Proteome-pI 2.0 - Proteome Isoelectric Point Database

Juncus maritimus associated virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; unclassified Geminiviridae

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A2I8B2J7|A0A2I8B2J7_9GEMI Replication enhancer OS=Juncus maritimus associated virus OX=2093273 PE=3 SV=1

MM1 pKa = 7.79ADD3 pKa = 3.66KK4 pKa = 10.65FCNYY8 pKa = 9.74LDD10 pKa = 3.62KK11 pKa = 11.17RR12 pKa = 11.84QVEE15 pKa = 4.22PAEE18 pKa = 4.28LLPLITRR25 pKa = 11.84WIVEE29 pKa = 4.33KK30 pKa = 11.01GSAQQFIDD38 pKa = 4.66WINGLQRR45 pKa = 11.84EE46 pKa = 4.28EE47 pKa = 4.49KK48 pKa = 10.53EE49 pKa = 3.7IFRR52 pKa = 11.84ALNACSDD59 pKa = 3.92SKK61 pKa = 11.52EE62 pKa = 4.59DD63 pKa = 4.48DD64 pKa = 4.19DD65 pKa = 6.56DD66 pKa = 5.29PEE68 pKa = 4.24EE69 pKa = 4.05VQLRR73 pKa = 11.84VGPSSSVPKK82 pKa = 10.22PILQAQSDD90 pKa = 4.26GLQEE94 pKa = 3.91EE95 pKa = 4.76VQIHH99 pKa = 6.15ISS101 pKa = 3.18

Molecular weight: 11.52 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A2I8B2J9|A0A2I8B2J9_9GEMI Capsid protein OS=Juncus maritimus associated virus OX=2093273 PE=4 SV=1

MM1 pKa = 8.3DD2 pKa = 4.78SRR4 pKa = 11.84TGDD7 pKa = 3.64TITAHH12 pKa = 5.71QANSGIWFDD21 pKa = 3.85EE22 pKa = 4.19LKK24 pKa = 11.2NPINVNIDD32 pKa = 3.39KK33 pKa = 10.69DD34 pKa = 3.37WDD36 pKa = 3.42IFPTFNKK43 pKa = 9.68GFQVTVKK50 pKa = 10.3FNHH53 pKa = 6.19NLRR56 pKa = 11.84KK57 pKa = 9.79ALGLHH62 pKa = 5.97KK63 pKa = 10.44CWITFKK69 pKa = 10.31IWTTLKK75 pKa = 10.95SFGKK79 pKa = 10.27KK80 pKa = 9.48SLEE83 pKa = 3.64HH84 pKa = 7.18RR85 pKa = 11.84IRR87 pKa = 11.84FHH89 pKa = 7.71LMSFLDD95 pKa = 3.62SCGVISINLMIRR107 pKa = 11.84AINKK111 pKa = 9.58LIAKK115 pKa = 7.43CLWIVNIEE123 pKa = 4.02EE124 pKa = 3.73QRR126 pKa = 11.84NIVMFNIYY134 pKa = 10.27

Molecular weight: 15.73 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
5	0	5	984	101	354	196.8	22.55

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
5.589 ± 1.059	1.931 ± 0.572	5.386 ± 0.393	4.878 ± 1.041	4.675 ± 0.672	5.894 ± 0.585	3.354 ± 0.567	7.52 ± 1.085	6.402 ± 0.563	6.504 ± 0.755

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
1.931 ± 0.519	6.402 ± 0.765	5.691 ± 1.104	5.386 ± 1.537	5.793 ± 0.969	8.74 ± 0.965	3.963 ± 0.448	3.963 ± 0.378	1.931 ± 0.387	4.065 ± 1.174

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski