Tomato leaf curl Bangalore virus-[Ban5] satellite DNA beta
Average proteome isoelectric point is 5.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A9QXD0|A9QXD0_9VIRU C1 protein OS=Tomato leaf curl Bangalore virus-[Ban5] satellite DNA beta OX=486898 GN=C1 PE=4 SV=1
MM1 pKa = 7.12 TIKK4 pKa = 10.47 YY5 pKa = 9.83 VNRR8 pKa = 11.84 NGLMFIINVRR18 pKa = 11.84 LKK20 pKa = 10.45 GDD22 pKa = 3.62 DD23 pKa = 3.77 SIKK26 pKa = 10.64 VHH28 pKa = 6.13 IQLTSTRR35 pKa = 11.84 SPALVKK41 pKa = 10.41 RR42 pKa = 11.84 KK43 pKa = 9.11 FVIPYY48 pKa = 8.31 GHH50 pKa = 6.84 NGLIPPFDD58 pKa = 4.19 FNNLEE63 pKa = 3.87 EE64 pKa = 4.9 GIRR67 pKa = 11.84 NILTIMYY74 pKa = 8.84 RR75 pKa = 11.84 DD76 pKa = 3.37 TMFDD80 pKa = 3.54 EE81 pKa = 5.77 FKK83 pKa = 10.96 HH84 pKa = 6.32 EE85 pKa = 5.41 DD86 pKa = 3.45 MTEE89 pKa = 4.35 LIDD92 pKa = 3.73 MLIMTEE98 pKa = 4.09 APVVISGFDD107 pKa = 3.14 DD108 pKa = 3.43 MMFVLCVCVV117 pKa = 3.4
Molecular weight: 13.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.373
IPC2_protein 5.41
IPC_protein 5.321
Toseland 5.499
ProMoST 5.563
Dawson 5.448
Bjellqvist 5.512
Wikipedia 5.397
Rodwell 5.385
Grimsley 5.537
Solomon 5.448
Lehninger 5.423
Nozaki 5.639
DTASelect 5.83
Thurlkill 5.639
EMBOSS 5.626
Sillero 5.715
Patrickios 4.202
IPC_peptide 5.448
IPC2_peptide 5.715
IPC2.peptide.svr19 5.763
Protein with the highest isoelectric point:
>tr|A9QXD0|A9QXD0_9VIRU C1 protein OS=Tomato leaf curl Bangalore virus-[Ban5] satellite DNA beta OX=486898 GN=C1 PE=4 SV=1
MM1 pKa = 7.12 TIKK4 pKa = 10.47 YY5 pKa = 9.83 VNRR8 pKa = 11.84 NGLMFIINVRR18 pKa = 11.84 LKK20 pKa = 10.45 GDD22 pKa = 3.62 DD23 pKa = 3.77 SIKK26 pKa = 10.64 VHH28 pKa = 6.13 IQLTSTRR35 pKa = 11.84 SPALVKK41 pKa = 10.41 RR42 pKa = 11.84 KK43 pKa = 9.11 FVIPYY48 pKa = 8.31 GHH50 pKa = 6.84 NGLIPPFDD58 pKa = 4.19 FNNLEE63 pKa = 3.87 EE64 pKa = 4.9 GIRR67 pKa = 11.84 NILTIMYY74 pKa = 8.84 RR75 pKa = 11.84 DD76 pKa = 3.37 TMFDD80 pKa = 3.54 EE81 pKa = 5.77 FKK83 pKa = 10.96 HH84 pKa = 6.32 EE85 pKa = 5.41 DD86 pKa = 3.45 MTEE89 pKa = 4.35 LIDD92 pKa = 3.73 MLIMTEE98 pKa = 4.09 APVVISGFDD107 pKa = 3.14 DD108 pKa = 3.43 MMFVLCVCVV117 pKa = 3.4
Molecular weight: 13.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.373
IPC2_protein 5.41
IPC_protein 5.321
Toseland 5.499
ProMoST 5.563
Dawson 5.448
Bjellqvist 5.512
Wikipedia 5.397
Rodwell 5.385
Grimsley 5.537
Solomon 5.448
Lehninger 5.423
Nozaki 5.639
DTASelect 5.83
Thurlkill 5.639
EMBOSS 5.626
Sillero 5.715
Patrickios 4.202
IPC_peptide 5.448
IPC2_peptide 5.715
IPC2.peptide.svr19 5.763
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
117
117
117
117.0
13.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.709 ± 0.0
1.709 ± 0.0
7.692 ± 0.0
5.128 ± 0.0
6.838 ± 0.0
5.128 ± 0.0
2.564 ± 0.0
11.111 ± 0.0
5.128 ± 0.0
8.547 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
7.692 ± 0.0
5.983 ± 0.0
4.274 ± 0.0
0.855 ± 0.0
5.128 ± 0.0
3.419 ± 0.0
5.983 ± 0.0
8.547 ± 0.0
0.0 ± 0.0
2.564 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here