Ruminococcaceae bacterium YRB3002

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; unclassified Oscillospiraceae

Average proteome isoelectric point is 5.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2644 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G4P2K9|A0A1G4P2K9_9FIRM Selenocysteine lyase/Cysteine desulfurase OS=Ruminococcaceae bacterium YRB3002 OX=1520819 GN=SAMN02910456_00060 PE=3 SV=1
MM1 pKa = 7.48INSKK5 pKa = 10.51KK6 pKa = 10.1ILAVVLAGTLALSCAACTSINKK28 pKa = 9.64EE29 pKa = 4.06DD30 pKa = 3.99VIDD33 pKa = 4.1AAEE36 pKa = 4.16SFAKK40 pKa = 10.32AVAALDD46 pKa = 3.79GKK48 pKa = 11.36KK49 pKa = 9.02MLKK52 pKa = 10.27QIDD55 pKa = 4.36DD56 pKa = 3.93IKK58 pKa = 11.11DD59 pKa = 3.42SKK61 pKa = 11.47ADD63 pKa = 3.54QIKK66 pKa = 10.81NKK68 pKa = 10.51LSMSDD73 pKa = 3.98LDD75 pKa = 3.94PDD77 pKa = 3.42EE78 pKa = 5.41AAVKK82 pKa = 10.23KK83 pKa = 10.72AIADD87 pKa = 4.2TITYY91 pKa = 9.51EE92 pKa = 3.82VDD94 pKa = 3.22EE95 pKa = 5.77DD96 pKa = 4.14SVQAGKK102 pKa = 11.04SNDD105 pKa = 3.33EE106 pKa = 4.0ATVDD110 pKa = 3.75VVFTMVDD117 pKa = 3.44YY118 pKa = 11.51AKK120 pKa = 10.9VLTDD124 pKa = 6.41DD125 pKa = 3.79ITTKK129 pKa = 11.29DD130 pKa = 4.08DD131 pKa = 3.99MIDD134 pKa = 3.78AIKK137 pKa = 10.47DD138 pKa = 3.53SKK140 pKa = 9.22KK141 pKa = 7.75TKK143 pKa = 9.13EE144 pKa = 3.99YY145 pKa = 10.26EE146 pKa = 4.1VTFEE150 pKa = 4.9LVADD154 pKa = 4.72DD155 pKa = 4.83GDD157 pKa = 3.79WLIAEE162 pKa = 4.55DD163 pKa = 3.96TLGEE167 pKa = 4.4LGSLYY172 pKa = 10.96DD173 pKa = 3.74FLDD176 pKa = 3.92YY177 pKa = 10.92EE178 pKa = 4.38LSIGGASGNVIDD190 pKa = 5.61MIDD193 pKa = 3.46HH194 pKa = 6.28TSWWMSGVDD203 pKa = 4.41DD204 pKa = 5.01NYY206 pKa = 11.59TNATYY211 pKa = 10.34IEE213 pKa = 4.13FDD215 pKa = 3.21VWFTDD220 pKa = 3.42NPNVSVYY227 pKa = 8.63YY228 pKa = 10.16TVDD231 pKa = 3.13KK232 pKa = 11.17DD233 pKa = 3.58GSQVYY238 pKa = 9.87QSDD241 pKa = 3.84VQTFSGSYY249 pKa = 10.23FEE251 pKa = 5.81AKK253 pKa = 10.68YY254 pKa = 10.76SDD256 pKa = 3.67EE257 pKa = 5.2QNAQMNNGYY266 pKa = 9.29IAAGTYY272 pKa = 9.96KK273 pKa = 10.15IVVFTADD280 pKa = 3.12GTQIADD286 pKa = 3.23GSTTVTVDD294 pKa = 2.62ATTPTTTTSGSDD306 pKa = 3.04TSTEE310 pKa = 4.46FYY312 pKa = 10.05TIHH315 pKa = 7.32DD316 pKa = 4.19GSFADD321 pKa = 3.79IKK323 pKa = 10.94EE324 pKa = 4.2IGWWDD329 pKa = 3.59YY330 pKa = 11.84GVDD333 pKa = 4.39DD334 pKa = 6.29DD335 pKa = 6.58GDD337 pKa = 3.96GEE339 pKa = 4.3NDD341 pKa = 3.82HH342 pKa = 7.45GSMAQDD348 pKa = 3.19GVYY351 pKa = 10.74CIDD354 pKa = 3.48TEE356 pKa = 4.35IIAFSIEE363 pKa = 4.12LNKK366 pKa = 10.55EE367 pKa = 3.45ADD369 pKa = 4.34DD370 pKa = 3.94IYY372 pKa = 10.63YY373 pKa = 10.45AYY375 pKa = 10.9YY376 pKa = 10.1FLPGEE381 pKa = 4.86DD382 pKa = 3.26VDD384 pKa = 5.45ASNIDD389 pKa = 3.49YY390 pKa = 11.03SNPTFADD397 pKa = 4.16TISATSYY404 pKa = 11.31GNGTIYY410 pKa = 11.07YY411 pKa = 9.44NIDD414 pKa = 3.35YY415 pKa = 10.12EE416 pKa = 4.24PDD418 pKa = 3.17KK419 pKa = 10.95MEE421 pKa = 3.9VGTYY425 pKa = 10.46VLVIAKK431 pKa = 9.98DD432 pKa = 3.44AGSVSNPYY440 pKa = 8.15VTAVCSVISSPSSEE454 pKa = 4.55FINN457 pKa = 4.03

Molecular weight: 49.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G4Q3X9|A0A1G4Q3X9_9FIRM Uncharacterized protein OS=Ruminococcaceae bacterium YRB3002 OX=1520819 GN=SAMN02910456_00854 PE=4 SV=1
MM1 pKa = 7.76FITMPFCRR9 pKa = 11.84NAIGNKK15 pKa = 7.12IWRR18 pKa = 11.84TKK20 pKa = 9.0AMKK23 pKa = 8.36MTFQPKK29 pKa = 8.95KK30 pKa = 7.58RR31 pKa = 11.84QRR33 pKa = 11.84SKK35 pKa = 9.17VHH37 pKa = 5.88GFRR40 pKa = 11.84ARR42 pKa = 11.84MRR44 pKa = 11.84TAGGRR49 pKa = 11.84KK50 pKa = 8.31VLARR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84LKK58 pKa = 10.68GRR60 pKa = 11.84KK61 pKa = 8.39QLAAA65 pKa = 4.14

Molecular weight: 7.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2644

0

2644

907111

10

2697

343.1

38.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.518 ± 0.038

1.567 ± 0.019

6.752 ± 0.042

6.664 ± 0.05

4.201 ± 0.032

7.263 ± 0.045

1.594 ± 0.015

7.608 ± 0.037

5.694 ± 0.046

8.3 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.02 ± 0.024

4.33 ± 0.029

3.517 ± 0.028

2.375 ± 0.022

4.769 ± 0.035

6.57 ± 0.036

5.71 ± 0.047

7.25 ± 0.038

0.922 ± 0.017

4.377 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski