Chimpanzee polyomavirus Bob
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E5AX30|E5AX30_9POLY VP1 protein OS=Chimpanzee polyomavirus Bob OX=2035845 GN=VP1 PE=3 SV=1
MM1 pKa = 7.01 TVEE4 pKa = 4.18 GLSGIEE10 pKa = 4.47 ALAQLGWTAEE20 pKa = 4.19 QFSNMAFISTTFSNAIGYY38 pKa = 8.57 GVLFQTVSGISSLVSAGIRR57 pKa = 11.84 LGTSVSSVNRR67 pKa = 11.84 HH68 pKa = 3.58 QTEE71 pKa = 4.1 QEE73 pKa = 3.93 LEE75 pKa = 4.16 TLFGKK80 pKa = 9.98 IAHH83 pKa = 6.47 FLHH86 pKa = 6.58 VNLAFHH92 pKa = 7.44 LDD94 pKa = 3.46 PFDD97 pKa = 3.35 WCGSIGTTMPPEE109 pKa = 4.47 FSNLTLDD116 pKa = 3.85 QLSKK120 pKa = 10.81 LALIIEE126 pKa = 4.27 NGRR129 pKa = 11.84 WVIQRR134 pKa = 11.84 SPTHH138 pKa = 7.03 DD139 pKa = 3.82 PLFEE143 pKa = 6.25 SGDD146 pKa = 3.89 IIDD149 pKa = 4.1 MFGPPGGARR158 pKa = 11.84 QRR160 pKa = 11.84 VTPDD164 pKa = 2.19 WMLPLILRR172 pKa = 11.84 LNGASQEE179 pKa = 4.07 KK180 pKa = 10.73 SSLCVNSNQSS190 pKa = 2.84
Molecular weight: 20.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.025
IPC2_protein 5.041
IPC_protein 4.94
Toseland 4.927
ProMoST 5.092
Dawson 4.952
Bjellqvist 5.067
Wikipedia 4.825
Rodwell 4.876
Grimsley 4.863
Solomon 4.952
Lehninger 4.914
Nozaki 5.08
DTASelect 5.219
Thurlkill 4.94
EMBOSS 4.889
Sillero 5.156
Patrickios 4.101
IPC_peptide 4.965
IPC2_peptide 5.156
IPC2.peptide.svr19 5.098
Protein with the highest isoelectric point:
>tr|E5AX32|E5AX32_9POLY Small T antigen OS=Chimpanzee polyomavirus Bob OX=2035845 GN=small t PE=4 SV=1
MM1 pKa = 7.97 DD2 pKa = 4.63 KK3 pKa = 10.86 VLEE6 pKa = 4.33 KK7 pKa = 10.41 SDD9 pKa = 4.2 RR10 pKa = 11.84 EE11 pKa = 4.03 MLIEE15 pKa = 3.95 LLGIPSYY22 pKa = 11.62 AFGNFPIMKK31 pKa = 8.51 TAYY34 pKa = 9.07 KK35 pKa = 9.52 RR36 pKa = 11.84 ASKK39 pKa = 9.97 IYY41 pKa = 10.0 HH42 pKa = 6.58 PDD44 pKa = 3.14 KK45 pKa = 11.07 GGSSEE50 pKa = 5.93 KK51 pKa = 10.35 MMLLNSLWQKK61 pKa = 9.64 FQEE64 pKa = 4.29 GLVDD68 pKa = 3.31 IRR70 pKa = 11.84 GSEE73 pKa = 4.16 VCQVSFSDD81 pKa = 4.43 CYY83 pKa = 10.86 DD84 pKa = 3.17 VKK86 pKa = 11.01 LLRR89 pKa = 11.84 NCGTVKK95 pKa = 10.27 HH96 pKa = 5.46 FHH98 pKa = 6.72 EE99 pKa = 4.89 IFLRR103 pKa = 11.84 SPQCLQKK110 pKa = 11.06 GSAVCNCITSTLFNQHH126 pKa = 5.47 RR127 pKa = 11.84 QIKK130 pKa = 8.61 LLCNKK135 pKa = 9.81 RR136 pKa = 11.84 CLTWGEE142 pKa = 4.54 CFCFSCFLIWFGMDD156 pKa = 3.83 FKK158 pKa = 11.02 WEE160 pKa = 4.09 SFDD163 pKa = 2.97 MWKK166 pKa = 10.58 YY167 pKa = 10.84 VIAEE171 pKa = 4.31 MPTGLLQLPPSKK183 pKa = 10.97 YY184 pKa = 9.86 KK185 pKa = 10.6 FSSFPLVLKK194 pKa = 10.77
Molecular weight: 22.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.691
IPC2_protein 7.863
IPC_protein 7.673
Toseland 7.19
ProMoST 8.375
Dawson 8.404
Bjellqvist 8.843
Wikipedia 8.302
Rodwell 8.434
Grimsley 7.161
Solomon 8.478
Lehninger 8.507
Nozaki 9.165
DTASelect 8.507
Thurlkill 8.58
EMBOSS 8.609
Sillero 8.931
Patrickios 4.151
IPC_peptide 8.478
IPC2_peptide 7.878
IPC2.peptide.svr19 7.99
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1805
190
615
361.0
40.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.427 ± 0.515
2.382 ± 0.439
4.598 ± 0.502
6.371 ± 0.502
5.097 ± 0.809
6.593 ± 0.852
2.05 ± 0.117
5.762 ± 0.242
6.482 ± 1.273
10.693 ± 0.892
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.992 ± 0.154
4.155 ± 0.437
5.817 ± 1.374
4.321 ± 0.258
4.1 ± 0.369
7.756 ± 1.141
5.485 ± 0.807
5.263 ± 0.313
1.33 ± 0.274
2.327 ± 0.548
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here