTFTFDD73 pKa = 2.97GVYY76 pKa = 9.88QSYY79 pKa = 7.72VTKK82 pKa = 10.6HH83 pKa = 5.56EE84 pKa = 4.21LSGVPVVAAGLDD96 pKa = 3.5DD97 pKa = 4.31TYY99 pKa = 11.63EE100 pKa = 4.06LTAVATFTEE109 pKa = 4.9SVSAIGGITVTGGTFNLYY127 pKa = 10.3RR128 pKa = 11.84DD129 pKa = 3.7TTRR132 pKa = 11.84DD133 pKa = 2.83RR134 pKa = 11.84DD135 pKa = 3.69YY136 pKa = 8.93TTDD139 pKa = 3.09SGFSDD144 pKa = 4.4GEE146 pKa = 4.42AILTGTITSGAGAAAADD163 pKa = 4.04LSFGVTDD170 pKa = 3.18ITIAITDD177 pKa = 3.63YY178 pKa = 9.69DD179 pKa = 3.86TSVFNPDD186 pKa = 3.64TIATGGGIFTLRR198 pKa = 11.84MGSEE202 pKa = 3.94LDD204 pKa = 3.5APFLSGITSVGGNTYY219 pKa = 10.72DD220 pKa = 4.11AGSGDD225 pKa = 4.59LLYY228 pKa = 10.97AADD231 pKa = 5.25GYY233 pKa = 11.22LALAVPEE240 pKa = 4.37AEE242 pKa = 4.56TYY244 pKa = 11.9AMMLAGLGLVGFMARR259 pKa = 11.84RR260 pKa = 11.84RR261 pKa = 11.84TRR263 pKa = 11.84SMII266 pKa = 3.64

Molecular weight: 27.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q3SER3|Q3SER3_THIDA Uroporphyrinogen decarboxylase OS=Thiobacillus denitrificans (strain ATCC 25259) OX=292415 GN=hemE PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.29QPSTVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.38RR14 pKa = 11.84THH16 pKa = 5.85GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.62TKK25 pKa = 10.49AGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.07GRR39 pKa = 11.84ARR41 pKa = 11.84LAVV44 pKa = 3.42

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2826

0

2826

894101

30

3472

316.4

34.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.01 ± 0.056

0.893 ± 0.016

5.588 ± 0.032

5.737 ± 0.041

3.561 ± 0.027

8.109 ± 0.05

2.327 ± 0.025

4.358 ± 0.031

3.314 ± 0.04

10.972 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.025

2.539 ± 0.026

5.132 ± 0.036

3.419 ± 0.029

7.427 ± 0.048

4.964 ± 0.031

4.936 ± 0.032

7.631 ± 0.038

1.351 ± 0.02

2.43 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski