Rhodobacter sp. SGA-6-6

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodobacter; unclassified Rhodobacter

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4072 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M1MI08|A0A6M1MI08_9RHOB YciI family protein OS=Rhodobacter sp. SGA-6-6 OX=2710882 GN=G5B31_02870 PE=3 SV=1
MM1 pKa = 8.07RR2 pKa = 11.84ILALTSACLLAFSTPVLADD21 pKa = 3.44PAVGLGLSFSFGGGQSQTGIGLRR44 pKa = 11.84VFSDD48 pKa = 3.79DD49 pKa = 4.66EE50 pKa = 4.39EE51 pKa = 5.97DD52 pKa = 3.97SFAASAGLDD61 pKa = 3.56YY62 pKa = 11.08IFGSGAWRR70 pKa = 11.84GTVGGAYY77 pKa = 10.39LGDD80 pKa = 3.58NSYY83 pKa = 11.55VGLDD87 pKa = 3.04VGLNFNGGGVNYY99 pKa = 9.74GISAGAVNTEE109 pKa = 4.44DD110 pKa = 3.33KK111 pKa = 10.68AAPAVTPPPGPPPVV125 pKa = 3.38

Molecular weight: 12.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M1MGW4|A0A6M1MGW4_9RHOB HutD family protein OS=Rhodobacter sp. SGA-6-6 OX=2710882 GN=G5B31_00995 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.26LVRR12 pKa = 11.84SRR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.43GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34GGRR28 pKa = 11.84LVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4072

0

4072

1265259

33

2811

310.7

33.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.842 ± 0.059

0.863 ± 0.011

5.489 ± 0.034

5.935 ± 0.039

3.544 ± 0.025

9.21 ± 0.048

1.997 ± 0.021

4.594 ± 0.027

2.772 ± 0.031

10.64 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.646 ± 0.016

2.105 ± 0.021

5.674 ± 0.028

2.903 ± 0.019

7.396 ± 0.048

4.515 ± 0.025

5.016 ± 0.022

7.32 ± 0.032

1.511 ± 0.017

2.028 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski