Escherichia phage UAB_Phi78

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; Zindervirus; Escherichia virus UAB78

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1E361|M1E361_9CAUD Uncharacterized protein OS=Escherichia phage UAB_Phi78 OX=979726 GN=Phi78p11 PE=4 SV=1
MM1 pKa = 7.59SYY3 pKa = 11.74SNMTYY8 pKa = 11.03ANVYY12 pKa = 7.95NHH14 pKa = 7.47AYY16 pKa = 10.34SLLDD20 pKa = 3.43EE21 pKa = 4.71YY22 pKa = 10.87IRR24 pKa = 11.84YY25 pKa = 9.8EE26 pKa = 4.09DD27 pKa = 3.59VRR29 pKa = 11.84DD30 pKa = 3.57YY31 pKa = 11.85DD32 pKa = 3.63EE33 pKa = 4.78ATDD36 pKa = 4.77KK37 pKa = 10.97IHH39 pKa = 6.68EE40 pKa = 4.49AADD43 pKa = 3.49NAVPVYY49 pKa = 10.35YY50 pKa = 10.52KK51 pKa = 10.67DD52 pKa = 3.16IFSVMASGGIDD63 pKa = 3.57HH64 pKa = 6.87EE65 pKa = 4.96FEE67 pKa = 4.99DD68 pKa = 4.97SGLIPDD74 pKa = 4.29TKK76 pKa = 10.82DD77 pKa = 2.92VTRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 9.25EE88 pKa = 4.09QLTIDD93 pKa = 4.19LYY95 pKa = 11.38EE96 pKa = 4.31VVADD100 pKa = 4.67LLNEE104 pKa = 3.89YY105 pKa = 10.4LEE107 pKa = 4.28EE108 pKa = 4.44VEE110 pKa = 4.98AEE112 pKa = 3.85EE113 pKa = 5.67DD114 pKa = 3.76EE115 pKa = 4.55EE116 pKa = 4.78EE117 pKa = 4.25EE118 pKa = 4.29

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1E3Q8|M1E3Q8_9CAUD Uncharacterized protein OS=Escherichia phage UAB_Phi78 OX=979726 GN=Phi78p08 PE=4 SV=1
MM1 pKa = 6.89TRR3 pKa = 11.84LYY5 pKa = 10.94KK6 pKa = 10.39DD7 pKa = 3.98DD8 pKa = 4.31SSGAYY13 pKa = 7.65YY14 pKa = 8.77TLCGRR19 pKa = 11.84TLILLNGATGRR30 pKa = 11.84RR31 pKa = 11.84LISYY35 pKa = 8.23LATHH39 pKa = 7.33RR40 pKa = 11.84ACIHH44 pKa = 6.47SGLQLVGNNFKK55 pKa = 10.7RR56 pKa = 11.84KK57 pKa = 8.14QLL59 pKa = 3.61

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

13292

20

1270

229.2

25.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.163 ± 0.603

1.113 ± 0.16

5.951 ± 0.197

7.094 ± 0.291

3.408 ± 0.144

7.892 ± 0.429

2.129 ± 0.237

5.537 ± 0.278

6.38 ± 0.265

7.945 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.273 ± 0.14

4.063 ± 0.166

3.476 ± 0.227

4.078 ± 0.227

5.642 ± 0.262

5.846 ± 0.273

5.296 ± 0.264

6.861 ± 0.303

1.482 ± 0.136

3.37 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski