Streptococcus phage YMC-2011
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F8HGP3|F8HGP3_9CAUD Uncharacterized protein OS=Streptococcus phage YMC-2011 OX=1051631 GN=Ssal_phage00009 PE=4 SV=1
MM1 pKa = 7.31 ATDD4 pKa = 3.32 NDD6 pKa = 3.98 IIQFAEE12 pKa = 3.9 NLADD16 pKa = 4.08 AGVGTDD22 pKa = 5.53 ADD24 pKa = 4.57 GAWGTQCVDD33 pKa = 3.86 LPNSISINFFGRR45 pKa = 11.84 ALWGNAIDD53 pKa = 4.82 LLNSAAEE60 pKa = 3.8 AGYY63 pKa = 9.78 EE64 pKa = 3.97 VEE66 pKa = 4.4 YY67 pKa = 11.0 NQVGNLDD74 pKa = 3.35 SRR76 pKa = 11.84 PRR78 pKa = 11.84 RR79 pKa = 11.84 GAVFVMDD86 pKa = 3.68 TTYY89 pKa = 10.83 IAGHH93 pKa = 6.93 PYY95 pKa = 10.14 GHH97 pKa = 6.99 TGLVIEE103 pKa = 5.45 DD104 pKa = 3.4 SDD106 pKa = 4.88 GYY108 pKa = 8.63 TMRR111 pKa = 11.84 TIEE114 pKa = 4.63 QNIDD118 pKa = 2.96 GNADD122 pKa = 3.3 SLYY125 pKa = 9.74 VGGPARR131 pKa = 11.84 YY132 pKa = 7.46 NTRR135 pKa = 11.84 NFDD138 pKa = 4.25 GIVGWFYY145 pKa = 11.19 FPTDD149 pKa = 3.63 NQSQAPAPTLTPFDD163 pKa = 4.52 GIITINEE170 pKa = 3.86 EE171 pKa = 3.83 TGTFTVEE178 pKa = 3.56 VSALNVRR185 pKa = 11.84 AGAGLGAEE193 pKa = 4.03 IVAVYY198 pKa = 10.09 GAGEE202 pKa = 4.27 TINYY206 pKa = 9.23 DD207 pKa = 3.24 GWCDD211 pKa = 3.03 VDD213 pKa = 4.36 GYY215 pKa = 10.88 IWISYY220 pKa = 9.71 ISWSGNRR227 pKa = 11.84 RR228 pKa = 11.84 YY229 pKa = 10.28 VAVGQSEE236 pKa = 3.98 NGRR239 pKa = 11.84 RR240 pKa = 11.84 VTSFGSFAA248 pKa = 4.1
Molecular weight: 26.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.8
IPC2_protein 3.999
IPC_protein 3.986
Toseland 3.77
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.897
Rodwell 3.808
Grimsley 3.681
Solomon 3.961
Lehninger 3.923
Nozaki 4.088
DTASelect 4.317
Thurlkill 3.821
EMBOSS 3.91
Sillero 4.101
Patrickios 0.871
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 3.988
Protein with the highest isoelectric point:
>tr|F8HGQ2|F8HGQ2_9CAUD Phage protein OS=Streptococcus phage YMC-2011 OX=1051631 GN=Ssal_phage00020 PE=4 SV=1
MM1 pKa = 7.73 RR2 pKa = 11.84 QRR4 pKa = 11.84 CQKK7 pKa = 10.05 SKK9 pKa = 10.75 RR10 pKa = 11.84 YY11 pKa = 9.93 DD12 pKa = 3.0 ISFLRR17 pKa = 11.84 KK18 pKa = 7.97 TKK20 pKa = 9.86 KK21 pKa = 9.96 QKK23 pKa = 9.93 NACSQNEE30 pKa = 4.05 QLPKK34 pKa = 10.86 GVGG37 pKa = 3.1
Molecular weight: 4.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.262
IPC2_protein 9.677
IPC_protein 9.853
Toseland 10.804
ProMoST 10.277
Dawson 10.862
Bjellqvist 10.467
Wikipedia 10.979
Rodwell 11.433
Grimsley 10.877
Solomon 10.921
Lehninger 10.906
Nozaki 10.774
DTASelect 10.452
Thurlkill 10.774
EMBOSS 11.169
Sillero 10.789
Patrickios 11.199
IPC_peptide 10.935
IPC2_peptide 9.326
IPC2.peptide.svr19 8.588
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
12401
37
952
225.5
25.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.693 ± 0.534
0.702 ± 0.162
6.379 ± 0.25
7.04 ± 0.492
4.225 ± 0.183
6.951 ± 0.494
1.387 ± 0.137
6.298 ± 0.223
8.378 ± 0.406
7.564 ± 0.285
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.33 ± 0.198
5.79 ± 0.254
3.306 ± 0.322
4.193 ± 0.353
4.225 ± 0.287
6.072 ± 0.298
6.604 ± 0.307
6.572 ± 0.242
1.355 ± 0.123
3.935 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here