Candidatus Microthrix parvicella RN1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Acidimicrobiia; Acidimicrobiales; Microthrixaceae; Candidatus Microthrix; Candidatus Microthrix parvicella

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4245 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4Z4S7|R4Z4S7_9ACTN Electron-transferring-flavoprotein dehydrogenase OS=Candidatus Microthrix parvicella RN1 OX=1229780 GN=BN381_250065 PE=3 SV=1
MM1 pKa = 7.79AKK3 pKa = 10.06PAGDD7 pKa = 3.77AMATLPSWTEE17 pKa = 3.74PGLVWAPSVLQTDD30 pKa = 3.42DD31 pKa = 4.03DD32 pKa = 4.92GFVLSYY38 pKa = 10.75TSLDD42 pKa = 3.55SRR44 pKa = 11.84SGLQCVGAARR54 pKa = 11.84ASSPLGPFVDD64 pKa = 5.04GSDD67 pKa = 4.55DD68 pKa = 3.67PFVCQRR74 pKa = 11.84EE75 pKa = 4.11LGGTIDD81 pKa = 4.21ASPFVDD87 pKa = 5.59DD88 pKa = 6.18DD89 pKa = 3.83GTPYY93 pKa = 11.41LLFKK97 pKa = 11.09NDD99 pKa = 4.16GNCCDD104 pKa = 3.75ITTQLWTQQLSADD117 pKa = 3.48GMTLVGDD124 pKa = 5.1AIALLEE130 pKa = 4.23TTEE133 pKa = 4.19DD134 pKa = 3.3WEE136 pKa = 5.02GPLIEE141 pKa = 5.03GPSMTTLGDD150 pKa = 4.19SYY152 pKa = 10.93WLLYY156 pKa = 9.78SAYY159 pKa = 9.63DD160 pKa = 3.67WNSADD165 pKa = 3.57YY166 pKa = 11.02SVGLAQCEE174 pKa = 4.65SPVGPCTKK182 pKa = 9.92RR183 pKa = 11.84DD184 pKa = 4.01GPWLANHH191 pKa = 7.02GDD193 pKa = 3.4ASGPGGAEE201 pKa = 4.34DD202 pKa = 4.1FTDD205 pKa = 3.66ADD207 pKa = 3.45GRR209 pKa = 11.84RR210 pKa = 11.84WMLYY214 pKa = 9.37HH215 pKa = 7.2AWVTDD220 pKa = 3.86KK221 pKa = 10.83IGYY224 pKa = 5.82EE225 pKa = 4.1TGGEE229 pKa = 3.74RR230 pKa = 11.84SLFAVPLDD238 pKa = 3.84LRR240 pKa = 11.84SGEE243 pKa = 4.04PVARR247 pKa = 11.84GLSEE251 pKa = 4.88DD252 pKa = 3.86SADD255 pKa = 3.41

Molecular weight: 27.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4YY95|R4YY95_9ACTN Uncharacterized protein OS=Candidatus Microthrix parvicella RN1 OX=1229780 GN=BN381_20122 PE=4 SV=1
MM1 pKa = 7.57GSLIKK6 pKa = 10.25KK7 pKa = 8.32RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.27RR11 pKa = 11.84MRR13 pKa = 11.84KK14 pKa = 9.05KK15 pKa = 9.86KK16 pKa = 9.7HH17 pKa = 5.75KK18 pKa = 10.01KK19 pKa = 8.87LLRR22 pKa = 11.84KK23 pKa = 7.68TRR25 pKa = 11.84HH26 pKa = 3.96QRR28 pKa = 11.84RR29 pKa = 11.84SKK31 pKa = 10.57KK32 pKa = 10.38

Molecular weight: 4.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4245

0

4245

1298969

14

2399

306.0

32.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.559 ± 0.047

0.835 ± 0.013

6.511 ± 0.032

5.589 ± 0.033

2.875 ± 0.024

9.398 ± 0.04

2.132 ± 0.019

3.77 ± 0.024

1.988 ± 0.024

9.894 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.04 ± 0.017

2.262 ± 0.02

5.963 ± 0.034

2.803 ± 0.019

7.333 ± 0.046

5.904 ± 0.028

6.099 ± 0.031

8.715 ± 0.034

1.608 ± 0.019

1.721 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski