Mycobacterium sp. 1423905.2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; unclassified Mycobacterium

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4711 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A3I7Y1|A0A1A3I7Y1_9MYCO DNA-binding protein OS=Mycobacterium sp. 1423905.2 OX=1856859 GN=A9W95_01225 PE=4 SV=1
MM1 pKa = 7.55SYY3 pKa = 10.62VIAMPEE9 pKa = 4.08LMQDD13 pKa = 3.38AADD16 pKa = 3.64NLAGIRR22 pKa = 11.84SALAAVAADD31 pKa = 3.34IAGPTTGIAAAAQDD45 pKa = 4.16EE46 pKa = 4.85VSVALASLFGNFGNEE61 pKa = 3.83FQVLNAQAQAFHH73 pKa = 6.81DD74 pKa = 3.64QFVNLMRR81 pKa = 11.84AGTGAYY87 pKa = 10.31LSTEE91 pKa = 4.16TANVEE96 pKa = 3.93QALLNGPAADD106 pKa = 3.5MVAPVVAEE114 pKa = 4.04AQALSQFGTTVVAPYY129 pKa = 9.74QALYY133 pKa = 11.01ADD135 pKa = 4.31TAANLQSLEE144 pKa = 4.12NAVLQDD150 pKa = 3.48PAPFLRR156 pKa = 11.84QLVANQVGYY165 pKa = 10.02GQAIAGAIQTLPANVANLPAALEE188 pKa = 4.11ALVPADD194 pKa = 3.7PAGVLQGIVNQQIGYY209 pKa = 8.26AQTVATALGSAAQDD223 pKa = 3.49FGAGVAGLPAGFQAAVGDD241 pKa = 4.31LVAGNAPAAVRR252 pKa = 11.84DD253 pKa = 3.56IATSYY258 pKa = 11.81GNLFLTGLTANQDD271 pKa = 3.14MTTFLINITPAGTLGDD287 pKa = 4.8LLPILAIPGQMAQNFTNLLPVGSVPGVISQHH318 pKa = 4.85FTNLLLTLSDD328 pKa = 3.71TSQTLDD334 pKa = 3.54LNTGILHH341 pKa = 6.02VGLPLVLGLDD351 pKa = 4.38AIGPAATAVNALGSSATAFVNAVQIGDD378 pKa = 3.95GLGAAAALLDD388 pKa = 4.14APAVVANGFLNGHH401 pKa = 5.89ATLPLSVALGEE412 pKa = 4.41LTTTTNIPLGGILTPGQFASLTLEE436 pKa = 4.1VFGMSGTLPLFGTPFGGILPALLTFLPEE464 pKa = 3.98QLAQAIGAPVPVV476 pKa = 3.92

Molecular weight: 47.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A3HZP3|A0A1A3HZP3_9MYCO Uncharacterized protein OS=Mycobacterium sp. 1423905.2 OX=1856859 GN=A9W95_18935 PE=4 SV=1
MM1 pKa = 7.09QFPRR5 pKa = 11.84RR6 pKa = 11.84SQARR10 pKa = 11.84RR11 pKa = 11.84SRR13 pKa = 11.84AQRR16 pKa = 11.84VAAVQFPRR24 pKa = 11.84RR25 pKa = 11.84SQARR29 pKa = 11.84RR30 pKa = 11.84SRR32 pKa = 11.84AQRR35 pKa = 11.84VAAVQFPRR43 pKa = 11.84RR44 pKa = 11.84SQARR48 pKa = 11.84RR49 pKa = 11.84SRR51 pKa = 11.84AQRR54 pKa = 11.84VAAVQFPRR62 pKa = 11.84RR63 pKa = 11.84SQARR67 pKa = 11.84RR68 pKa = 11.84SRR70 pKa = 11.84AQRR73 pKa = 11.84VAAVQFPRR81 pKa = 11.84RR82 pKa = 11.84SQARR86 pKa = 11.84RR87 pKa = 11.84SRR89 pKa = 11.84AQRR92 pKa = 11.84VAAVQFF98 pKa = 3.94

Molecular weight: 11.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4711

0

4711

1535493

29

4139

325.9

34.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.106 ± 0.066

0.818 ± 0.011

6.077 ± 0.036

5.042 ± 0.037

3.041 ± 0.024

9.17 ± 0.157

2.263 ± 0.015

4.152 ± 0.025

2.16 ± 0.025

9.81 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.999 ± 0.018

2.435 ± 0.038

5.896 ± 0.039

3.258 ± 0.02

7.17 ± 0.039

5.448 ± 0.022

5.93 ± 0.025

8.548 ± 0.043

1.506 ± 0.015

2.171 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski