Enterococcus phage EF-P10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Saphexavirus; unclassified Saphexavirus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S6L103|A0A1S6L103_9CAUD DNA polymerase I OS=Enterococcus phage EF-P10 OX=1958912 GN=EFP10_19 PE=3 SV=1
MM1 pKa = 7.94IEE3 pKa = 4.22LFKK6 pKa = 11.54DD7 pKa = 3.03NLTFDD12 pKa = 3.83EE13 pKa = 5.62MSDD16 pKa = 2.95IWYY19 pKa = 9.05GWDD22 pKa = 4.22NEE24 pKa = 3.9GLLYY28 pKa = 10.6GWEE31 pKa = 3.94NGEE34 pKa = 4.07AGEE37 pKa = 4.79AYY39 pKa = 10.4ALLYY43 pKa = 10.03EE44 pKa = 4.79KK45 pKa = 10.14GYY47 pKa = 11.44NMDD50 pKa = 4.11SIKK53 pKa = 10.61VLHH56 pKa = 6.42EE57 pKa = 4.26AFDD60 pKa = 4.49TIGEE64 pKa = 4.39LIGAII69 pKa = 3.92

Molecular weight: 7.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S6L0Z7|A0A1S6L0Z7_9CAUD ATP-dependent metalloprotease OS=Enterococcus phage EF-P10 OX=1958912 GN=EFP10_14 PE=4 SV=1
MM1 pKa = 7.52ANSLTPHH8 pKa = 6.28KK9 pKa = 10.27SYY11 pKa = 11.44YY12 pKa = 9.48EE13 pKa = 4.01RR14 pKa = 11.84LVEE17 pKa = 4.14ADD19 pKa = 3.22KK20 pKa = 11.81SNDD23 pKa = 2.84FRR25 pKa = 11.84KK26 pKa = 10.0ILIRR30 pKa = 11.84LMNRR34 pKa = 11.84VIMKK38 pKa = 8.7VKK40 pKa = 10.1VPIEE44 pKa = 3.93VRR46 pKa = 11.84EE47 pKa = 4.07EE48 pKa = 3.76FCMDD52 pKa = 3.9VLTMYY57 pKa = 10.31YY58 pKa = 10.51EE59 pKa = 4.47RR60 pKa = 11.84FWCSRR65 pKa = 11.84RR66 pKa = 11.84SRR68 pKa = 11.84QPSKK72 pKa = 11.0HH73 pKa = 6.25PLNILSTRR81 pKa = 11.84RR82 pKa = 11.84HH83 pKa = 4.97

Molecular weight: 10.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

127

0

127

16358

37

635

128.8

14.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.146 ± 0.295

0.746 ± 0.075

5.887 ± 0.212

8.931 ± 0.365

4.194 ± 0.173

6.156 ± 0.259

1.565 ± 0.112

6.242 ± 0.206

7.745 ± 0.267

8.748 ± 0.311

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.142

5.111 ± 0.183

3.002 ± 0.162

3.43 ± 0.201

4.457 ± 0.163

5.96 ± 0.181

5.765 ± 0.287

6.517 ± 0.288

1.351 ± 0.094

4.279 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski