Lactobacillus aviarius subsp. araffinosus DSM 20653
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1386 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R1ZC37|A0A0R1ZC37_9LACO Aminotransferase OS=Lactobacillus aviarius subsp. araffinosus DSM 20653 OX=1423820 GN=FC64_GL000665 PE=3 SV=1
MM1 pKa = 7.56 EE2 pKa = 6.26 KK3 pKa = 10.72 DD4 pKa = 3.76 DD5 pKa = 5.78 FINSMLTYY13 pKa = 10.6 LHH15 pKa = 7.18 LDD17 pKa = 3.98 DD18 pKa = 6.43 DD19 pKa = 4.87 PEE21 pKa = 4.41 TMQEE25 pKa = 3.58 LTAIVDD31 pKa = 3.71 GSIATIINGINQSLTYY47 pKa = 10.83 DD48 pKa = 3.9 DD49 pKa = 5.73 LKK51 pKa = 11.36 ADD53 pKa = 3.77 NQFIMALRR61 pKa = 11.84 TLVTQTYY68 pKa = 9.67 YY69 pKa = 11.04 DD70 pKa = 3.59 RR71 pKa = 11.84 EE72 pKa = 4.18 LANGYY77 pKa = 10.15 SFGFLSYY84 pKa = 10.43 VAPLQAKK91 pKa = 9.23 YY92 pKa = 11.03 SEE94 pKa = 4.35 VGNDD98 pKa = 4.39 DD99 pKa = 3.67 EE100 pKa = 4.82 TDD102 pKa = 3.26 SS103 pKa = 4.08
Molecular weight: 11.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.719
IPC_protein 3.694
Toseland 3.478
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.389
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.075
Thurlkill 3.541
EMBOSS 3.668
Sillero 3.821
Patrickios 0.846
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.74
Protein with the highest isoelectric point:
>tr|A0A0R1ZI74|A0A0R1ZI74_9LACO Uncharacterized protein OS=Lactobacillus aviarius subsp. araffinosus DSM 20653 OX=1423820 GN=FC64_GL000363 PE=4 SV=1
MM1 pKa = 7.4 RR2 pKa = 11.84 QIGLNALLTIISNFTFILITFKK24 pKa = 10.6 ILRR27 pKa = 11.84 DD28 pKa = 3.71 LRR30 pKa = 11.84 LDD32 pKa = 3.47 YY33 pKa = 10.98 YY34 pKa = 10.93 VNKK37 pKa = 9.73 EE38 pKa = 3.74 RR39 pKa = 11.84 QRR41 pKa = 11.84 TFRR44 pKa = 11.84 LFLILVSTALGFLVSQFFLNFIDD67 pKa = 3.8 SVRR70 pKa = 11.84 NLGYY74 pKa = 10.98 LLL76 pKa = 4.33
Molecular weight: 9.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.911
IPC_protein 10.818
Toseland 10.54
ProMoST 10.394
Dawson 10.716
Bjellqvist 10.496
Wikipedia 10.979
Rodwell 10.789
Grimsley 10.804
Solomon 10.833
Lehninger 10.789
Nozaki 10.496
DTASelect 10.496
Thurlkill 10.584
EMBOSS 10.95
Sillero 10.643
Patrickios 10.599
IPC_peptide 10.833
IPC2_peptide 9.487
IPC2.peptide.svr19 8.257
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1386
0
1386
429889
50
1515
310.2
34.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.179 ± 0.068
0.659 ± 0.018
5.763 ± 0.063
5.896 ± 0.082
4.453 ± 0.056
6.519 ± 0.057
2.225 ± 0.032
7.788 ± 0.072
7.294 ± 0.055
9.446 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.862 ± 0.032
5.344 ± 0.065
3.452 ± 0.029
4.723 ± 0.074
3.885 ± 0.053
5.567 ± 0.063
5.504 ± 0.047
6.809 ± 0.049
0.983 ± 0.025
3.65 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here