TM7 phylum sp. oral taxon 349
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 963 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A563CXA7|A0A563CXA7_9BACT Uncharacterized protein OS=TM7 phylum sp. oral taxon 349 OX=713051 GN=EUA66_02615 PE=4 SV=1
MM1 pKa = 7.58 EE2 pKa = 5.9 LNPGEE7 pKa = 4.21 RR8 pKa = 11.84 YY9 pKa = 9.08 EE10 pKa = 4.3 VAFDD14 pKa = 3.89 SYY16 pKa = 11.06 TGNLAVGEE24 pKa = 4.25 TVEE27 pKa = 5.95 FYY29 pKa = 11.36 DD30 pKa = 5.13 NNDD33 pKa = 4.51 LICVEE38 pKa = 5.05 LEE40 pKa = 3.97 NLDD43 pKa = 4.49 DD44 pKa = 4.67 SMGNVIRR51 pKa = 11.84 AIGGVAGQEE60 pKa = 4.28 IIMSSGLFSCDD71 pKa = 3.18 HH72 pKa = 6.9 GDD74 pKa = 3.18 GFAIFKK80 pKa = 10.28 ARR82 pKa = 11.84 HH83 pKa = 6.55 DD84 pKa = 5.14 DD85 pKa = 3.64 IQDD88 pKa = 3.31 VSS90 pKa = 3.48
Molecular weight: 9.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.846
IPC_protein 3.795
Toseland 3.592
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.719
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.656
EMBOSS 3.732
Sillero 3.91
Patrickios 1.875
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|A0A563D216|A0A563D216_9BACT Uncharacterized protein OS=TM7 phylum sp. oral taxon 349 OX=713051 GN=EUA66_01090 PE=4 SV=1
MM1 pKa = 7.34 NGTWRR6 pKa = 11.84 WQEE9 pKa = 3.99 LAWDD13 pKa = 4.19 SPATPAVGAAALEE26 pKa = 4.17 RR27 pKa = 11.84 LTTAVEE33 pKa = 3.99 VGPVVLEE40 pKa = 3.71 LRR42 pKa = 11.84 AWAGGAYY49 pKa = 8.59 WLAGCQPGRR58 pKa = 11.84 AVEE61 pKa = 4.28 LKK63 pKa = 10.46 QLLGHH68 pKa = 6.71 HH69 pKa = 6.6 LPARR73 pKa = 11.84 LHH75 pKa = 5.93 RR76 pKa = 11.84 PQRR79 pKa = 11.84 QRR81 pKa = 11.84 LAVGQAVRR89 pKa = 11.84 LQVRR93 pKa = 11.84 GNHH96 pKa = 5.39 VSTSADD102 pKa = 2.7 RR103 pKa = 11.84 VVAATRR109 pKa = 11.84 ALYY112 pKa = 10.42 ACLSGLHH119 pKa = 6.43 EE120 pKa = 4.39 NQQAVVQLLIGRR132 pKa = 11.84 RR133 pKa = 11.84 LPSSSFTGPPAPGWRR148 pKa = 11.84 EE149 pKa = 3.79 LFLGQPAPRR158 pKa = 11.84 SPEE161 pKa = 3.72 RR162 pKa = 11.84 PTRR165 pKa = 11.84 TTSEE169 pKa = 3.7 QHH171 pKa = 5.69 GAAACLRR178 pKa = 11.84 LGHH181 pKa = 6.72 TGTPAQAHH189 pKa = 4.75 QLFSRR194 pKa = 11.84 LLGALRR200 pKa = 11.84 IVEE203 pKa = 4.26 TPRR206 pKa = 11.84 ARR208 pKa = 11.84 FRR210 pKa = 11.84 LSAEE214 pKa = 3.8 APDD217 pKa = 3.51 RR218 pKa = 11.84 LNRR221 pKa = 11.84 VSRR224 pKa = 11.84 PWAWPLRR231 pKa = 11.84 LRR233 pKa = 11.84 SGQLTALCGWPIGEE247 pKa = 4.84 PPLPLLGDD255 pKa = 3.54 LHH257 pKa = 6.83 PRR259 pKa = 11.84 LLPPPASLVRR269 pKa = 11.84 SEE271 pKa = 4.48 RR272 pKa = 11.84 VIGLASAPGCRR283 pKa = 11.84 QPVAIPIRR291 pKa = 11.84 DD292 pKa = 3.75 AVFHH296 pKa = 5.55 THH298 pKa = 7.12 LLGPTGTGKK307 pKa = 8.49 STVLLSLALADD318 pKa = 4.13 VEE320 pKa = 4.12 AHH322 pKa = 6.42 RR323 pKa = 11.84 AVLLLDD329 pKa = 3.97 PKK331 pKa = 11.28 GDD333 pKa = 3.71 LATDD337 pKa = 3.93 LLARR341 