Mycobacterium phage Nerujay

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6WER1|A0A0F6WER1_9CAUD RecB-like protein OS=Mycobacterium phage Nerujay OX=1647308 GN=74 PE=4 SV=1
MM1 pKa = 7.35PQTVHH6 pKa = 6.12VLPVDD11 pKa = 3.73DD12 pKa = 5.86LIEE15 pKa = 6.01HH16 pKa = 7.01EE17 pKa = 5.59DD18 pKa = 3.6VGDD21 pKa = 4.07DD22 pKa = 3.95CVCGPEE28 pKa = 4.04IEE30 pKa = 4.64PVFDD34 pKa = 5.12ADD36 pKa = 4.79GACGWVITHH45 pKa = 6.86HH46 pKa = 6.49SLDD49 pKa = 3.22GRR51 pKa = 11.84EE52 pKa = 3.86RR53 pKa = 11.84FEE55 pKa = 3.97

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6WEI5|A0A0F6WEI5_9CAUD Uncharacterized protein OS=Mycobacterium phage Nerujay OX=1647308 GN=90 PE=4 SV=1
MM1 pKa = 7.23GWEE4 pKa = 3.86SSDD7 pKa = 3.24RR8 pKa = 11.84RR9 pKa = 11.84EE10 pKa = 4.33RR11 pKa = 11.84LPADD15 pKa = 3.28WPRR18 pKa = 11.84IRR20 pKa = 11.84RR21 pKa = 11.84EE22 pKa = 3.77VLRR25 pKa = 11.84AAGHH29 pKa = 6.4RR30 pKa = 11.84CQIRR34 pKa = 11.84YY35 pKa = 9.91ADD37 pKa = 3.88ICTGMATEE45 pKa = 4.27VDD47 pKa = 3.34HH48 pKa = 7.08VRR50 pKa = 11.84YY51 pKa = 9.51RR52 pKa = 11.84DD53 pKa = 3.7EE54 pKa = 4.41EE55 pKa = 4.64SPTQASCKK63 pKa = 8.6PCHH66 pKa = 5.93ARR68 pKa = 11.84KK69 pKa = 9.58SAMEE73 pKa = 4.09GVAQRR78 pKa = 11.84AKK80 pKa = 10.29LRR82 pKa = 11.84AMKK85 pKa = 9.94KK86 pKa = 9.63RR87 pKa = 11.84PPPRR91 pKa = 11.84HH92 pKa = 5.89PGRR95 pKa = 11.84RR96 pKa = 11.84TPRR99 pKa = 11.84EE100 pKa = 3.98DD101 pKa = 3.06QQ102 pKa = 3.4

Molecular weight: 11.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

16725

28

826

172.4

18.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.895 ± 0.393

0.712 ± 0.122

6.703 ± 0.193

6.535 ± 0.201

3.091 ± 0.179

8.885 ± 0.533

1.895 ± 0.154

4.855 ± 0.195

4.138 ± 0.286

8.419 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.117 ± 0.127

3.229 ± 0.167

5.477 ± 0.201

3.235 ± 0.177

6.35 ± 0.361

6.158 ± 0.258

6.194 ± 0.272

7.253 ± 0.241

2.069 ± 0.127

2.792 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski