Bacillus virus Finn
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M1IEY5|M1IEY5_9CAUD Uncharacterized protein OS=Bacillus virus Finn OX=1273742 GN=FINN_48 PE=4 SV=1
MM1 pKa = 7.33 NEE3 pKa = 3.5 NRR5 pKa = 11.84 FPHH8 pKa = 5.7 LTQYY12 pKa = 8.38 EE13 pKa = 4.54 HH14 pKa = 6.84 PHH16 pKa = 6.15 SEE18 pKa = 4.47 LYY20 pKa = 10.02 TSQEE24 pKa = 4.27 VNDD27 pKa = 5.02 GDD29 pKa = 4.28 PDD31 pKa = 4.09 CPSVLDD37 pKa = 3.97 YY38 pKa = 11.26 QVDD41 pKa = 4.01 TNDD44 pKa = 3.98 KK45 pKa = 10.86 DD46 pKa = 3.11 ITLAINDD53 pKa = 4.04 GDD55 pKa = 4.57 DD56 pKa = 5.1 DD57 pKa = 4.42 YY58 pKa = 12.32 ACFILNKK65 pKa = 10.14 DD66 pKa = 4.17 HH67 pKa = 7.77 IPFLEE72 pKa = 4.0 QLLSDD77 pKa = 4.31 LKK79 pKa = 11.13 EE80 pKa = 4.08 VTKK83 pKa = 11.1 DD84 pKa = 3.24 EE85 pKa = 4.33
Molecular weight: 9.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.935
IPC_protein 3.91
Toseland 3.694
ProMoST 4.05
Dawson 3.91
Bjellqvist 4.113
Wikipedia 3.859
Rodwell 3.745
Grimsley 3.605
Solomon 3.897
Lehninger 3.859
Nozaki 4.037
DTASelect 4.279
Thurlkill 3.757
EMBOSS 3.872
Sillero 4.037
Patrickios 1.926
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.945
Protein with the highest isoelectric point:
>tr|M1IEW1|M1IEW1_9CAUD Tape measure protein OS=Bacillus virus Finn OX=1273742 GN=FINN_23 PE=4 SV=1
MM1 pKa = 7.48 SNVNMMISVDD11 pKa = 3.95 LNNVPEE17 pKa = 4.24 NLARR21 pKa = 11.84 AARR24 pKa = 11.84 QVSDD28 pKa = 3.56 FKK30 pKa = 11.28 IPLRR34 pKa = 11.84 QSAVYY39 pKa = 9.16 MEE41 pKa = 4.55 GSISRR46 pKa = 11.84 RR47 pKa = 11.84 FSSGGGSQGKK57 pKa = 7.77 WKK59 pKa = 10.04 RR60 pKa = 11.84 LSPATIRR67 pKa = 11.84 RR68 pKa = 11.84 HH69 pKa = 4.26 PHH71 pKa = 5.88 RR72 pKa = 11.84 SGGTPLSDD80 pKa = 3.1 TGRR83 pKa = 11.84 LKK85 pKa = 10.92 QSVTSGAVKK94 pKa = 10.14 QYY96 pKa = 10.82 SPKK99 pKa = 9.68 QLRR102 pKa = 11.84 YY103 pKa = 7.94 TLGANVKK110 pKa = 8.54 YY111 pKa = 10.9 AKK113 pKa = 9.79 VHH115 pKa = 5.39 NFGGGRR121 pKa = 11.84 IPARR125 pKa = 11.84 PFMYY129 pKa = 10.04 TDD131 pKa = 2.97 AKK133 pKa = 10.86 DD134 pKa = 3.36 RR135 pKa = 11.84 QEE137 pKa = 3.84 VVKK140 pKa = 10.76 IFTEE144 pKa = 4.43 FLRR147 pKa = 11.84 GVANQQ152 pKa = 3.21
Molecular weight: 16.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.295
IPC2_protein 10.058
IPC_protein 11.038
Toseland 11.14
ProMoST 11.155
Dawson 11.199
Bjellqvist 10.994
Wikipedia 11.491
Rodwell 11.345
Grimsley 11.242
Solomon 11.447
Lehninger 11.389
Nozaki 11.125
DTASelect 10.994
Thurlkill 11.14
EMBOSS 11.579
Sillero 11.155
Patrickios 11.067
IPC_peptide 11.462
IPC2_peptide 10.116
IPC2.peptide.svr19 8.419
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
76
0
76
14779
29
1026
194.5
22.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.137 ± 0.357
0.92 ± 0.133
6.225 ± 0.282
8.032 ± 0.495
4.547 ± 0.201
6.739 ± 0.376
2.158 ± 0.182
6.672 ± 0.212
8.038 ± 0.523
7.93 ± 0.369
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.855 ± 0.216
5.068 ± 0.358
3.309 ± 0.193
3.613 ± 0.176
4.371 ± 0.248
6.232 ± 0.395
6.022 ± 0.431
6.178 ± 0.244
1.137 ± 0.118
3.816 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here