pKa = 11.84 VPEE344 pKa = 4.47 GRR346 pKa = 11.84 HH347 pKa = 5.18 EE348 pKa = 4.35 DD349 pKa = 3.68 VVVIDD354 pKa = 4.25 PTNPAPVGLNPLAGPARR371 pKa = 11.84 LAPVTADD378 pKa = 3.31 SLLGTLAALFHH389 pKa = 5.8 EE390 pKa = 4.71 HH391 pKa = 6.02 WGVRR395 pKa = 11.84 TADD398 pKa = 3.6 VLNAALLTLARR409 pKa = 11.84 TPGANLLWLPPLLTDD424 pKa = 3.41 PAFRR428 pKa = 11.84 RR429 pKa = 11.84 RR430 pKa = 11.84 VLDD433 pKa = 4.38 DD434 pKa = 3.31 QDD436 pKa = 5.23 DD437 pKa = 4.09 PLGTGAFWAAYY448 pKa = 6.69 EE449 pKa = 4.15 AKK451 pKa = 10.14 RR452 pKa = 11.84 PQAQAVEE459 pKa = 4.14 IAPVLNKK466 pKa = 9.82 LRR468 pKa = 11.84 QLILRR473 pKa = 11.84 PHH475 pKa = 6.2 LRR477 pKa = 11.84 AVLGQSAPRR486 pKa = 11.84 FTVEE490 pKa = 3.89 EE491 pKa = 4.29 LFTRR495 pKa = 11.84 KK496 pKa = 9.96 RR497 pKa = 11.84 IVVVNLNRR505 pKa = 11.84 GLLGAEE511 pKa = 4.34 AARR514 pKa = 11.84 LLGSLLVGQLWSRR527 pKa = 11.84 LLARR531 pKa = 11.84 QALPPEE537 pKa = 4.28 RR538 pKa = 11.84 RR539 pKa = 11.84 HH540 pKa = 5.23 VVSVYY545 pKa = 10.05 IDD547 pKa = 3.53 EE548 pKa = 4.45 AHH550 pKa = 7.09 DD551 pKa = 4.35 FINGLPGDD559 pKa = 4.03 LSEE562 pKa = 5.58 ALAQARR568 pKa = 11.84 SLGGALHH575 pKa = 7.28 LAHH578 pKa = 7.04 QYY580 pKa = 10.03 RR581 pKa = 11.84 AQLSPAMVQALEE593 pKa = 4.04 TNARR597 pKa = 11.84 NKK599 pKa = 10.16 IYY601 pKa = 10.46 FGLSGTDD608 pKa = 3.21 AAAVAHH614 pKa = 6.72 LAPEE618 pKa = 4.93 LEE620 pKa = 4.22 AQDD623 pKa = 5.27 FIGLPAYY630 pKa = 9.7 HH631 pKa = 7.45 AYY633 pKa = 8.04 ATLMQQGRR641 pKa = 11.84 STGWLSVTTRR651 pKa = 11.84 PAPPARR657 pKa = 11.84 QEE659 pKa = 3.97 PASLYY664 pKa = 9.99 AASHH668 pKa = 4.74 QRR670 pKa = 11.84 YY671 pKa = 8.3 GVPATRR677 pKa = 11.84 TEE679 pKa = 3.89 QQLINLTKK687 pKa = 9.45 PSHH690 pKa = 6.69 HH691 pKa = 6.54 ATQPPDD697 pKa = 3.71 SPAKK701 pKa = 10.11 PSTGSAAPDD710 pKa = 3.26 QPGPIGRR717 pKa = 11.84 RR718 pKa = 11.84 RR719 pKa = 11.84 RR720 pKa = 3.46
Molecular weight: 77.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.472
IPC_protein 10.599
Toseland 10.526
ProMoST 10.57
Dawson 10.657
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.584
Grimsley 10.716
Solomon 10.833
Lehninger 10.789
Nozaki 10.54
DTASelect 10.467
Thurlkill 10.555
EMBOSS 10.965
Sillero 10.599
Patrickios 10.189
IPC_peptide 10.833
IPC2_peptide 9.721
IPC2.peptide.svr19 8.734
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
963
0
963
276238
37
1281
286.9
31.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.471 ± 0.094
0.797 ± 0.023
5.829 ± 0.059
5.767 ± 0.073
3.61 ± 0.05
6.691 ± 0.08
2.084 ± 0.038
6.667 ± 0.069
5.664 ± 0.08
9.022 ± 0.085
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.32 ± 0.034
4.166 ± 0.059
3.867 ± 0.059
3.864 ± 0.048
5.984 ± 0.069
6.373 ± 0.076
6.019 ± 0.064
7.338 ± 0.084
1.063 ± 0.031
3.402 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here