Streptococcus phage Javan465

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ADI0|A0A4D6ADI0_9CAUD Uncharacterized protein OS=Streptococcus phage Javan465 OX=2548186 GN=Javan465_0046 PE=4 SV=1
MM1 pKa = 7.64NYY3 pKa = 10.44LEE5 pKa = 4.33YY6 pKa = 11.13ALAYY10 pKa = 10.27LEE12 pKa = 4.77RR13 pKa = 11.84EE14 pKa = 4.39LEE16 pKa = 4.5IIDD19 pKa = 3.82NDD21 pKa = 3.93VIEE24 pKa = 4.46VEE26 pKa = 4.47LPNGGWEE33 pKa = 4.17FAPNPYY39 pKa = 9.89YY40 pKa = 10.49EE41 pKa = 5.01EE42 pKa = 4.65GLHH45 pKa = 6.64DD46 pKa = 3.79SPYY49 pKa = 10.48YY50 pKa = 10.02RR51 pKa = 11.84SQVAKK56 pKa = 10.55DD57 pKa = 3.09ILDD60 pKa = 3.46IKK62 pKa = 10.8GLLGRR67 pKa = 4.72

Molecular weight: 7.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AHG1|A0A4D6AHG1_9CAUD Terminase small subunit OS=Streptococcus phage Javan465 OX=2548186 GN=Javan465_0025 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84GLDD5 pKa = 3.09RR6 pKa = 11.84FLRR9 pKa = 11.84SVEE12 pKa = 4.05RR13 pKa = 11.84KK14 pKa = 8.65QKK16 pKa = 9.38SVRR19 pKa = 11.84IAVDD23 pKa = 3.31KK24 pKa = 10.73EE25 pKa = 4.09LSKK28 pKa = 10.9SAARR32 pKa = 11.84IEE34 pKa = 4.15RR35 pKa = 11.84QAKK38 pKa = 9.19ILAPVDD44 pKa = 3.94TGWLRR49 pKa = 11.84AQIYY53 pKa = 10.15SEE55 pKa = 4.1QQRR58 pKa = 11.84LLHH61 pKa = 5.57YY62 pKa = 9.97RR63 pKa = 11.84VVSPALYY70 pKa = 10.27SIYY73 pKa = 10.79LEE75 pKa = 4.28LGTRR79 pKa = 11.84KK80 pKa = 8.85MEE82 pKa = 4.3AQSFLDD88 pKa = 3.47PALRR92 pKa = 11.84KK93 pKa = 8.48EE94 pKa = 4.46WPVLMANIKK103 pKa = 10.68KK104 pKa = 8.93MFKK107 pKa = 10.27RR108 pKa = 3.68

Molecular weight: 12.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

11786

38

1460

245.5

27.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.239 ± 0.752

0.424 ± 0.092

6.151 ± 0.499

7.314 ± 0.656

3.674 ± 0.184

6.194 ± 0.451

1.171 ± 0.144

7.127 ± 0.327

8.748 ± 0.637

8.714 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.461 ± 0.172

5.422 ± 0.249

2.825 ± 0.196

3.784 ± 0.31

4.463 ± 0.397

6.593 ± 0.803

5.931 ± 0.307

5.939 ± 0.197

1.044 ± 0.119

3.784 ± 0.479
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski