Amino acid dipepetide frequency for Roseburia sp. CAG:309

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.762AlaAla: 6.762 ± 0.125	1.037AlaCys: 1.037 ± 0.041	4.071AlaAsp: 4.071 ± 0.075	5.243AlaGlu: 5.243 ± 0.111	3.093AlaPhe: 3.093 ± 0.074	5.781AlaGly: 5.781 ± 0.101	1.132AlaHis: 1.132 ± 0.045	4.847AlaIle: 4.847 ± 0.099	5.849AlaLys: 5.849 ± 0.085	6.531AlaLeu: 6.531 ± 0.116	2.319AlaMet: 2.319 ± 0.059	2.674AlaAsn: 2.674 ± 0.071	2.018AlaPro: 2.018 ± 0.063	2.152AlaGln: 2.152 ± 0.059	2.725AlaArg: 2.725 ± 0.067	4.078AlaSer: 4.078 ± 0.084	3.49AlaThr: 3.49 ± 0.082	5.968AlaVal: 5.968 ± 0.097	0.584AlaTrp: 0.584 ± 0.03	2.652AlaTyr: 2.652 ± 0.068	0.001AlaXaa: 0.001 ± 0.002
Cys	1.0CysAla: 1.0 ± 0.037	0.25CysCys: 0.25 ± 0.021	0.91CysAsp: 0.91 ± 0.039	1.024CysGlu: 1.024 ± 0.039	0.731CysPhe: 0.731 ± 0.037	1.454CysGly: 1.454 ± 0.051	0.372CysHis: 0.372 ± 0.026	1.082CysIle: 1.082 ± 0.046	1.007CysLys: 1.007 ± 0.04	1.281CysLeu: 1.281 ± 0.043	0.435CysMet: 0.435 ± 0.025	0.61CysAsn: 0.61 ± 0.034	0.629CysPro: 0.629 ± 0.034	0.438CysGln: 0.438 ± 0.025	0.712CysArg: 0.712 ± 0.039	0.875CysSer: 0.875 ± 0.039	0.799CysThr: 0.799 ± 0.036	1.027CysVal: 1.027 ± 0.04	0.116CysTrp: 0.116 ± 0.013	0.662CysTyr: 0.662 ± 0.031	0.0CysXaa: 0.0 ± 0.0
Asp	4.038AspAla: 4.038 ± 0.07	0.831AspCys: 0.831 ± 0.033	2.977AspAsp: 2.977 ± 0.086	4.493AspGlu: 4.493 ± 0.089	2.557AspPhe: 2.557 ± 0.061	4.324AspGly: 4.324 ± 0.084	0.868AspHis: 0.868 ± 0.047	4.396AspIle: 4.396 ± 0.102	3.84AspLys: 3.84 ± 0.077	4.563AspLeu: 4.563 ± 0.085	1.668AspMet: 1.668 ± 0.061	2.132AspAsn: 2.132 ± 0.061	1.674AspPro: 1.674 ± 0.049	1.391AspGln: 1.391 ± 0.042	2.162AspArg: 2.162 ± 0.063	3.016AspSer: 3.016 ± 0.069	3.09AspThr: 3.09 ± 0.062	3.907AspVal: 3.907 ± 0.083	0.596AspTrp: 0.596 ± 0.035	2.669AspTyr: 2.669 ± 0.071	0.0AspXaa: 0.0 ± 0.0
Glu	5.305GluAla: 5.305 ± 0.101	0.859GluCys: 0.859 ± 0.03	4.258GluAsp: 4.258 ± 0.089	7.871GluGlu: 7.871 ± 0.161	2.535GluPhe: 2.535 ± 0.064	4.463GluGly: 4.463 ± 0.088	1.291GluHis: 1.291 ± 0.046	5.866GluIle: 5.866 ± 0.108	7.849GluLys: 7.849 ± 0.128	6.262GluLeu: 6.262 ± 0.114	2.615GluMet: 2.615 ± 0.064	4.275GluAsn: 4.275 ± 0.088	2.029GluPro: 2.029 ± 0.061	3.081GluGln: 3.081 ± 0.077	3.324GluArg: 3.324 ± 0.099	3.399GluSer: 3.399 ± 0.086	4.26GluThr: 4.26 ± 0.093	4.899GluVal: 4.899 ± 0.101	0.672GluTrp: 0.672 ± 0.036	3.113GluTyr: 3.113 ± 0.068	0.001GluXaa: 0.001 ± 0.001
Phe	3.093PheAla: 3.093 ± 0.074	0.835PheCys: 0.835 ± 0.044	2.632PheAsp: 2.632 ± 0.065	2.844PheGlu: 2.844 ± 0.065	1.938PhePhe: 1.938 ± 0.071	2.991PheGly: 2.991 ± 0.061	0.913PheHis: 0.913 ± 0.04	2.485PheIle: 2.485 ± 0.07	1.821PheLys: 1.821 ± 0.053	3.784PheLeu: 3.784 ± 0.091	1.247PheMet: 1.247 ± 0.043	1.365PheAsn: 1.365 ± 0.049	1.412PhePro: 1.412 ± 0.045	1.327PheGln: 1.327 ± 0.049	1.722PheArg: 1.722 ± 0.05	2.99PheSer: 2.99 ± 0.069	2.337PheThr: 2.337 ± 0.065	3.088PheVal: 3.088 ± 0.078	0.396PheTrp: 0.396 ± 0.021	1.821PheTyr: 1.821 ± 0.053	0.0PheXaa: 0.0 ± 0.0
Gly	4.76GlyAla: 4.76 ± 0.097	1.2GlyCys: 1.2 ± 0.048	3.435GlyAsp: 3.435 ± 0.078	4.835GlyGlu: 4.835 ± 0.094	3.009GlyPhe: 3.009 ± 0.078	4.562GlyGly: 4.562 ± 0.103	1.174GlyHis: 1.174 ± 0.047	6.012GlyIle: 6.012 ± 0.109	6.39GlyLys: 6.39 ± 0.111	5.246GlyLeu: 5.246 ± 0.097	2.402GlyMet: 2.402 ± 0.066	3.168GlyAsn: 3.168 ± 0.072	1.172GlyPro: 1.172 ± 0.037	1.909GlyGln: 1.909 ± 0.049	2.85GlyArg: 2.85 ± 0.074	3.859GlySer: 3.859 ± 0.091	4.168GlyThr: 4.168 ± 0.097	4.924GlyVal: 4.924 ± 0.093	0.597GlyTrp: 0.597 ± 0.035	3.191GlyTyr: 3.191 ± 0.065	0.003GlyXaa: 0.003 ± 0.003
His	1.09HisAla: 1.09 ± 0.038	0.341HisCys: 0.341 ± 0.023	0.885HisAsp: 0.885 ± 0.05	1.059HisGlu: 1.059 ± 0.035	0.907HisPhe: 0.907 ± 0.045	1.26HisGly: 1.26 ± 0.056	0.424HisHis: 0.424 ± 0.032	1.338HisIle: 1.338 ± 0.049	1.044HisLys: 1.044 ± 0.037	1.527HisLeu: 1.527 ± 0.054	0.544HisMet: 0.544 ± 0.027	0.763HisAsn: 0.763 ± 0.033	0.785HisPro: 0.785 ± 0.033	0.547HisGln: 0.547 ± 0.029	0.794HisArg: 0.794 ± 0.031	0.897HisSer: 0.897 ± 0.037	0.956HisThr: 0.956 ± 0.037	1.113HisVal: 1.113 ± 0.041	0.171HisTrp: 0.171 ± 0.016	0.778HisTyr: 0.778 ± 0.036	0.0HisXaa: 0.0 ± 0.0
Ile	5.58IleAla: 5.58 ± 0.102	1.334IleCys: 1.334 ± 0.044	3.857IleAsp: 3.857 ± 0.094	4.899IleGlu: 4.899 ± 0.089	2.871IlePhe: 2.871 ± 0.067	4.981IleGly: 4.981 ± 0.095	1.469IleHis: 1.469 ± 0.043	4.466IleIle: 4.466 ± 0.103	4.246IleLys: 4.246 ± 0.079	6.781IleLeu: 6.781 ± 0.121	1.847IleMet: 1.847 ± 0.063	2.59IleAsn: 2.59 ± 0.061	3.143IlePro: 3.143 ± 0.07	2.574IleGln: 2.574 ± 0.054	3.806IleArg: 3.806 ± 0.075	4.693IleSer: 4.693 ± 0.078	4.241IleThr: 4.241 ± 0.09	5.419IleVal: 5.419 ± 0.091	0.575IleTrp: 0.575 ± 0.03	2.727IleTyr: 2.727 ± 0.073	0.003IleXaa: 0.003 ± 0.002
Lys	5.249LysAla: 5.249 ± 0.092	0.878LysCys: 0.878 ± 0.04	4.277LysAsp: 4.277 ± 0.087	7.722LysGlu: 7.722 ± 0.135	2.004LysPhe: 2.004 ± 0.055	4.527LysGly: 4.527 ± 0.093	0.962LysHis: 0.962 ± 0.036	5.569LysIle: 5.569 ± 0.083	8.191LysLys: 8.191 ± 0.168	5.718LysLeu: 5.718 ± 0.11	2.694LysMet: 2.694 ± 0.073	4.021LysAsn: 4.021 ± 0.087	2.225LysPro: 2.225 ± 0.066	2.706LysGln: 2.706 ± 0.071	3.268LysArg: 3.268 ± 0.073	3.496LysSer: 3.496 ± 0.082	4.197LysThr: 4.197 ± 0.089	5.093LysVal: 5.093 ± 0.091	0.674LysTrp: 0.674 ± 0.034	3.027LysTyr: 3.027 ± 0.068	0.001LysXaa: 0.001 ± 0.002
Leu	6.549LeuAla: 6.549 ± 0.113	1.582LeuCys: 1.582 ± 0.05	4.759LeuAsp: 4.759 ± 0.092	6.228LeuGlu: 6.228 ± 0.123	3.76LeuPhe: 3.76 ± 0.097	5.558LeuGly: 5.558 ± 0.097	1.578LeuHis: 1.578 ± 0.045	5.424LeuIle: 5.424 ± 0.106	5.834LeuLys: 5.834 ± 0.094	7.983LeuLeu: 7.983 ± 0.142	2.352LeuMet: 2.352 ± 0.063	3.335LeuAsn: 3.335 ± 0.074	3.413LeuPro: 3.413 ± 0.084	3.153LeuGln: 3.153 ± 0.078	3.769LeuArg: 3.769 ± 0.068	5.788LeuSer: 5.788 ± 0.091	5.025LeuThr: 5.025 ± 0.102	5.681LeuVal: 5.681 ± 0.11	0.765LeuTrp: 0.765 ± 0.04	3.353LeuTyr: 3.353 ± 0.081	0.001LeuXaa: 0.001 ± 0.001
Met	2.39MetAla: 2.39 ± 0.072	0.381MetCys: 0.381 ± 0.025	1.803MetAsp: 1.803 ± 0.052	2.662MetGlu: 2.662 ± 0.065	1.047MetPhe: 1.047 ± 0.034	1.913MetGly: 1.913 ± 0.056	0.406MetHis: 0.406 ± 0.025	2.191MetIle: 2.191 ± 0.059	2.677MetLys: 2.677 ± 0.067	2.582MetLeu: 2.582 ± 0.064	0.957MetMet: 0.957 ± 0.042	1.581MetAsn: 1.581 ± 0.043	1.093MetPro: 1.093 ± 0.036	1.196MetGln: 1.196 ± 0.041	1.335MetArg: 1.335 ± 0.041	1.76MetSer: 1.76 ± 0.053	1.874MetThr: 1.874 ± 0.051	1.944MetVal: 1.944 ± 0.057	0.237MetTrp: 0.237 ± 0.018	1.016MetTyr: 1.016 ± 0.04	0.001MetXaa: 0.001 ± 0.002
Asn	3.09AsnAla: 3.09 ± 0.079	0.701AsnCys: 0.701 ± 0.03	2.197AsnAsp: 2.197 ± 0.059	2.927AsnGlu: 2.927 ± 0.071	1.538AsnPhe: 1.538 ± 0.046	3.357AsnGly: 3.357 ± 0.072	0.785AsnHis: 0.785 ± 0.034	3.175AsnIle: 3.175 ± 0.07	2.672AsnLys: 2.672 ± 0.071	3.563AsnLeu: 3.563 ± 0.074	1.288AsnMet: 1.288 ± 0.047	1.757AsnAsn: 1.757 ± 0.068	1.979AsnPro: 1.979 ± 0.055	1.434AsnGln: 1.434 ± 0.058	1.893AsnArg: 1.893 ± 0.056	2.175AsnSer: 2.175 ± 0.059	2.488AsnThr: 2.488 ± 0.068	3.275AsnVal: 3.275 ± 0.059	0.421AsnTrp: 0.421 ± 0.022	1.902AsnTyr: 1.902 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	2.591ProAla: 2.591 ± 0.067	0.49ProCys: 0.49 ± 0.03	2.154ProAsp: 2.154 ± 0.056	3.287ProGlu: 3.287 ± 0.084	1.491ProPhe: 1.491 ± 0.049	2.178ProGly: 2.178 ± 0.058	0.524ProHis: 0.524 ± 0.03	2.034ProIle: 2.034 ± 0.059	2.102ProLys: 2.102 ± 0.057	2.671ProLeu: 2.671 ± 0.063	0.9ProMet: 0.9 ± 0.038	1.254ProAsn: 1.254 ± 0.047	0.672ProPro: 0.672 ± 0.033	1.121ProGln: 1.121 ± 0.042	0.966ProArg: 0.966 ± 0.038	1.774ProSer: 1.774 ± 0.057	1.7ProThr: 1.7 ± 0.063	2.905ProVal: 2.905 ± 0.062	0.337ProTrp: 0.337 ± 0.024	1.435ProTyr: 1.435 ± 0.052	0.0ProXaa: 0.0 ± 0.0
Gln	2.421GlnAla: 2.421 ± 0.059	0.457GlnCys: 0.457 ± 0.027	1.447GlnAsp: 1.447 ± 0.047	2.619GlnGlu: 2.619 ± 0.063	1.296GlnPhe: 1.296 ± 0.042	2.052GlnGly: 2.052 ± 0.057	0.444GlnHis: 0.444 ± 0.03	2.866GlnIle: 2.866 ± 0.077	3.122GlnLys: 3.122 ± 0.09	2.744GlnLeu: 2.744 ± 0.067	1.344GlnMet: 1.344 ± 0.039	1.697GlnAsn: 1.697 ± 0.054	0.944GlnPro: 0.944 ± 0.043	1.219GlnGln: 1.219 ± 0.045	1.29GlnArg: 1.29 ± 0.05	1.649GlnSer: 1.649 ± 0.05	1.906GlnThr: 1.906 ± 0.052	2.146GlnVal: 2.146 ± 0.06	0.319GlnTrp: 0.319 ± 0.023	1.359GlnTyr: 1.359 ± 0.047	0.0GlnXaa: 0.0 ± 0.0
Arg	2.506ArgAla: 2.506 ± 0.055	0.596ArgCys: 0.596 ± 0.031	2.138ArgAsp: 2.138 ± 0.056	3.665ArgGlu: 3.665 ± 0.094	1.902ArgPhe: 1.902 ± 0.055	2.557ArgGly: 2.557 ± 0.065	0.784ArgHis: 0.784 ± 0.031	3.309ArgIle: 3.309 ± 0.076	3.675ArgLys: 3.675 ± 0.072	3.565ArgLeu: 3.565 ± 0.086	1.519ArgMet: 1.519 ± 0.041	1.854ArgAsn: 1.854 ± 0.059	1.257ArgPro: 1.257 ± 0.044	1.665ArgGln: 1.665 ± 0.054	2.225ArgArg: 2.225 ± 0.075	2.062ArgSer: 2.062 ± 0.054	2.082ArgThr: 2.082 ± 0.06	2.812ArgVal: 2.812 ± 0.067	0.376ArgTrp: 0.376 ± 0.027	1.934ArgTyr: 1.934 ± 0.06	0.003ArgXaa: 0.003 ± 0.002
Ser	4.044SerAla: 4.044 ± 0.094	0.785SerCys: 0.785 ± 0.036	3.171SerAsp: 3.171 ± 0.07	4.147SerGlu: 4.147 ± 0.08	2.515SerPhe: 2.515 ± 0.064	4.703SerGly: 4.703 ± 0.091	1.0SerHis: 1.0 ± 0.042	4.072SerIle: 4.072 ± 0.078	3.725SerLys: 3.725 ± 0.08	5.087SerLeu: 5.087 ± 0.091	1.759SerMet: 1.759 ± 0.053	2.157SerAsn: 2.157 ± 0.057	1.688SerPro: 1.688 ± 0.052	1.849SerGln: 1.849 ± 0.052	2.427SerArg: 2.427 ± 0.064	3.682SerSer: 3.682 ± 0.1	2.746SerThr: 2.746 ± 0.068	4.109SerVal: 4.109 ± 0.091	0.503SerTrp: 0.503 ± 0.028	2.493SerTyr: 2.493 ± 0.06	0.0SerXaa: 0.0 ± 0.0
Thr	4.341ThrAla: 4.341 ± 0.089	0.747ThrCys: 0.747 ± 0.037	3.009ThrAsp: 3.009 ± 0.072	3.891ThrGlu: 3.891 ± 0.08	2.36ThrPhe: 2.36 ± 0.07	4.496ThrGly: 4.496 ± 0.094	0.902ThrHis: 0.902 ± 0.039	4.209ThrIle: 4.209 ± 0.092	3.855ThrLys: 3.855 ± 0.077	5.093ThrLeu: 5.093 ± 0.099	1.616ThrMet: 1.616 ± 0.05	2.152ThrAsn: 2.152 ± 0.065	2.328ThrPro: 2.328 ± 0.071	1.56ThrGln: 1.56 ± 0.052	1.79ThrArg: 1.79 ± 0.059	3.081ThrSer: 3.081 ± 0.069	3.275ThrThr: 3.275 ± 0.091	4.381ThrVal: 4.381 ± 0.106	0.544ThrTrp: 0.544 ± 0.028	2.332ThrTyr: 2.332 ± 0.066	0.0ThrXaa: 0.0 ± 0.0
Val	4.863ValAla: 4.863 ± 0.104	1.291ValCys: 1.291 ± 0.043	4.046ValAsp: 4.046 ± 0.076	4.753ValGlu: 4.753 ± 0.088	3.166ValPhe: 3.166 ± 0.071	4.188ValGly: 4.188 ± 0.084	1.065ValHis: 1.065 ± 0.042	5.383ValIle: 5.383 ± 0.095	5.333ValLys: 5.333 ± 0.098	6.527ValLeu: 6.527 ± 0.1	2.034ValMet: 2.034 ± 0.055	3.027ValAsn: 3.027 ± 0.069	2.581ValPro: 2.581 ± 0.062	2.034ValGln: 2.034 ± 0.057	2.852ValArg: 2.852 ± 0.066	4.674ValSer: 4.674 ± 0.09	4.683ValThr: 4.683 ± 0.105	5.262ValVal: 5.262 ± 0.102	0.574ValTrp: 0.574 ± 0.029	2.853ValTyr: 2.853 ± 0.072	0.003ValXaa: 0.003 ± 0.002
Trp	0.468TrpAla: 0.468 ± 0.023	0.153TrpCys: 0.153 ± 0.016	0.509TrpAsp: 0.509 ± 0.027	0.676TrpGlu: 0.676 ± 0.034	0.441TrpPhe: 0.441 ± 0.025	0.541TrpGly: 0.541 ± 0.029	0.203TrpHis: 0.203 ± 0.015	0.669TrpIle: 0.669 ± 0.038	0.882TrpLys: 0.882 ± 0.038	0.799TrpLeu: 0.799 ± 0.037	0.354TrpMet: 0.354 ± 0.023	0.488TrpAsn: 0.488 ± 0.026	0.178TrpPro: 0.178 ± 0.018	0.388TrpGln: 0.388 ± 0.024	0.372TrpArg: 0.372 ± 0.024	0.447TrpSer: 0.447 ± 0.029	0.407TrpThr: 0.407 ± 0.025	0.441TrpVal: 0.441 ± 0.028	0.1TrpTrp: 0.1 ± 0.012	0.382TrpTyr: 0.382 ± 0.028	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.865TyrAla: 2.865 ± 0.071	0.666TyrCys: 0.666 ± 0.032	2.656TyrAsp: 2.656 ± 0.069	3.381TyrGlu: 3.381 ± 0.088	1.878TyrPhe: 1.878 ± 0.054	2.98TyrGly: 2.98 ± 0.075	0.929TyrHis: 0.929 ± 0.037	2.703TyrIle: 2.703 ± 0.067	2.384TyrLys: 2.384 ± 0.069	3.584TyrLeu: 3.584 ± 0.077	1.146TyrMet: 1.146 ± 0.039	1.718TyrAsn: 1.718 ± 0.055	1.36TyrPro: 1.36 ± 0.043	1.597TyrGln: 1.597 ± 0.052	2.204TyrArg: 2.204 ± 0.056	2.268TyrSer: 2.268 ± 0.062	2.238TyrThr: 2.238 ± 0.071	2.794TyrVal: 2.794 ± 0.063	0.346TyrTrp: 0.346 ± 0.023	1.99TyrTyr: 1.99 ± 0.071	0.001TyrXaa: 0.001 ± 0.002
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.003XaaIle: 0.003 ± 0.002	0.001XaaLys: 0.001 ± 0.002	0.001XaaLeu: 0.001 ± 0.001	0.004XaaMet: 0.004 ± 0.003	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.002	0.001XaaGln: 0.001 ± 0.002	0.001XaaArg: 0.001 ± 0.002	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.002	0.001XaaTrp: 0.001 ± 0.002	0.0XaaTyr: 0.0 ± 0.0	0.034XaaXaa: 0.034 ± 0.01

Statistics based on 2223 proteins (679977 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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