Acetobacteraceae bacterium AT-5844

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; unclassified Acetobacteraceae

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5288 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H0A0L9|H0A0L9_9PROT CsaA protein OS=Acetobacteraceae bacterium AT-5844 OX=1054213 GN=HMPREF9946_02360 PE=4 SV=1
MM1 pKa = 8.14PDD3 pKa = 5.45LITLDD8 pKa = 3.77KK9 pKa = 11.16PEE11 pKa = 4.49DD12 pKa = 4.63AIVLHH17 pKa = 6.9PGDD20 pKa = 4.8NIQEE24 pKa = 4.05AVLAGGEE31 pKa = 4.43GAVFWLEE38 pKa = 3.61AGVYY42 pKa = 9.46RR43 pKa = 11.84MQEE46 pKa = 3.77IEE48 pKa = 4.26PLNGQSFYY56 pKa = 10.89GASGAVLNGSRR67 pKa = 11.84LLTDD71 pKa = 4.0FTQIDD76 pKa = 4.1GHH78 pKa = 5.9WVAEE82 pKa = 4.33GQTQEE87 pKa = 4.04GEE89 pKa = 3.93RR90 pKa = 11.84RR91 pKa = 11.84EE92 pKa = 3.95VDD94 pKa = 3.31EE95 pKa = 4.71AAEE98 pKa = 4.54GAQRR102 pKa = 11.84PGYY105 pKa = 9.25PDD107 pKa = 3.05AVYY110 pKa = 11.39VNDD113 pKa = 3.78QPLKK117 pKa = 10.04PVEE120 pKa = 4.29SLDD123 pKa = 3.93DD124 pKa = 3.93LTSGTYY130 pKa = 10.28YY131 pKa = 10.7FDD133 pKa = 3.88YY134 pKa = 11.26DD135 pKa = 3.11EE136 pKa = 4.66DD137 pKa = 5.01RR138 pKa = 11.84IYY140 pKa = 11.03FGDD143 pKa = 4.23DD144 pKa = 3.2PNGQTVEE151 pKa = 4.18AAVGTHH157 pKa = 7.2AIASTADD164 pKa = 3.26NVTVSGLTVEE174 pKa = 5.61KK175 pKa = 10.57YY176 pKa = 7.68ATPTQFAAITGSGEE190 pKa = 3.55NWIVSNNEE198 pKa = 2.84VRR200 pKa = 11.84LNYY203 pKa = 10.37GVGISVGSDD212 pKa = 3.0GQILNNFVHH221 pKa = 7.73DD222 pKa = 4.27NGQMGLDD229 pKa = 3.21AGGTNILVEE238 pKa = 4.35GNEE241 pKa = 3.96IASNGFWSGIDD252 pKa = 3.6VFWEE256 pKa = 4.39GGGAKK261 pKa = 8.79FTEE264 pKa = 4.27TDD266 pKa = 3.45GLVVRR271 pKa = 11.84NNYY274 pKa = 9.88SHH276 pKa = 7.67DD277 pKa = 3.77NNGYY281 pKa = 9.17GLWTDD286 pKa = 3.02IDD288 pKa = 4.41NINTVYY294 pKa = 10.08EE295 pKa = 4.4GNRR298 pKa = 11.84IEE300 pKa = 4.18YY301 pKa = 10.54NSGGGINHH309 pKa = 7.43EE310 pKa = 3.87ISYY313 pKa = 10.26AASIHH318 pKa = 7.18DD319 pKa = 3.66NTFVGNGGNGLTWLWGSAIQVQNSQDD345 pKa = 3.42VEE347 pKa = 4.1IFNNFIDD354 pKa = 4.47ASSGGNGIGLIQQDD368 pKa = 3.69RR369 pKa = 11.84GEE371 pKa = 4.48GANGPWITVNNNVHH385 pKa = 7.26DD386 pKa = 4.08NTLILSDD393 pKa = 3.69MSGVGAVADD402 pKa = 3.95YY403 pKa = 11.37DD404 pKa = 4.27EE405 pKa = 4.49EE406 pKa = 4.6TMLSGGNVFNNNDD419 pKa = 3.57YY420 pKa = 11.03QVLDD424 pKa = 3.66VEE426 pKa = 4.51NDD428 pKa = 3.06QFAFGEE434 pKa = 4.87FYY436 pKa = 10.72TFEE439 pKa = 5.33DD440 pKa = 3.9FTKK443 pKa = 10.69AANQEE448 pKa = 4.06AGGTLVFAA456 pKa = 5.68

Molecular weight: 49.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H0A5U5|H0A5U5_9PROT Aminotransferase class I/II OS=Acetobacteraceae bacterium AT-5844 OX=1054213 GN=HMPREF9946_04200 PE=4 SV=1
MM1 pKa = 7.27TGIAAHH7 pKa = 6.86IAAAITRR14 pKa = 11.84RR15 pKa = 11.84GARR18 pKa = 11.84SSRR21 pKa = 11.84GRR23 pKa = 11.84RR24 pKa = 11.84GRR26 pKa = 11.84VAARR30 pKa = 11.84LLLRR34 pKa = 11.84LAAGLGLDD42 pKa = 3.68LLTRR46 pKa = 11.84FLLGAGSGFLSGAPLFLSLARR67 pKa = 11.84GLFRR71 pKa = 11.84RR72 pKa = 11.84AAQHH76 pKa = 5.84QRR78 pKa = 11.84LTLTRR83 pKa = 11.84LALTLGFQAAMLLQRR98 pKa = 11.84AQAGRR103 pKa = 11.84LLTLVQGAVAGSVGALRR120 pKa = 11.84ASGRR124 pKa = 11.84ALRR127 pKa = 11.84AGRR130 pKa = 11.84GALL133 pKa = 3.52

Molecular weight: 13.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5288

0

5288

1570546

28

2255

297.0

31.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.849 ± 0.054

0.829 ± 0.009

4.97 ± 0.026

5.664 ± 0.03

3.366 ± 0.02

9.079 ± 0.035

2.03 ± 0.017

4.348 ± 0.023

2.309 ± 0.025

10.936 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.562 ± 0.017

2.24 ± 0.02

6.005 ± 0.033

3.283 ± 0.02

7.88 ± 0.032

4.989 ± 0.026

5.099 ± 0.023

7.228 ± 0.026

1.467 ± 0.015

1.869 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski