Streptococcus phage IPP42

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SCY7|A0A1S5SCY7_9CAUD Uncharacterized protein OS=Streptococcus phage IPP42 OX=1916181 GN=IPP42_00014 PE=4 SV=1
MM1 pKa = 7.26MEE3 pKa = 4.09EE4 pKa = 4.25LKK6 pKa = 11.0KK7 pKa = 10.73KK8 pKa = 10.5VNAVYY13 pKa = 10.49NWTVEE18 pKa = 4.22DD19 pKa = 4.87GKK21 pKa = 9.82PQPPQQDD28 pKa = 3.04LPQAVKK34 pKa = 10.63EE35 pKa = 4.03RR36 pKa = 11.84VDD38 pKa = 3.95YY39 pKa = 9.78FWEE42 pKa = 4.03MAEE45 pKa = 5.33DD46 pKa = 3.88GMTFMGAMEE55 pKa = 5.67CIFADD60 pKa = 4.73EE61 pKa = 5.02KK62 pKa = 10.3PTDD65 pKa = 3.84YY66 pKa = 11.56DD67 pKa = 4.51LGATKK72 pKa = 10.55DD73 pKa = 3.52WLPKK77 pKa = 10.51SKK79 pKa = 10.64EE80 pKa = 3.52FDD82 pKa = 3.25DD83 pKa = 3.91WVGYY87 pKa = 8.92SPSMAQVVIAVYY99 pKa = 10.34LIYY102 pKa = 10.82GGNN105 pKa = 3.26

Molecular weight: 12.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SCK7|A0A1S5SCK7_9CAUD Putative lipoprotein OS=Streptococcus phage IPP42 OX=1916181 GN=IPP42_00009 PE=4 SV=1
MM1 pKa = 6.99YY2 pKa = 10.58NKK4 pKa = 9.6VYY6 pKa = 10.71HH7 pKa = 6.0KK8 pKa = 8.14TTFGLSDD15 pKa = 3.96LFKK18 pKa = 10.8SSKK21 pKa = 9.54KK22 pKa = 9.68RR23 pKa = 11.84KK24 pKa = 9.59KK25 pKa = 10.28KK26 pKa = 10.11VVNNKK31 pKa = 7.36QQSILASNGKK41 pKa = 8.59KK42 pKa = 7.92QHH44 pKa = 5.19TSKK47 pKa = 10.53EE48 pKa = 4.15YY49 pKa = 10.76KK50 pKa = 9.72EE51 pKa = 4.44AGLVFMVLGAIFLFLFLPLGFFLFITGFITYY82 pKa = 10.14IIGLLTANRR91 pKa = 11.84EE92 pKa = 4.17KK93 pKa = 10.75KK94 pKa = 10.46KK95 pKa = 10.57KK96 pKa = 9.39VEE98 pKa = 4.13NYY100 pKa = 10.0SPQIDD105 pKa = 3.62TTVFRR110 pKa = 11.84DD111 pKa = 3.46DD112 pKa = 3.71FLLMGTNYY120 pKa = 9.65HH121 pKa = 5.56QEE123 pKa = 4.09EE124 pKa = 4.45AEE126 pKa = 4.0IAADD130 pKa = 3.85FLSKK134 pKa = 10.34GVHH137 pKa = 5.87YY138 pKa = 10.29FGKK141 pKa = 10.18DD142 pKa = 3.05NKK144 pKa = 10.24SLKK147 pKa = 10.54SYY149 pKa = 9.93MLEE152 pKa = 4.16TYY154 pKa = 10.38KK155 pKa = 10.58PVYY158 pKa = 9.99KK159 pKa = 10.49YY160 pKa = 11.29NKK162 pKa = 9.23LKK164 pKa = 10.14TVDD167 pKa = 3.38VHH169 pKa = 7.26LLPEE173 pKa = 4.57PSNPHH178 pKa = 6.62DD179 pKa = 4.11KK180 pKa = 11.11NAVKK184 pKa = 10.81VLVNNIFVGYY194 pKa = 8.92LPAMIATQVSSYY206 pKa = 10.61ISNPNYY212 pKa = 10.34RR213 pKa = 11.84YY214 pKa = 10.15DD215 pKa = 4.71AILTGRR221 pKa = 11.84GGPYY225 pKa = 8.45KK226 pKa = 9.86TLNIEE231 pKa = 3.96TEE233 pKa = 4.19RR234 pKa = 11.84VVSRR238 pKa = 11.84KK239 pKa = 9.68SDD241 pKa = 3.19LTYY244 pKa = 11.29YY245 pKa = 11.07LDD247 pKa = 3.57LTVWRR252 pKa = 11.84LAKK255 pKa = 10.37KK256 pKa = 10.27

Molecular weight: 29.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

10967

34

3035

255.0

28.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.684 ± 0.581

0.574 ± 0.112

5.854 ± 0.349

7.796 ± 0.335

3.976 ± 0.218

5.836 ± 0.341

1.486 ± 0.193

6.693 ± 0.261

8.516 ± 0.394

8.444 ± 0.411

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.261 ± 0.312

6.064 ± 0.239

2.553 ± 0.198

4.049 ± 0.287

4.669 ± 0.345

6.83 ± 0.648

6.583 ± 0.417

5.945 ± 0.231

1.514 ± 0.151

3.675 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski