Streptococcus phage Javan393
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6AAS4|A0A4D6AAS4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan393 OX=2548146 GN=Javan393_0030 PE=4 SV=1
MM1 pKa = 7.35 GVYY4 pKa = 10.4 SSLDD8 pKa = 3.14 KK9 pKa = 11.39 ARR11 pKa = 11.84 DD12 pKa = 3.68 YY13 pKa = 11.28 IDD15 pKa = 3.06 WAGYY19 pKa = 9.63 RR20 pKa = 11.84 YY21 pKa = 10.17 EE22 pKa = 5.58 DD23 pKa = 4.41 GFFQEE28 pKa = 4.17 YY29 pKa = 10.4 SEE31 pKa = 4.14 YY32 pKa = 11.11 LEE34 pKa = 4.64 TYY36 pKa = 10.42 SKK38 pKa = 11.48 EE39 pKa = 3.99 EE40 pKa = 4.18 DD41 pKa = 3.39 NGVHH45 pKa = 5.17 KK46 pKa = 9.5 TISILEE52 pKa = 4.14 RR53 pKa = 11.84 EE54 pKa = 4.11 LDD56 pKa = 3.45 KK57 pKa = 11.55 DD58 pKa = 3.35 LGEE61 pKa = 4.51 LVFEE65 pKa = 4.57 AVGTEE70 pKa = 3.84
Molecular weight: 8.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.106
IPC2_protein 4.215
IPC_protein 4.101
Toseland 3.935
ProMoST 4.177
Dawson 4.05
Bjellqvist 4.266
Wikipedia 3.935
Rodwell 3.935
Grimsley 3.846
Solomon 4.05
Lehninger 3.999
Nozaki 4.177
DTASelect 4.304
Thurlkill 3.961
EMBOSS 3.948
Sillero 4.215
Patrickios 2.943
IPC_peptide 4.05
IPC2_peptide 4.202
IPC2.peptide.svr19 4.137
Protein with the highest isoelectric point:
>tr|A0A4D6A9Q4|A0A4D6A9Q4_9CAUD Antirepressor protein OS=Streptococcus phage Javan393 OX=2548146 GN=Javan393_0055 PE=4 SV=1
MM1 pKa = 7.68 KK2 pKa = 10.56 FILKK6 pKa = 10.0 NIHH9 pKa = 6.18 TIILLIGIGLVMYY22 pKa = 9.57 GFFLISQMVGYY33 pKa = 9.91 IGSGVLAIMLATYY46 pKa = 10.16 IDD48 pKa = 3.58 RR49 pKa = 11.84 SVRR52 pKa = 3.37
Molecular weight: 5.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.132
IPC2_protein 9.706
IPC_protein 9.94
Toseland 9.75
ProMoST 9.984
Dawson 10.072
Bjellqvist 9.823
Wikipedia 10.306
Rodwell 10.248
Grimsley 10.189
Solomon 10.145
Lehninger 10.116
Nozaki 9.692
DTASelect 9.823
Thurlkill 9.867
EMBOSS 10.175
Sillero 9.97
Patrickios 7.497
IPC_peptide 10.131
IPC2_peptide 8.624
IPC2.peptide.svr19 8.574
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
60
0
60
12606
38
1528
210.1
23.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.346 ± 0.608
0.555 ± 0.099
6.497 ± 0.23
7.203 ± 0.468
3.887 ± 0.238
6.426 ± 0.486
1.325 ± 0.174
6.87 ± 0.374
8.774 ± 0.42
8.25 ± 0.317
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.618 ± 0.246
6.092 ± 0.201
2.808 ± 0.164
3.673 ± 0.226
3.459 ± 0.249
6.735 ± 0.436
7.124 ± 0.425
6.084 ± 0.222
1.198 ± 0.124
4.077 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here