Fowl aviadenovirus C
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2VJF5|F2VJF5_9ADEN ORF0 OS=Fowl aviadenovirus C OX=190063 PE=4 SV=1
MM1 pKa = 7.7 SLEE4 pKa = 4.52 TEE6 pKa = 4.16 CHH8 pKa = 6.44 APLQYY13 pKa = 10.85 ISMLSFDD20 pKa = 5.25 DD21 pKa = 4.26 LLAAAGPPDD30 pKa = 3.92 YY31 pKa = 11.09 SPEE34 pKa = 4.18 EE35 pKa = 4.06 NQEE38 pKa = 4.0 TPPIEE43 pKa = 4.37 TIEE46 pKa = 4.24 VGDD49 pKa = 3.65 IMAEE53 pKa = 3.72 LGIPIEE59 pKa = 4.72 GPPTSPSDD67 pKa = 3.52 SSSSLDD73 pKa = 3.3 SVLFSGVDD81 pKa = 3.83 LYY83 pKa = 11.61 DD84 pKa = 3.97 LDD86 pKa = 3.96 YY87 pKa = 10.96 TIVFSRR93 pKa = 11.84 LRR95 pKa = 11.84 EE96 pKa = 3.9 FWQSHH101 pKa = 4.12 GAYY104 pKa = 9.28 LKK106 pKa = 9.53 TVASLEE112 pKa = 4.04 CMQNDD117 pKa = 3.53 RR118 pKa = 11.84 KK119 pKa = 10.15 FQEE122 pKa = 4.95 AYY124 pKa = 9.4 CSLVRR129 pKa = 11.84 MHH131 pKa = 6.99 AVSEE135 pKa = 4.2 DD136 pKa = 3.47 AKK138 pKa = 10.72 EE139 pKa = 4.03 HH140 pKa = 6.31 LNEE143 pKa = 4.65 LLLDD147 pKa = 3.62 EE148 pKa = 5.09 TNYY151 pKa = 8.96 QHH153 pKa = 7.46 CEE155 pKa = 3.96 PLNDD159 pKa = 3.65 MLDD162 pKa = 3.71 LGFRR166 pKa = 11.84 WLNDD170 pKa = 3.33 LKK172 pKa = 11.03 GGIMEE177 pKa = 4.55 WCMDD181 pKa = 3.75 TALDD185 pKa = 3.88 RR186 pKa = 11.84 ASKK189 pKa = 10.65 VMPLTDD195 pKa = 3.67 YY196 pKa = 11.04 QPQQ199 pKa = 3.17
Molecular weight: 22.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.812
IPC2_protein 4.062
IPC_protein 4.037
Toseland 3.846
ProMoST 4.164
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.897
Rodwell 3.859
Grimsley 3.745
Solomon 3.999
Lehninger 3.948
Nozaki 4.113
DTASelect 4.304
Thurlkill 3.872
EMBOSS 3.923
Sillero 4.151
Patrickios 1.214
IPC_peptide 3.999
IPC2_peptide 4.126
IPC2.peptide.svr19 4.018
Protein with the highest isoelectric point:
>tr|F2VJH3|F2VJH3_9ADEN PVI OS=Fowl aviadenovirus C OX=190063 GN=pVI PE=4 SV=1
MM1 pKa = 7.68 SILISPNNNTGWGMRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 SRR20 pKa = 11.84 SSSMRR25 pKa = 11.84 GVGMRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 ARR34 pKa = 11.84 PLTLRR39 pKa = 11.84 SLLGLGTRR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 GSRR53 pKa = 11.84 RR54 pKa = 11.84 SRR56 pKa = 11.84 PRR58 pKa = 11.84 TTSRR62 pKa = 11.84 LVVVRR67 pKa = 11.84 TRR69 pKa = 11.84 TSSMRR74 pKa = 11.84 RR75 pKa = 11.84 RR76 pKa = 11.84 RR77 pKa = 3.5
Molecular weight: 9.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.454
IPC2_protein 11.608
IPC_protein 13.217
Toseland 13.378
ProMoST 13.876
Dawson 13.378
Bjellqvist 13.378
Wikipedia 13.861
Rodwell 12.881
Grimsley 13.422
Solomon 13.876
Lehninger 13.773
Nozaki 13.378
DTASelect 13.378
Thurlkill 13.378
EMBOSS 13.876
Sillero 13.378
Patrickios 12.603
IPC_peptide 13.89
IPC2_peptide 12.866
IPC2.peptide.svr19 9.484
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
36
1
37
12476
45
1251
337.2
37.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.382 ± 0.378
1.916 ± 0.278
5.418 ± 0.272
5.683 ± 0.492
4.328 ± 0.258
6.332 ± 0.465
2.461 ± 0.243
4.304 ± 0.229
3.719 ± 0.323
8.913 ± 0.31
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.621 ± 0.166
4.352 ± 0.381
6.917 ± 0.269
4.048 ± 0.263
6.789 ± 0.53
6.813 ± 0.353
6.549 ± 0.58
6.084 ± 0.325
1.347 ± 0.104
4.024 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here