Roseburia sp. CAG:309

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; environmental samples

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2223 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6YWA9|R6YWA9_9FIRM Subtilisin-like serine protease OS=Roseburia sp. CAG:309 OX=1262945 GN=BN600_01888 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 10.26KK3 pKa = 10.36KK4 pKa = 10.15IVLCIVLAGLIFSLAGCAEE23 pKa = 4.26EE24 pKa = 5.31AGQPEE29 pKa = 4.7AGVQQEE35 pKa = 3.92NSAVSEE41 pKa = 4.34EE42 pKa = 4.28EE43 pKa = 3.98FAGDD47 pKa = 3.83GAWTILVYY55 pKa = 10.99LCGTDD60 pKa = 4.05LEE62 pKa = 4.63SGYY65 pKa = 11.12GAATMDD71 pKa = 4.11LGEE74 pKa = 4.25MLDD77 pKa = 3.99RR78 pKa = 11.84DD79 pKa = 3.9FGSQVRR85 pKa = 11.84FVVQTGGCTQWQNEE99 pKa = 4.85VIPEE103 pKa = 3.88DD104 pKa = 4.25AIARR108 pKa = 11.84YY109 pKa = 9.31EE110 pKa = 4.14IINGDD115 pKa = 3.44MQEE118 pKa = 3.97ISRR121 pKa = 11.84MDD123 pKa = 3.79QANMAEE129 pKa = 4.46STTLQDD135 pKa = 3.69FVTFGMEE142 pKa = 3.72NYY144 pKa = 10.31GNNKK148 pKa = 9.69LGLILWDD155 pKa = 3.55HH156 pKa = 6.68GGGSVAGVCLDD167 pKa = 3.79EE168 pKa = 5.73NYY170 pKa = 9.0GTDD173 pKa = 3.45TLDD176 pKa = 3.63LSEE179 pKa = 4.99IRR181 pKa = 11.84QALDD185 pKa = 4.25GIPRR189 pKa = 11.84KK190 pKa = 9.99FNFIGYY196 pKa = 7.56DD197 pKa = 3.18ACLMGSVEE205 pKa = 4.24NAKK208 pKa = 9.34MLAPFANYY216 pKa = 9.21MIGSEE221 pKa = 4.13EE222 pKa = 4.27LEE224 pKa = 4.47SGGGWDD230 pKa = 3.62YY231 pKa = 11.87AAIGAYY237 pKa = 9.46LQQNPGAPAADD248 pKa = 4.03LGKK251 pKa = 10.08EE252 pKa = 3.96ICDD255 pKa = 3.49SFYY258 pKa = 11.16KK259 pKa = 10.4SCEE262 pKa = 3.83ALGEE266 pKa = 4.16EE267 pKa = 4.63STATLSVTDD276 pKa = 4.04LSKK279 pKa = 10.53IQKK282 pKa = 9.52VCDD285 pKa = 3.55EE286 pKa = 4.66FNTVAEE292 pKa = 4.33TMKK295 pKa = 10.93GSTEE299 pKa = 3.67NMEE302 pKa = 4.53AFGEE306 pKa = 4.18IAKK309 pKa = 10.44GIRR312 pKa = 11.84KK313 pKa = 7.93AQNYY317 pKa = 8.88GGNTPSEE324 pKa = 5.03GYY326 pKa = 11.13SNMVDD331 pKa = 3.49LGDD334 pKa = 3.65MAKK337 pKa = 10.59NIAGTVDD344 pKa = 3.62TSALQAAIAEE354 pKa = 4.16AVIYY358 pKa = 8.27QVKK361 pKa = 10.45GEE363 pKa = 3.98NRR365 pKa = 11.84QNANGLSVYY374 pKa = 10.48YY375 pKa = 9.37PLQIEE380 pKa = 4.5DD381 pKa = 3.96EE382 pKa = 4.58EE383 pKa = 4.21EE384 pKa = 4.0LKK386 pKa = 10.15TFEE389 pKa = 4.8TICVSDD395 pKa = 4.9PYY397 pKa = 10.91TDD399 pKa = 5.4FIGTMVYY406 pKa = 10.38GAANGSIEE414 pKa = 4.38EE415 pKa = 4.15YY416 pKa = 10.53SGEE419 pKa = 4.25NDD421 pKa = 2.7WSAADD426 pKa = 3.61QSGHH430 pKa = 5.88GLGLVTAEE438 pKa = 3.99QNQIQIAEE446 pKa = 4.16EE447 pKa = 3.58QHH449 pKa = 6.76LNDD452 pKa = 4.13DD453 pKa = 4.14GYY455 pKa = 9.69YY456 pKa = 8.88TFTIAEE462 pKa = 4.01EE463 pKa = 4.13SLPNVASVQYY473 pKa = 10.2NLYY476 pKa = 10.04MKK478 pKa = 10.56FDD480 pKa = 4.68DD481 pKa = 4.38EE482 pKa = 4.75TPLVYY487 pKa = 10.55LGSDD491 pKa = 2.57NWLDD495 pKa = 3.72YY496 pKa = 11.33DD497 pKa = 4.04DD498 pKa = 3.9QTGVVTDD505 pKa = 3.91TFQGYY510 pKa = 7.73WPALPDD516 pKa = 3.77GSLLSMYY523 pKa = 10.15VVEE526 pKa = 4.48EE527 pKa = 4.42SEE529 pKa = 4.98DD530 pKa = 3.6YY531 pKa = 10.99TIFSSPIVLNGRR543 pKa = 11.84EE544 pKa = 4.1TNLRR548 pKa = 11.84FAYY551 pKa = 10.28LYY553 pKa = 11.23GEE555 pKa = 4.34TEE557 pKa = 5.62DD558 pKa = 3.98DD559 pKa = 4.8GEE561 pKa = 4.23WLVLGAWDD569 pKa = 6.14GIDD572 pKa = 4.77DD573 pKa = 5.97DD574 pKa = 5.51SGQADD579 pKa = 3.43KK580 pKa = 11.42EE581 pKa = 4.03MTEE584 pKa = 3.87IQKK587 pKa = 10.96GDD589 pKa = 4.07VIRR592 pKa = 11.84PIYY595 pKa = 10.36VVVDD599 pKa = 3.52QEE601 pKa = 4.71SGDD604 pKa = 3.6TTEE607 pKa = 4.81IKK609 pKa = 10.27GDD611 pKa = 3.31KK612 pKa = 10.56YY613 pKa = 10.61RR614 pKa = 11.84VKK616 pKa = 10.84KK617 pKa = 10.41NFNITEE623 pKa = 4.19PKK625 pKa = 10.28LPEE628 pKa = 3.89GEE630 pKa = 4.4YY631 pKa = 10.46YY632 pKa = 10.84YY633 pKa = 11.43SFGIDD638 pKa = 3.7DD639 pKa = 5.25LYY641 pKa = 11.67GSTKK645 pKa = 10.6YY646 pKa = 10.82LDD648 pKa = 5.57LINFWVNKK656 pKa = 8.42KK657 pKa = 10.67GKK659 pKa = 10.29VEE661 pKa = 3.95LSYY664 pKa = 11.68

Molecular weight: 72.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6YFX9|R6YFX9_9FIRM Uncharacterized protein OS=Roseburia sp. CAG:309 OX=1262945 GN=BN600_01208 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.02KK10 pKa = 9.86SRR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.67GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.05VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2223

0

2223

679977

29

1929

305.9

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.301 ± 0.05

1.542 ± 0.022

5.519 ± 0.039

7.823 ± 0.066

4.104 ± 0.041

6.887 ± 0.048

1.722 ± 0.024

7.21 ± 0.043

7.65 ± 0.051

8.589 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.105 ± 0.027

4.262 ± 0.033

3.258 ± 0.031

3.334 ± 0.03

4.249 ± 0.038

5.666 ± 0.042

5.596 ± 0.048

7.119 ± 0.044

0.89 ± 0.021

4.169 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski