Amino acid dipepetide frequency for Trifolium pratense (Red clover)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.349AlaAla: 5.349 ± 0.024	1.139AlaCys: 1.139 ± 0.01	2.936AlaAsp: 2.936 ± 0.015	3.904AlaGlu: 3.904 ± 0.018	2.589AlaPhe: 2.589 ± 0.012	3.562AlaGly: 3.562 ± 0.018	1.228AlaHis: 1.228 ± 0.009	3.754AlaIle: 3.754 ± 0.018	3.823AlaLys: 3.823 ± 0.014	5.976AlaLeu: 5.976 ± 0.024	1.734AlaMet: 1.734 ± 0.011	2.7AlaAsn: 2.7 ± 0.012	2.539AlaPro: 2.539 ± 0.016	1.989AlaGln: 1.989 ± 0.012	3.049AlaArg: 3.049 ± 0.015	5.358AlaSer: 5.358 ± 0.023	3.485AlaThr: 3.485 ± 0.017	4.355AlaVal: 4.355 ± 0.018	0.777AlaTrp: 0.777 ± 0.007	1.733AlaTyr: 1.733 ± 0.012	0.017AlaXaa: 0.017 ± 0.001
Cys	0.927CysAla: 0.927 ± 0.01	0.492CysCys: 0.492 ± 0.007	0.989CysAsp: 0.989 ± 0.008	0.917CysGlu: 0.917 ± 0.008	0.91CysPhe: 0.91 ± 0.007	1.365CysGly: 1.365 ± 0.012	0.498CysHis: 0.498 ± 0.006	1.022CysIle: 1.022 ± 0.008	1.171CysLys: 1.171 ± 0.01	1.803CysLeu: 1.803 ± 0.012	0.426CysMet: 0.426 ± 0.005	0.942CysAsn: 0.942 ± 0.009	0.936CysPro: 0.936 ± 0.009	0.604CysGln: 0.604 ± 0.007	0.948CysArg: 0.948 ± 0.008	1.719CysSer: 1.719 ± 0.012	0.832CysThr: 0.832 ± 0.009	1.149CysVal: 1.149 ± 0.01	0.282CysTrp: 0.282 ± 0.004	0.577CysTyr: 0.577 ± 0.006	0.005CysXaa: 0.005 ± 0.001
Asp	3.358AspAla: 3.358 ± 0.017	1.006AspCys: 1.006 ± 0.008	4.165AspAsp: 4.165 ± 0.024	4.274AspGlu: 4.274 ± 0.019	2.455AspPhe: 2.455 ± 0.014	3.764AspGly: 3.764 ± 0.015	1.293AspHis: 1.293 ± 0.01	3.364AspIle: 3.364 ± 0.016	2.96AspLys: 2.96 ± 0.015	5.061AspLeu: 5.061 ± 0.02	1.357AspMet: 1.357 ± 0.009	2.477AspAsn: 2.477 ± 0.013	2.459AspPro: 2.459 ± 0.013	1.781AspGln: 1.781 ± 0.012	2.317AspArg: 2.317 ± 0.014	4.386AspSer: 4.386 ± 0.02	2.385AspThr: 2.385 ± 0.014	3.859AspVal: 3.859 ± 0.017	0.763AspTrp: 0.763 ± 0.007	1.615AspTyr: 1.615 ± 0.011	0.015AspXaa: 0.015 ± 0.001
Glu	4.482GluAla: 4.482 ± 0.019	0.954GluCys: 0.954 ± 0.008	4.074GluAsp: 4.074 ± 0.021	6.354GluGlu: 6.354 ± 0.033	2.487GluPhe: 2.487 ± 0.011	3.663GluGly: 3.663 ± 0.016	1.283GluHis: 1.283 ± 0.01	3.941GluIle: 3.941 ± 0.016	4.882GluLys: 4.882 ± 0.022	5.894GluLeu: 5.894 ± 0.025	1.775GluMet: 1.775 ± 0.013	3.433GluAsn: 3.433 ± 0.018	2.04GluPro: 2.04 ± 0.013	2.18GluGln: 2.18 ± 0.011	3.187GluArg: 3.187 ± 0.018	4.587GluSer: 4.587 ± 0.021	3.152GluThr: 3.152 ± 0.014	4.332GluVal: 4.332 ± 0.021	0.767GluTrp: 0.767 ± 0.008	1.677GluTyr: 1.677 ± 0.011	0.018GluXaa: 0.018 ± 0.001
Phe	2.389PheAla: 2.389 ± 0.014	0.895PheCys: 0.895 ± 0.008	2.528PheAsp: 2.528 ± 0.014	2.516PheGlu: 2.516 ± 0.013	1.818PhePhe: 1.818 ± 0.012	3.007PheGly: 3.007 ± 0.016	1.189PheHis: 1.189 ± 0.009	2.262PheIle: 2.262 ± 0.014	2.421PheLys: 2.421 ± 0.014	4.094PheLeu: 4.094 ± 0.018	0.979PheMet: 0.979 ± 0.009	2.06PheAsn: 2.06 ± 0.012	2.033PhePro: 2.033 ± 0.012	1.572PheGln: 1.572 ± 0.01	1.922PheArg: 1.922 ± 0.011	3.854PheSer: 3.854 ± 0.017	2.108PheThr: 2.108 ± 0.012	2.827PheVal: 2.827 ± 0.015	0.582PheTrp: 0.582 ± 0.006	1.25PheTyr: 1.25 ± 0.011	0.011PheXaa: 0.011 ± 0.001
Gly	3.461GlyAla: 3.461 ± 0.02	1.315GlyCys: 1.315 ± 0.01	3.33GlyAsp: 3.33 ± 0.016	3.592GlyGlu: 3.592 ± 0.018	3.072GlyPhe: 3.072 ± 0.014	5.321GlyGly: 5.321 ± 0.045	1.479GlyHis: 1.479 ± 0.012	3.679GlyIle: 3.679 ± 0.016	4.129GlyLys: 4.129 ± 0.02	5.596GlyLeu: 5.596 ± 0.019	1.495GlyMet: 1.495 ± 0.011	3.184GlyAsn: 3.184 ± 0.018	2.144GlyPro: 2.144 ± 0.013	1.893GlyGln: 1.893 ± 0.013	3.383GlyArg: 3.383 ± 0.018	5.482GlySer: 5.482 ± 0.024	3.153GlyThr: 3.153 ± 0.018	4.233GlyVal: 4.233 ± 0.023	0.938GlyTrp: 0.938 ± 0.009	2.166GlyTyr: 2.166 ± 0.014	0.019GlyXaa: 0.019 ± 0.001
His	1.379HisAla: 1.379 ± 0.009	0.531HisCys: 0.531 ± 0.006	1.254HisAsp: 1.254 ± 0.011	1.346HisGlu: 1.346 ± 0.01	1.15HisPhe: 1.15 ± 0.01	1.693HisGly: 1.693 ± 0.011	0.969HisHis: 0.969 ± 0.013	1.335HisIle: 1.335 ± 0.01	1.284HisLys: 1.284 ± 0.01	2.409HisLeu: 2.409 ± 0.015	0.577HisMet: 0.577 ± 0.006	1.188HisAsn: 1.188 ± 0.008	1.301HisPro: 1.301 ± 0.01	1.046HisGln: 1.046 ± 0.01	1.292HisArg: 1.292 ± 0.01	1.997HisSer: 1.997 ± 0.013	1.055HisThr: 1.055 ± 0.008	1.677HisVal: 1.677 ± 0.011	0.312HisTrp: 0.312 ± 0.004	0.72HisTyr: 0.72 ± 0.007	0.008HisXaa: 0.008 ± 0.001
Ile	3.72IleAla: 3.72 ± 0.017	1.11IleCys: 1.11 ± 0.009	3.264IleAsp: 3.264 ± 0.015	3.513IleGlu: 3.513 ± 0.017	2.335IlePhe: 2.335 ± 0.012	3.638IleGly: 3.638 ± 0.018	1.444IleHis: 1.444 ± 0.009	3.097IleIle: 3.097 ± 0.014	3.289IleLys: 3.289 ± 0.015	5.351IleLeu: 5.351 ± 0.019	1.223IleMet: 1.223 ± 0.009	2.557IleAsn: 2.557 ± 0.013	3.159IlePro: 3.159 ± 0.018	2.127IleGln: 2.127 ± 0.011	2.589IleArg: 2.589 ± 0.013	4.946IleSer: 4.946 ± 0.019	2.828IleThr: 2.828 ± 0.015	3.741IleVal: 3.741 ± 0.017	0.817IleTrp: 0.817 ± 0.008	1.502IleTyr: 1.502 ± 0.012	0.016IleXaa: 0.016 ± 0.001
Lys	3.912LysAla: 3.912 ± 0.018	1.072LysCys: 1.072 ± 0.008	3.529LysAsp: 3.529 ± 0.017	4.918LysGlu: 4.918 ± 0.022	2.394LysPhe: 2.394 ± 0.012	3.845LysGly: 3.845 ± 0.018	1.466LysHis: 1.466 ± 0.011	3.603LysIle: 3.603 ± 0.018	5.177LysLys: 5.177 ± 0.031	6.341LysLeu: 6.341 ± 0.023	1.634LysMet: 1.634 ± 0.011	3.116LysAsn: 3.116 ± 0.016	2.771LysPro: 2.771 ± 0.017	2.404LysGln: 2.404 ± 0.013	3.471LysArg: 3.471 ± 0.02	4.908LysSer: 4.908 ± 0.021	3.113LysThr: 3.113 ± 0.014	4.232LysVal: 4.232 ± 0.019	0.927LysTrp: 0.927 ± 0.009	1.727LysTyr: 1.727 ± 0.011	0.017LysXaa: 0.017 ± 0.001
Leu	5.917LeuAla: 5.917 ± 0.022	1.823LeuCys: 1.823 ± 0.013	5.094LeuAsp: 5.094 ± 0.016	6.159LeuGlu: 6.159 ± 0.027	3.774LeuPhe: 3.774 ± 0.018	5.47LeuGly: 5.47 ± 0.019	2.626LeuHis: 2.626 ± 0.015	4.811LeuIle: 4.811 ± 0.019	6.602LeuLys: 6.602 ± 0.023	9.245LeuLeu: 9.245 ± 0.031	2.172LeuMet: 2.172 ± 0.012	4.352LeuAsn: 4.352 ± 0.017	4.91LeuPro: 4.91 ± 0.02	4.18LeuGln: 4.18 ± 0.02	4.998LeuArg: 4.998 ± 0.018	8.289LeuSer: 8.289 ± 0.033	4.568LeuThr: 4.568 ± 0.02	6.263LeuVal: 6.263 ± 0.023	1.238LeuTrp: 1.238 ± 0.01	2.444LeuTyr: 2.444 ± 0.015	0.027LeuXaa: 0.027 ± 0.001
Met	1.862MetAla: 1.862 ± 0.011	0.371MetCys: 0.371 ± 0.005	1.42MetAsp: 1.42 ± 0.011	1.934MetGlu: 1.934 ± 0.012	0.882MetPhe: 0.882 ± 0.007	1.434MetGly: 1.434 ± 0.011	0.601MetHis: 0.601 ± 0.007	1.3MetIle: 1.3 ± 0.01	1.839MetLys: 1.839 ± 0.009	2.251MetLeu: 2.251 ± 0.012	0.741MetMet: 0.741 ± 0.008	1.182MetAsn: 1.182 ± 0.01	1.017MetPro: 1.017 ± 0.01	0.984MetGln: 0.984 ± 0.009	1.169MetArg: 1.169 ± 0.009	1.835MetSer: 1.835 ± 0.011	1.138MetThr: 1.138 ± 0.009	1.7MetVal: 1.7 ± 0.012	0.293MetTrp: 0.293 ± 0.004	0.593MetTyr: 0.593 ± 0.006	0.007MetXaa: 0.007 ± 0.001
Asn	2.748AsnAla: 2.748 ± 0.015	0.961AsnCys: 0.961 ± 0.01	2.532AsnAsp: 2.532 ± 0.014	2.907AsnGlu: 2.907 ± 0.015	2.144AsnPhe: 2.144 ± 0.012	3.461AsnGly: 3.461 ± 0.017	1.311AsnHis: 1.311 ± 0.01	2.892AsnIle: 2.892 ± 0.015	2.868AsnLys: 2.868 ± 0.016	4.966AsnLeu: 4.966 ± 0.023	1.236AsnMet: 1.236 ± 0.009	3.169AsnAsn: 3.169 ± 0.023	2.39AsnPro: 2.39 ± 0.016	1.911AsnGln: 1.911 ± 0.012	2.086AsnArg: 2.086 ± 0.012	4.241AsnSer: 4.241 ± 0.02	2.293AsnThr: 2.293 ± 0.014	3.29AsnVal: 3.29 ± 0.016	0.668AsnTrp: 0.668 ± 0.006	1.412AsnTyr: 1.412 ± 0.01	0.015AsnXaa: 0.015 ± 0.001
Pro	2.5ProAla: 2.5 ± 0.015	0.693ProCys: 0.693 ± 0.007	2.388ProAsp: 2.388 ± 0.015	2.837ProGlu: 2.837 ± 0.014	1.989ProPhe: 1.989 ± 0.014	2.321ProGly: 2.321 ± 0.015	1.127ProHis: 1.127 ± 0.01	2.559ProIle: 2.559 ± 0.014	2.818ProLys: 2.818 ± 0.017	4.143ProLeu: 4.143 ± 0.017	0.976ProMet: 0.976 ± 0.009	2.529ProAsn: 2.529 ± 0.015	3.781ProPro: 3.781 ± 0.046	1.808ProGln: 1.808 ± 0.014	2.153ProArg: 2.153 ± 0.013	4.866ProSer: 4.866 ± 0.023	2.73ProThr: 2.73 ± 0.015	2.901ProVal: 2.901 ± 0.02	0.565ProTrp: 0.565 ± 0.006	1.351ProTyr: 1.351 ± 0.012	0.014ProXaa: 0.014 ± 0.001
Gln	2.193GlnAla: 2.193 ± 0.014	0.595GlnCys: 0.595 ± 0.006	1.675GlnAsp: 1.675 ± 0.011	2.368GlnGlu: 2.368 ± 0.011	1.495GlnPhe: 1.495 ± 0.01	2.095GlnGly: 2.095 ± 0.013	1.035GlnHis: 1.035 ± 0.009	2.071GlnIle: 2.071 ± 0.012	2.336GlnLys: 2.336 ± 0.014	3.527GlnLeu: 3.527 ± 0.016	0.999GlnMet: 0.999 ± 0.008	2.015GlnAsn: 2.015 ± 0.013	1.763GlnPro: 1.763 ± 0.013	2.21GlnGln: 2.21 ± 0.023	1.978GlnArg: 1.978 ± 0.012	2.796GlnSer: 2.796 ± 0.016	1.785GlnThr: 1.785 ± 0.012	2.381GlnVal: 2.381 ± 0.014	0.498GlnTrp: 0.498 ± 0.006	0.945GlnTyr: 0.945 ± 0.008	0.011GlnXaa: 0.011 ± 0.001
Arg	2.8ArgAla: 2.8 ± 0.015	0.911ArgCys: 0.911 ± 0.009	2.573ArgAsp: 2.573 ± 0.014	3.058ArgGlu: 3.058 ± 0.013	2.147ArgPhe: 2.147 ± 0.011	3.06ArgGly: 3.06 ± 0.017	1.198ArgHis: 1.198 ± 0.009	2.793ArgIle: 2.793 ± 0.013	3.675ArgLys: 3.675 ± 0.018	4.715ArgLeu: 4.715 ± 0.017	1.269ArgMet: 1.269 ± 0.008	2.52ArgAsn: 2.52 ± 0.013	2.076ArgPro: 2.076 ± 0.012	1.747ArgGln: 1.747 ± 0.012	3.454ArgArg: 3.454 ± 0.02	3.958ArgSer: 3.958 ± 0.021	2.322ArgThr: 2.322 ± 0.012	3.183ArgVal: 3.183 ± 0.014	0.731ArgTrp: 0.731 ± 0.007	1.438ArgTyr: 1.438 ± 0.01	0.014ArgXaa: 0.014 ± 0.001
Ser	4.69SerAla: 4.69 ± 0.02	1.609SerCys: 1.609 ± 0.012	4.571SerAsp: 4.571 ± 0.016	4.729SerGlu: 4.729 ± 0.021	3.849SerPhe: 3.849 ± 0.019	5.361SerGly: 5.361 ± 0.02	2.066SerHis: 2.066 ± 0.011	4.814SerIle: 4.814 ± 0.02	5.197SerLys: 5.197 ± 0.022	8.423SerLeu: 8.423 ± 0.031	2.027SerMet: 2.027 ± 0.011	4.461SerAsn: 4.461 ± 0.019	4.237SerPro: 4.237 ± 0.026	2.98SerGln: 2.98 ± 0.016	4.02SerArg: 4.02 ± 0.02	10.395SerSer: 10.395 ± 0.043	4.681SerThr: 4.681 ± 0.021	5.22SerVal: 5.22 ± 0.019	1.227SerTrp: 1.227 ± 0.009	2.306SerTyr: 2.306 ± 0.012	0.025SerXaa: 0.025 ± 0.001
Thr	3.149ThrAla: 3.149 ± 0.017	0.908ThrCys: 0.908 ± 0.008	2.407ThrAsp: 2.407 ± 0.012	2.766ThrGlu: 2.766 ± 0.015	2.137ThrPhe: 2.137 ± 0.013	3.04ThrGly: 3.04 ± 0.014	1.12ThrHis: 1.12 ± 0.008	3.083ThrIle: 3.083 ± 0.014	3.076ThrLys: 3.076 ± 0.016	4.82ThrLeu: 4.82 ± 0.019	1.254ThrMet: 1.254 ± 0.01	2.434ThrAsn: 2.434 ± 0.013	2.637ThrPro: 2.637 ± 0.017	1.65ThrGln: 1.65 ± 0.011	2.314ThrArg: 2.314 ± 0.012	4.666ThrSer: 4.666 ± 0.02	3.416ThrThr: 3.416 ± 0.017	3.383ThrVal: 3.383 ± 0.017	0.724ThrTrp: 0.724 ± 0.008	1.4ThrTyr: 1.4 ± 0.012	0.016ThrXaa: 0.016 ± 0.001
Val	4.687ValAla: 4.687 ± 0.02	1.19ValCys: 1.19 ± 0.009	4.032ValAsp: 4.032 ± 0.016	4.646ValGlu: 4.646 ± 0.019	2.78ValPhe: 2.78 ± 0.014	4.051ValGly: 4.051 ± 0.021	1.489ValHis: 1.489 ± 0.01	3.595ValIle: 3.595 ± 0.017	4.228ValLys: 4.228 ± 0.018	6.225ValLeu: 6.225 ± 0.019	1.607ValMet: 1.607 ± 0.011	2.915ValAsn: 2.915 ± 0.014	3.109ValPro: 3.109 ± 0.016	2.273ValGln: 2.273 ± 0.015	3.004ValArg: 3.004 ± 0.014	5.353ValSer: 5.353 ± 0.018	3.407ValThr: 3.407 ± 0.015	5.203ValVal: 5.203 ± 0.023	0.828ValTrp: 0.828 ± 0.009	1.965ValTyr: 1.965 ± 0.012	0.019ValXaa: 0.019 ± 0.001
Trp	0.68TrpAla: 0.68 ± 0.007	0.3TrpCys: 0.3 ± 0.005	0.715TrpAsp: 0.715 ± 0.007	0.802TrpGlu: 0.802 ± 0.008	0.583TrpPhe: 0.583 ± 0.006	0.792TrpGly: 0.792 ± 0.008	0.339TrpHis: 0.339 ± 0.004	0.833TrpIle: 0.833 ± 0.007	1.107TrpLys: 1.107 ± 0.009	1.325TrpLeu: 1.325 ± 0.01	0.34TrpMet: 0.34 ± 0.005	0.827TrpAsn: 0.827 ± 0.008	0.479TrpPro: 0.479 ± 0.006	0.459TrpGln: 0.459 ± 0.006	0.906TrpArg: 0.906 ± 0.008	1.021TrpSer: 1.021 ± 0.009	0.637TrpThr: 0.637 ± 0.007	0.82TrpVal: 0.82 ± 0.008	0.271TrpTrp: 0.271 ± 0.005	0.369TrpTyr: 0.369 ± 0.006	0.003TrpXaa: 0.003 ± 0.0
Tyr	1.778TyrAla: 1.778 ± 0.014	0.634TyrCys: 0.634 ± 0.008	1.624TyrAsp: 1.624 ± 0.011	1.601TyrGlu: 1.601 ± 0.011	1.315TyrPhe: 1.315 ± 0.01	2.102TyrGly: 2.102 ± 0.014	0.755TyrHis: 0.755 ± 0.008	1.44TyrIle: 1.44 ± 0.011	1.674TyrLys: 1.674 ± 0.013	2.77TyrLeu: 2.77 ± 0.016	0.725TyrMet: 0.725 ± 0.006	1.424TyrAsn: 1.424 ± 0.011	1.256TyrPro: 1.256 ± 0.01	0.965TyrGln: 0.965 ± 0.008	1.388TyrArg: 1.388 ± 0.011	2.208TyrSer: 2.208 ± 0.013	1.298TyrThr: 1.298 ± 0.01	1.839TyrVal: 1.839 ± 0.011	0.394TyrTrp: 0.394 ± 0.005	0.984TyrTyr: 0.984 ± 0.008	0.008TyrXaa: 0.008 ± 0.001
Xaa	0.016XaaAla: 0.016 ± 0.001	0.008XaaCys: 0.008 ± 0.001	0.015XaaAsp: 0.015 ± 0.001	0.019XaaGlu: 0.019 ± 0.001	0.012XaaPhe: 0.012 ± 0.001	0.018XaaGly: 0.018 ± 0.001	0.007XaaHis: 0.007 ± 0.001	0.016XaaIle: 0.016 ± 0.001	0.02XaaLys: 0.02 ± 0.001	0.027XaaLeu: 0.027 ± 0.001	0.008XaaMet: 0.008 ± 0.001	0.013XaaAsn: 0.013 ± 0.001	0.011XaaPro: 0.011 ± 0.001	0.009XaaGln: 0.009 ± 0.001	0.015XaaArg: 0.015 ± 0.001	0.021XaaSer: 0.021 ± 0.001	0.014XaaThr: 0.014 ± 0.001	0.02XaaVal: 0.02 ± 0.001	0.005XaaTrp: 0.005 ± 0.001	0.009XaaTyr: 0.009 ± 0.001	0.029XaaXaa: 0.029 ± 0.002

Statistics based on 60146 proteins (15246222 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---