Telmatospirillum siberiense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Telmatospirillum

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5353 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N3PM31|A0A2N3PM31_9PROT Uncharacterized protein OS=Telmatospirillum siberiense OX=382514 GN=CWS72_26640 PE=4 SV=1
MM1 pKa = 6.58QQASIDD7 pKa = 4.22AIIAAAKK14 pKa = 10.59ASDD17 pKa = 4.55LDD19 pKa = 4.46LNDD22 pKa = 3.49TALLLSIIRR31 pKa = 11.84TEE33 pKa = 4.39SGFNPNAAAGSSSASGLGQFINGTAAQYY61 pKa = 11.76GLTASNRR68 pKa = 11.84WSIAAQAQAAVDD80 pKa = 4.26LFIDD84 pKa = 4.24CQEE87 pKa = 4.07KK88 pKa = 10.95AGDD91 pKa = 3.68NGQDD95 pKa = 3.07SSHH98 pKa = 7.14IYY100 pKa = 10.31AYY102 pKa = 9.24WHH104 pKa = 7.41DD105 pKa = 3.85GLGTDD110 pKa = 5.35GYY112 pKa = 11.49GAGLKK117 pKa = 9.21TSNASVMPNVSAFTQALVAGNYY139 pKa = 9.56GLASSSTPISPDD151 pKa = 3.1TLTTNVDD158 pKa = 3.03GTTTLTRR165 pKa = 11.84VSNGSTYY172 pKa = 10.94SEE174 pKa = 4.26TFSAGGAANLSVTTNGQVTSSDD196 pKa = 3.52YY197 pKa = 11.16DD198 pKa = 3.47SSGRR202 pKa = 11.84LVSSATSGTSDD213 pKa = 3.32SFTAGGNTIAISGAQSLLSQGAGGYY238 pKa = 10.12SLTDD242 pKa = 3.29ASGNIYY248 pKa = 8.8TLSQLNDD255 pKa = 3.04GSGQYY260 pKa = 10.9RR261 pKa = 11.84LTSSSGLINVTLDD274 pKa = 3.23PSIVTDD280 pKa = 3.43IQLGANLKK288 pKa = 9.93IDD290 pKa = 3.52TALGDD295 pKa = 3.71FVTINTQFGSGAFGFVGSSQTAFNSPSLINIDD327 pKa = 3.21QNYY330 pKa = 8.26FQLGTPNGGLGATYY344 pKa = 9.71DD345 pKa = 3.59IQPNGSLGATIAGFLGLSGSTDD367 pKa = 3.58FASLLNEE374 pKa = 4.39TSALLPSLTAAVPTDD389 pKa = 3.63APVSVPTFSKK399 pKa = 9.12TALGVTVTDD408 pKa = 4.79LATAAQTAAGSGGDD422 pKa = 3.44GTAYY426 pKa = 10.79SITNAIWDD434 pKa = 4.17TASSLITEE442 pKa = 5.21AGQALTDD449 pKa = 3.78VADD452 pKa = 4.03VSASEE457 pKa = 4.16IANALWRR464 pKa = 11.84PYY466 pKa = 8.97YY467 pKa = 10.07QSLMSYY473 pKa = 10.6FNDD476 pKa = 3.1VPAATGVLNTDD487 pKa = 3.4AGSVLLPGGSGVTVGDD503 pKa = 4.04LVAAAVDD510 pKa = 4.36SNPTPSFTDD519 pKa = 3.63PLLLDD524 pKa = 3.85LTGGGIGVSSWIRR537 pKa = 11.84SPVYY541 pKa = 10.29FDD543 pKa = 4.01TNVKK547 pKa = 10.42ADD549 pKa = 3.65ANGNPTTTPDD559 pKa = 3.43GMEE562 pKa = 5.11HH563 pKa = 5.03EE564 pKa = 4.65TAWMKK569 pKa = 10.92AGTGMLVFEE578 pKa = 4.85SGGVVAPITNITQTVSEE595 pKa = 4.64FLNAGATPGKK605 pKa = 10.37YY606 pKa = 10.04ADD608 pKa = 4.3GLAALL613 pKa = 4.73

Molecular weight: 61.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N3PRL2|A0A2N3PRL2_9PROT Uncharacterized protein OS=Telmatospirillum siberiense OX=382514 GN=CWS72_18540 PE=4 SV=1
MM1 pKa = 7.3TSAALPARR9 pKa = 11.84AGSRR13 pKa = 11.84TLQSIARR20 pKa = 11.84IGSAATLLILFAGIGSTVGWPGLRR44 pKa = 11.84FDD46 pKa = 5.49FLSQHH51 pKa = 6.4SGFQISEE58 pKa = 4.18SLFQIGADD66 pKa = 3.52APIWLMLIAGLINTIRR82 pKa = 11.84VSAMAMVLTIPLGIALALLRR102 pKa = 11.84GSAFRR107 pKa = 11.84PGRR110 pKa = 11.84WLATGVIEE118 pKa = 5.4PIRR121 pKa = 11.84NTPVLLQLFLWYY133 pKa = 10.53GIVTQLLPGPRR144 pKa = 11.84QAVEE148 pKa = 4.04IPPGILLCNRR158 pKa = 11.84GLYY161 pKa = 9.74FPWFDD166 pKa = 3.29GSGVTWPKK174 pKa = 10.35LAGFNIEE181 pKa = 4.4GGLSISPEE189 pKa = 3.77FTVLVFGLALFHH201 pKa = 6.45GCYY204 pKa = 9.56LAEE207 pKa = 4.13IFRR210 pKa = 11.84AGFLAVPRR218 pKa = 11.84GQRR221 pKa = 11.84DD222 pKa = 3.21AAKK225 pKa = 9.56ALSMPAFSTLRR236 pKa = 11.84KK237 pKa = 9.37ILLPQALAFALPSTAAQILALVKK260 pKa = 10.73NSSLAIAIGFADD272 pKa = 5.32FVAVLNTSVNQNGRR286 pKa = 11.84TLEE289 pKa = 3.93GLIVIILVFLGLNTLLGAAVEE310 pKa = 4.22KK311 pKa = 9.67GTRR314 pKa = 11.84RR315 pKa = 11.84FGKK318 pKa = 8.75ATRR321 pKa = 11.84GQLAVEE327 pKa = 4.19RR328 pKa = 11.84QSMEE332 pKa = 3.72DD333 pKa = 3.18RR334 pKa = 11.84KK335 pKa = 10.01PAGLRR340 pKa = 11.84VCSDD344 pKa = 2.6IAMIATATAIFLPAIVRR361 pKa = 11.84IFRR364 pKa = 11.84WGVLDD369 pKa = 4.05AAWAGSPADD378 pKa = 3.34CAMANGACWAVVTEE392 pKa = 4.45KK393 pKa = 11.17APLLLFGPFPGAEE406 pKa = 3.72TWRR409 pKa = 11.84PLIATLLIILFIIALGIDD427 pKa = 3.87RR428 pKa = 11.84LRR430 pKa = 11.84HH431 pKa = 5.65AGVSLAALAATMIGWIWLLGGGAGLASVPTNAWGGLSLTSGLAVAVVMGACLLALPLALARR492 pKa = 11.84RR493 pKa = 11.84SPHH496 pKa = 5.18KK497 pKa = 9.18TIHH500 pKa = 5.67IPAVIFIEE508 pKa = 4.16IFRR511 pKa = 11.84GVPLVALLLSADD523 pKa = 3.35ILIPGLLPSGWQFDD537 pKa = 4.34KK538 pKa = 11.04LWRR541 pKa = 11.84AYY543 pKa = 10.28AAITLLASVNLAEE556 pKa = 4.44VMRR559 pKa = 11.84GALAAVAPGQTEE571 pKa = 3.8AARR574 pKa = 11.84ALGLSARR581 pKa = 11.84RR582 pKa = 11.84SFLLVILPQAIRR594 pKa = 11.84IAMPALVNVFVGAIKK609 pKa = 9.5DD610 pKa = 3.61TSLVLIVGILDD621 pKa = 3.1ITGAAKK627 pKa = 10.3AAVADD632 pKa = 3.97PAWRR636 pKa = 11.84NFAPEE641 pKa = 3.89IYY643 pKa = 10.28LFLAAVYY650 pKa = 8.78FALCFPIGRR659 pKa = 11.84LARR662 pKa = 11.84RR663 pKa = 11.84MTDD666 pKa = 2.72QTTADD671 pKa = 3.48SPSRR675 pKa = 11.84KK676 pKa = 9.18RR677 pKa = 11.84SISAASLL684 pKa = 3.4

Molecular weight: 72.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5353

0

5353

1820509

26

7446

340.1

36.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.178 ± 0.051

0.951 ± 0.013

5.6 ± 0.026

5.362 ± 0.033

3.626 ± 0.025

8.73 ± 0.042

2.085 ± 0.017

5.189 ± 0.025

3.102 ± 0.025

10.695 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.336 ± 0.017

2.586 ± 0.022

5.119 ± 0.031

3.178 ± 0.022

7.019 ± 0.049

5.841 ± 0.041

5.425 ± 0.058

7.507 ± 0.029

1.309 ± 0.016

2.16 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski