Amino acid dipepetide frequency for Pseudomonas phage PspYZU08

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.355AlaAla: 11.355 ± 1.143
0.811AlaCys: 0.811 ± 0.263
5.678AlaAsp: 5.678 ± 0.772
5.434AlaGlu: 5.434 ± 0.6
3.488AlaPhe: 3.488 ± 0.542
7.949AlaGly: 7.949 ± 0.9
1.217AlaHis: 1.217 ± 0.27
5.759AlaIle: 5.759 ± 0.597
6.894AlaLys: 6.894 ± 0.749
8.192AlaLeu: 8.192 ± 0.824
2.514AlaMet: 2.514 ± 0.456
3.893AlaAsn: 3.893 ± 0.672
3.163AlaPro: 3.163 ± 0.515
5.515AlaGln: 5.515 ± 0.637
5.84AlaArg: 5.84 ± 0.876
5.11AlaSer: 5.11 ± 0.895
5.434AlaThr: 5.434 ± 0.756
5.353AlaVal: 5.353 ± 0.84
1.703AlaTrp: 1.703 ± 0.481
3.163AlaTyr: 3.163 ± 0.62
0.0AlaXaa: 0.0 ± 0.0
Cys
0.811CysAla: 0.811 ± 0.259
0.081CysCys: 0.081 ± 0.081
0.73CysAsp: 0.73 ± 0.332
0.487CysGlu: 0.487 ± 0.233
0.406CysPhe: 0.406 ± 0.196
0.406CysGly: 0.406 ± 0.149
0.243CysHis: 0.243 ± 0.139
0.649CysIle: 0.649 ± 0.233
0.406CysLys: 0.406 ± 0.22
0.568CysLeu: 0.568 ± 0.245
0.243CysMet: 0.243 ± 0.125
0.324CysAsn: 0.324 ± 0.166
0.568CysPro: 0.568 ± 0.253
0.324CysGln: 0.324 ± 0.156
0.811CysArg: 0.811 ± 0.346
0.487CysSer: 0.487 ± 0.189
0.162CysThr: 0.162 ± 0.124
0.649CysVal: 0.649 ± 0.227
0.0CysTrp: 0.0 ± 0.0
0.081CysTyr: 0.081 ± 0.077
0.0CysXaa: 0.0 ± 0.0
Asp
6.57AspAla: 6.57 ± 0.59
0.568AspCys: 0.568 ± 0.217
4.704AspAsp: 4.704 ± 0.639
4.785AspGlu: 4.785 ± 0.855
2.433AspPhe: 2.433 ± 0.448
6.408AspGly: 6.408 ± 0.862
1.136AspHis: 1.136 ± 0.315
3.407AspIle: 3.407 ± 0.562
3.407AspLys: 3.407 ± 0.497
4.623AspLeu: 4.623 ± 0.621
1.866AspMet: 1.866 ± 0.262
1.703AspAsn: 1.703 ± 0.244
2.758AspPro: 2.758 ± 0.596
3.244AspGln: 3.244 ± 0.431
2.758AspArg: 2.758 ± 0.554
2.352AspSer: 2.352 ± 0.459
3.569AspThr: 3.569 ± 0.474
4.055AspVal: 4.055 ± 0.669
0.892AspTrp: 0.892 ± 0.301
2.028AspTyr: 2.028 ± 0.37
0.0AspXaa: 0.0 ± 0.0
Glu
8.03GluAla: 8.03 ± 0.726
0.487GluCys: 0.487 ± 0.208
3.001GluAsp: 3.001 ± 0.373
5.272GluGlu: 5.272 ± 1.04
3.082GluPhe: 3.082 ± 0.519
4.623GluGly: 4.623 ± 0.578
1.541GluHis: 1.541 ± 0.392
3.407GluIle: 3.407 ± 0.568
2.514GluLys: 2.514 ± 0.36
5.11GluLeu: 5.11 ± 0.707
2.109GluMet: 2.109 ± 0.463
2.596GluAsn: 2.596 ± 0.465
2.19GluPro: 2.19 ± 0.524
3.893GluGln: 3.893 ± 0.795
4.542GluArg: 4.542 ± 0.446
3.082GluSer: 3.082 ± 0.581
4.542GluThr: 4.542 ± 0.594
4.948GluVal: 4.948 ± 0.586
1.541GluTrp: 1.541 ± 0.386
