Lactobacillus xujianguonis
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2074 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A437SWM1|A0A437SWM1_9LACO Nucleoside hydrolase OS=Lactobacillus xujianguonis OX=2495899 GN=EJK17_02530 PE=4 SV=1
MM1 pKa = 7.33 KK2 pKa = 10.49 AKK4 pKa = 9.88 IVYY7 pKa = 10.65 ASMTGNDD14 pKa = 2.97 EE15 pKa = 5.17 DD16 pKa = 3.78 MAEE19 pKa = 4.1 MLEE22 pKa = 5.07 EE23 pKa = 4.68 DD24 pKa = 3.52 LQDD27 pKa = 3.39 YY28 pKa = 10.2 GFEE31 pKa = 4.16 VDD33 pKa = 4.16 SSDD36 pKa = 4.9 VSFTDD41 pKa = 2.99 ATDD44 pKa = 3.59 YY45 pKa = 11.62 LDD47 pKa = 5.27 ADD49 pKa = 4.23 LCIMITYY56 pKa = 8.87 TYY58 pKa = 11.05 GEE60 pKa = 4.1 GKK62 pKa = 7.95 MTDD65 pKa = 3.32 EE66 pKa = 4.35 TADD69 pKa = 4.32 FYY71 pKa = 11.94 AQLKK75 pKa = 9.98 EE76 pKa = 3.71 LDD78 pKa = 3.85 LNGKK82 pKa = 8.7 YY83 pKa = 9.69 FAVMGSGDD91 pKa = 3.44 KK92 pKa = 10.61 TYY94 pKa = 11.34 GEE96 pKa = 4.52 HH97 pKa = 5.99 FCEE100 pKa = 4.57 NVFDD104 pKa = 4.23 FEE106 pKa = 5.34 KK107 pKa = 10.96 AFKK110 pKa = 10.46 QCGATEE116 pKa = 4.17 IVPAVTIEE124 pKa = 3.85 NAPDD128 pKa = 4.87 DD129 pKa = 5.21 DD130 pKa = 7.7 DD131 pKa = 5.64 IDD133 pKa = 4.59 QIDD136 pKa = 3.71 TAAEE140 pKa = 3.91 EE141 pKa = 4.48 MNDD144 pKa = 3.76 KK145 pKa = 11.18 LNAA148 pKa = 4.04
Molecular weight: 16.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.617
IPC_protein 3.63
Toseland 3.414
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.567
Rodwell 3.452
Grimsley 3.325
Solomon 3.617
Lehninger 3.567
Nozaki 3.745
DTASelect 3.973
Thurlkill 3.465
EMBOSS 3.579
Sillero 3.757
Patrickios 1.024
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.704
Protein with the highest isoelectric point:
>tr|A0A437SSY2|A0A437SSY2_9LACO Heavy-metal-associated domain-containing protein OS=Lactobacillus xujianguonis OX=2495899 GN=EJK17_10145 PE=4 SV=1
MM1 pKa = 7.7 PKK3 pKa = 9.34 MRR5 pKa = 11.84 THH7 pKa = 6.88 RR8 pKa = 11.84 ASAKK12 pKa = 9.49 RR13 pKa = 11.84 FKK15 pKa = 10.03 RR16 pKa = 11.84 TANGGLKK23 pKa = 9.5 RR24 pKa = 11.84 HH25 pKa = 6.15 HH26 pKa = 7.23 AFTGHH31 pKa = 6.77 RR32 pKa = 11.84 FHH34 pKa = 7.54 GKK36 pKa = 6.24 TKK38 pKa = 9.47 KK39 pKa = 9.37 QRR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.45 LRR45 pKa = 11.84 KK46 pKa = 9.11 AAMVSSSDD54 pKa = 3.45 MKK56 pKa = 10.55 RR57 pKa = 11.84 IKK59 pKa = 10.8 QMVSQMRR66 pKa = 3.38
Molecular weight: 7.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.429
IPC2_protein 10.935
IPC_protein 12.501
Toseland 12.676
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.471
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.193
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.05
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2074
0
2074
616652
31
2155
297.3
33.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.593 ± 0.058
0.558 ± 0.013
6.027 ± 0.059
5.675 ± 0.055
4.307 ± 0.044
6.423 ± 0.056
2.197 ± 0.027
7.119 ± 0.057
7.96 ± 0.061
9.412 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.616 ± 0.028
5.294 ± 0.049
3.609 ± 0.03
4.251 ± 0.036
3.815 ± 0.04
5.7 ± 0.046
5.698 ± 0.042
6.789 ± 0.043
1.105 ± 0.021
3.851 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here