2.271GluTyr: 2.271 ± 0.378
0.0GluXaa: 0.0 ± 0.0
Phe
2.92PheAla: 2.92 ± 0.469
0.406PheCys: 0.406 ± 0.21
2.514PheAsp: 2.514 ± 0.366
2.596PheGlu: 2.596 ± 0.509
1.622PhePhe: 1.622 ± 0.363
3.163PheGly: 3.163 ± 0.546
0.568PheHis: 0.568 ± 0.203
1.136PheIle: 1.136 ± 0.423
2.514PheLys: 2.514 ± 0.551
3.893PheLeu: 3.893 ± 0.499
0.973PheMet: 0.973 ± 0.295
1.784PheAsn: 1.784 ± 0.41
1.703PhePro: 1.703 ± 0.337
1.541PheGln: 1.541 ± 0.378
1.784PheArg: 1.784 ± 0.36
2.109PheSer: 2.109 ± 0.336
2.677PheThr: 2.677 ± 0.485
2.677PheVal: 2.677 ± 0.447
0.487PheTrp: 0.487 ± 0.236
1.136PheTyr: 1.136 ± 0.309
0.0PheXaa: 0.0 ± 0.0
Gly
6.651GlyAla: 6.651 ± 0.766
0.973GlyCys: 0.973 ± 0.28
6.083GlyAsp: 6.083 ± 0.566
4.867GlyGlu: 4.867 ± 0.643
3.244GlyPhe: 3.244 ± 0.48
6.651GlyGly: 6.651 ± 0.645
1.703GlyHis: 1.703 ± 0.349
4.704GlyIle: 4.704 ± 0.611
5.272GlyLys: 5.272 ± 0.749
6.894GlyLeu: 6.894 ± 0.655
2.109GlyMet: 2.109 ± 0.337
2.514GlyAsn: 2.514 ± 0.492
2.271GlyPro: 2.271 ± 0.405
3.812GlyGln: 3.812 ± 0.672
4.867GlyArg: 4.867 ± 0.573
5.191GlySer: 5.191 ± 0.666
3.974GlyThr: 3.974 ± 0.622
4.542GlyVal: 4.542 ± 0.511
1.46GlyTrp: 1.46 ± 0.35
2.839GlyTyr: 2.839 ± 0.478
0.0GlyXaa: 0.0 ± 0.0
His
1.46HisAla: 1.46 ± 0.311
0.243HisCys: 0.243 ± 0.134
1.379HisAsp: 1.379 ± 0.395
1.541HisGlu: 1.541 ± 0.411
0.649HisPhe: 0.649 ± 0.325
2.19HisGly: 2.19 ± 0.316
0.406HisHis: 0.406 ± 0.212
1.217HisIle: 1.217 ± 0.363
0.892HisLys: 0.892 ± 0.273
1.947HisLeu: 1.947 ± 0.521
0.811HisMet: 0.811 ± 0.265
0.649HisAsn: 0.649 ± 0.185
0.568HisPro: 0.568 ± 0.192
0.324HisGln: 0.324 ± 0.161
1.136HisArg: 1.136 ± 0.325
1.136HisSer: 1.136 ± 0.349
0.892HisThr: 0.892 ± 0.295
1.054HisVal: 1.054 ± 0.248
0.568HisTrp: 0.568 ± 0.234
0.811HisTyr: 0.811 ± 0.229
0.0HisXaa: 0.0 ± 0.0
Ile
5.029IleAla: 5.029 ± 0.598
0.73IleCys: 0.73 ± 0.292
3.488IleAsp: 3.488 ± 0.39
3.488IleGlu: 3.488 ± 0.538
1.622IlePhe: 1.622 ± 0.351
3.569IleGly: 3.569 ± 0.493
1.054IleHis: 1.054 ± 0.28
2.028IleIle: 2.028 ± 0.423
3.407IleLys: 3.407 ± 0.595
3.812IleLeu: 3.812 ± 0.535
0.973IleMet: 0.973 ± 0.263
1.379IleAsn: 1.379 ± 0.33
2.596IlePro: 2.596 ± 0.456
3.001IleGln: 3.001 ± 0.371
3.65IleArg: 3.65 ± 0.592
2.758IleSer: 2.758 ± 0.524
2.433IleThr: 2.433 ± 0.437
3.569IleVal: 3.569 ± 0.507
0.324IleTrp: 0.324 ± 0.157
1.622IleTyr: 1.622 ± 0.394
0.0IleXaa: 0.0 ± 0.0
Lys
7.138LysAla: 7.138 ± 0.772
0.406LysCys: 0.406 ± 0.203
3.974LysAsp: 3.974 ± 0.479
5.434LysGlu: 5.434 ± 0.607
2.271LysPhe: 2.271 ± 0.409
5.272LysGly: 5.272 ± 0.736
1.703LysHis: 1.703 ± 0.315
2.352LysIle: 2.352 ± 0.332
3.163LysLys: 3.163 ± 0.59
4.704LysLeu: 4.704 ± 0.547
0.973LysMet: 0.973 ± 0.32
2.19LysAsn: 2.19 ± 0.448
2.271LysPro: 2.271 ± 0.56
3.244LysGln: 3.244 ± 0.585
3.488LysArg: 3.488 ± 0.498
2.677LysSer: 2.677 ± 0.358
3.244LysThr: 3.244 ± 0.639
4.218LysVal: 4.218 ± 0.627
0.649LysTrp: 0.649 ± 0.269
1.784LysTyr: 1.784 ± 0.42
0.0LysXaa: 0.0 ± 0.0
Leu
7.624LeuAla: 7.624 ± 0.74
0.568LeuCys: 0.568 ± 0.247
4.461LeuAsp: 4.461 ± 0.481
5.353LeuGlu: 5.353 ± 0.753
2.028LeuPhe: 2.028 ± 0.398
4.461LeuGly: 4.461 ± 0.564
1.46LeuHis: 1.46 ± 0.272
5.029LeuIle: 5.029 ± 0.548
5.678LeuLys: 5.678 ± 0.675
6.002LeuLeu: 6.002 ± 0.858
2.109LeuMet: 2.109 ± 0.452
4.785LeuAsn: 4.785 ± 0.592
2.596LeuPro: 2.596 ± 0.376
4.055LeuGln: 4.055 ± 0.502
5.11LeuArg: 5.11 ± 0.406
5.759LeuSer: 5.759 ± 0.414
3.893LeuThr: 3.893 ± 0.675
5.515LeuVal: 5.515 ± 0.779
0.892LeuTrp: 0.892 ± 0.291
2.514LeuTyr: 2.514 ± 0.402
0.0LeuXaa: 0.0 ± 0.0
Met
3.325MetAla: 3.325 ± 0.393
0.162MetCys: 0.162 ± 0.116
1.784MetAsp: 1.784 ± 0.345
1.703MetGlu: 1.703 ± 0.359
0.73MetPhe: 0.73 ± 0.326
2.758MetGly: 2.758 ± 0.402
0.568MetHis: 0.568 ± 0.303
1.298MetIle: 1.298 ± 0.267
1.947MetLys: 1.947 ± 0.369
2.433MetLeu: 2.433 ± 0.448
0.649MetMet: 0.649 ± 0.258
0.811MetAsn: 0.811 ± 0.235
1.298MetPro: 1.298 ± 0.312
1.217MetGln: 1.217 ± 0.346
1.054MetArg: 1.054 ± 0.266
2.028MetSer: 2.028 ± 0.339
2.028MetThr: 2.028 ± 0.343
1.784MetVal: 1.784 ± 0.373
0.487MetTrp: 0.487 ± 0.16
0.811MetTyr: 0.811 ± 0.283
0.0MetXaa: 0.0 ± 0.0
Asn
3.325AsnAla: 3.325 ± 0.521
0.162AsnCys: 0.162 ± 0.094
2.109AsnAsp: 2.109 ± 0.42
2.596AsnGlu: 2.596 ± 0.453
2.109AsnPhe: 2.109 ± 0.352
3.488AsnGly: 3.488 ± 0.385
0.568AsnHis: 0.568 ± 0.254
1.622AsnIle: 1.622 ± 0.31
2.271AsnLys: 2.271 ± 0.441
3.244AsnLeu: 3.244 ± 0.483
0.892AsnMet: 0.892 ± 0.21
1.217AsnAsn: 1.217 ± 0.276
2.92AsnPro: 2.92 ± 0.398
2.109AsnGln: 2.109 ± 0.323
1.866AsnArg: 1.866 ± 0.391
1.947AsnSer: 1.947 ± 0.44
1.136AsnThr: 1.136 ± 0.322
2.839AsnVal: 2.839 ± 0.501
0.487AsnTrp: 0.487 ± 0.183
1.298AsnTyr: 1.298 ± 0.358
0.0AsnXaa: 0.0 ± 0.0
Pro
3.325ProAla: 3.325 ± 0.422
0.243ProCys: 0.243 ± 0.124
3.325ProAsp: 3.325 ± 0.639
3.407ProGlu: 3.407 ± 0.539
1.622ProPhe: 1.622 ± 0.315
2.352ProGly: 2.352 ± 0.487
0.892ProHis: 0.892 ± 0.222
1.217ProIle: 1.217 ± 0.331
2.028ProLys: 2.028 ± 0.467
2.514ProLeu: 2.514 ± 0.452
1.136ProMet: 1.136 ± 0.291
2.028ProAsn: 2.028 ± 0.371
1.217ProPro: 1.217 ± 0.407
2.028ProGln: 2.028 ± 0.449
1.947ProArg: 1.947 ± 0.396
2.19ProSer: 2.19 ± 0.564
1.379ProThr: 1.379 ± 0.27
2.839ProVal: 2.839 ± 0.513
0.487ProTrp: 0.487 ± 0.176
1.379ProTyr: 1.379 ± 0.294
0.0ProXaa: 0.0 ± 0.0
Gln
6.732GlnAla: 6.732 ± 0.802
0.568GlnCys: 0.568 ± 0.215
2.514GlnAsp: 2.514 ± 0.488
3.325GlnGlu: 3.325 ± 0.471
2.028GlnPhe: 2.028 ± 0.352
4.055GlnGly: 4.055 ± 0.712
0.892GlnHis: 0.892 ± 0.325
2.596GlnIle: 2.596 ± 0.369
2.839GlnLys: 2.839 ± 0.422
4.218GlnLeu: 4.218 ± 0.652
1.46GlnMet: 1.46 ± 0.35
1.136GlnAsn: 1.136 ± 0.213
1.866GlnPro: 1.866 ± 0.364
2.433GlnGln: 2.433 ± 0.553
3.244GlnArg: 3.244 ± 0.468
2.758GlnSer: 2.758 ± 0.457
1.136GlnThr: 1.136 ± 0.316
3.163GlnVal: 3.163 ± 0.592
0.811GlnTrp: 0.811 ± 0.267
1.541GlnTyr: 1.541 ± 0.295
0.0GlnXaa: 0.0 ± 0.0
Arg
4.461ArgAla: 4.461 ± 0.498
0.406ArgCys: 0.406 ± 0.17
3.488ArgAsp: 3.488 ± 0.634
3.812ArgGlu: 3.812 ± 0.591
2.19ArgPhe: 2.19 ± 0.391
4.461ArgGly: 4.461 ± 0.524
1.298ArgHis: 1.298 ± 0.342
3.407ArgIle: 3.407 ± 0.417
4.055ArgLys: 4.055 ± 0.55
4.461ArgLeu: 4.461 ± 0.554
1.703ArgMet: 1.703 ± 0.506
2.514ArgAsn: 2.514 ± 0.422
1.784ArgPro: 1.784 ± 0.398
3.488ArgGln: 3.488 ± 0.595
2.433ArgArg: 2.433 ± 0.37
4.542ArgSer: 4.542 ± 0.781
3.082ArgThr: 3.082 ± 0.487
2.758ArgVal: 2.758 ± 0.439
0.568ArgTrp: 0.568 ± 0.315
1.703ArgTyr: 1.703 ± 0.45
0.0ArgXaa: 0.0 ± 0.0
Ser
6.408SerAla: 6.408 ± 0.949
0.324SerCys: 0.324 ± 0.165
4.218SerAsp: 4.218 ± 0.508
3.163SerGlu: 3.163 ± 0.469
2.839SerPhe: 2.839 ± 0.442
5.759SerGly: 5.759 ± 0.73
1.298SerHis: 1.298 ± 0.273
2.839SerIle: 2.839 ± 0.556
3.163SerLys: 3.163 ± 0.513
3.407SerLeu: 3.407 ± 0.501
1.622SerMet: 1.622 ± 0.354
2.596SerAsn: 2.596 ± 0.402
1.784SerPro: 1.784 ± 0.28
2.271SerGln: 2.271 ± 0.407
2.596SerArg: 2.596 ± 0.511
3.893SerSer: 3.893 ± 0.792
2.271SerThr: 2.271 ± 0.401
2.839SerVal: 2.839 ± 0.415
1.054SerTrp: 1.054 ± 0.216
2.677SerTyr: 2.677 ± 0.345
0.0SerXaa: 0.0 ± 0.0
Thr
4.055ThrAla: 4.055 ± 0.535
0.243ThrCys: 0.243 ± 0.145
3.974ThrAsp: 3.974 ± 0.471
3.893ThrGlu: 3.893 ± 0.343
1.784ThrPhe: 1.784 ± 0.453
4.704ThrGly: 4.704 ± 0.745
1.136ThrHis: 1.136 ± 0.263
2.758ThrIle: 2.758 ± 0.359
4.137ThrLys: 4.137 ± 0.526
4.785ThrLeu: 4.785 ± 0.527
1.622ThrMet: 1.622 ± 0.346
1.541ThrAsn: 1.541 ± 0.369
2.109ThrPro: 2.109 ± 0.363
2.109ThrGln: 2.109 ± 0.372
2.19ThrArg: 2.19 ± 0.441
3.244ThrSer: 3.244 ± 0.637
2.839ThrThr: 2.839 ± 0.466
3.325ThrVal: 3.325 ± 0.548
0.73ThrTrp: 0.73 ± 0.263
1.46ThrTyr: 1.46 ± 0.341
0.0ThrXaa: 0.0 ± 0.0
Val
5.191ValAla: 5.191 ± 0.878
0.487ValCys: 0.487 ± 0.209
3.001ValAsp: 3.001 ± 0.437
4.948ValGlu: 4.948 ± 0.579
2.352ValPhe: 2.352 ± 0.516
4.137ValGly: 4.137 ± 0.653
1.136ValHis: 1.136 ± 0.348
3.163ValIle: 3.163 ± 0.616
3.65ValLys: 3.65 ± 0.464
5.029ValLeu: 5.029 ± 0.734
2.92ValMet: 2.92 ± 0.479
2.677ValAsn: 2.677 ± 0.443
2.271ValPro: 2.271 ± 0.399
2.352ValGln: 2.352 ± 0.283
3.893ValArg: 3.893 ± 0.502
3.488ValSer: 3.488 ± 0.637
4.299ValThr: 4.299 ± 0.48
4.948ValVal: 4.948 ± 0.71
0.892ValTrp: 0.892 ± 0.288
2.514ValTyr: 2.514 ± 0.494
0.0ValXaa: 0.0 ± 0.0
Trp
1.622TrpAla: 1.622 ± 0.45
0.162TrpCys: 0.162 ± 0.106
0.568TrpAsp: 0.568 ± 0.198
0.649TrpGlu: 0.649 ± 0.193
0.568TrpPhe: 0.568 ± 0.251
0.649TrpGly: 0.649 ± 0.212
0.406TrpHis: 0.406 ± 0.206
0.324TrpIle: 0.324 ± 0.185
1.298TrpLys: 1.298 ± 0.315
1.541TrpLeu: 1.541 ± 0.346
0.649TrpMet: 0.649 ± 0.225
0.487TrpAsn: 0.487 ± 0.208
0.324TrpPro: 0.324 ± 0.162
0.892TrpGln: 0.892 ± 0.226
1.136TrpArg: 1.136 ± 0.325
0.568TrpSer: 0.568 ± 0.292
1.136TrpThr: 1.136 ± 0.306
0.973TrpVal: 0.973 ± 0.279
0.0TrpTrp: 0.0 ± 0.0
0.406TrpTyr: 0.406 ± 0.177
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.433TyrAla: 2.433 ± 0.511
0.406TyrCys: 0.406 ± 0.196
2.271TyrAsp: 2.271 ± 0.389
2.109TyrGlu: 2.109 ± 0.362
1.136TyrPhe: 1.136 ± 0.307
3.569TyrGly: 3.569 ± 0.436
0.649TyrHis: 0.649 ± 0.247
1.622TyrIle: 1.622 ± 0.382
1.622TyrLys: 1.622 ± 0.349
2.514TyrLeu: 2.514 ± 0.301
1.46TyrMet: 1.46 ± 0.306
1.379TyrAsn: 1.379 ± 0.312
1.298TyrPro: 1.298 ± 0.36
1.46TyrGln: 1.46 ± 0.332
2.271TyrArg: 2.271 ± 0.387
1.703TyrSer: 1.703 ± 0.454
2.514TyrThr: 2.514 ± 0.391
1.298TyrVal: 1.298 ± 0.31
0.324TyrTrp: 0.324 ± 0.14
0.811TyrTyr: 0.811 ± 0.193
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (12330 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski