Junonia coenia densovirus (isolate pBRJ/1990) (JcDNV)
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q90053-2|CAPSD-2_JDNVP Isoform of Q90053 Isoform VP2 of Capsid protein VP1 OS=Junonia coenia densovirus (isolate pBRJ/1990) OX=648250 GN=VP PE=4 SV=1
MM1 pKa = 7.22 QHH3 pKa = 7.19 DD4 pKa = 5.37 LDD6 pKa = 4.12 NQIIACGEE14 pKa = 4.68 DD15 pKa = 3.31 IDD17 pKa = 4.49 HH18 pKa = 6.43 TLAMEE23 pKa = 4.49 EE24 pKa = 4.49 LEE26 pKa = 4.07 HH27 pKa = 7.24 WDD29 pKa = 2.94 WTKK32 pKa = 11.08 QNRR35 pKa = 11.84 LPFQLYY41 pKa = 9.49 LAVMHH46 pKa = 6.64 LNEE49 pKa = 4.17 IPEE52 pKa = 4.24 WLDD55 pKa = 2.89 EE56 pKa = 4.26 TMLIEE61 pKa = 3.85 CVYY64 pKa = 10.39 YY65 pKa = 10.45 FKK67 pKa = 11.1 EE68 pKa = 4.67 LINHH72 pKa = 6.96 RR73 pKa = 11.84 DD74 pKa = 3.5 PYY76 pKa = 10.9 DD77 pKa = 3.56 TDD79 pKa = 4.78 EE80 pKa = 4.42 FNAWNMNGKK89 pKa = 8.73 PFKK92 pKa = 10.53 TMWKK96 pKa = 8.76 ICKK99 pKa = 9.35 FCYY102 pKa = 9.5 TNCEE106 pKa = 4.11 DD107 pKa = 3.9 PDD109 pKa = 3.92 EE110 pKa = 4.09 YY111 pKa = 11.39 RR112 pKa = 11.84 FMYY115 pKa = 10.02 NRR117 pKa = 11.84 TVFVEE122 pKa = 4.08 DD123 pKa = 4.35 AEE125 pKa = 5.28 DD126 pKa = 3.96 IINRR130 pKa = 11.84 LQDD133 pKa = 3.38 GSSWCQICHH142 pKa = 6.19 TCPLFNISVIYY153 pKa = 10.32 EE154 pKa = 3.68 NSPNKK159 pKa = 9.24 KK160 pKa = 9.17 RR161 pKa = 11.84 RR162 pKa = 11.84 YY163 pKa = 8.71 SSSSEE168 pKa = 3.39 EE169 pKa = 4.04 DD170 pKa = 3.19 EE171 pKa = 4.36 YY172 pKa = 11.21 MSNSFYY178 pKa = 11.24 VKK180 pKa = 10.26 HH181 pKa = 6.3 PNSRR185 pKa = 11.84 HH186 pKa = 4.18
Molecular weight: 22.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.5
IPC2_protein 4.711
IPC_protein 4.647
Toseland 4.533
ProMoST 4.749
Dawson 4.622
Bjellqvist 4.762
Wikipedia 4.482
Rodwell 4.52
Grimsley 4.444
Solomon 4.609
Lehninger 4.571
Nozaki 4.724
DTASelect 4.876
Thurlkill 4.533
EMBOSS 4.507
Sillero 4.787
Patrickios 1.392
IPC_peptide 4.622
IPC2_peptide 4.787
IPC2.peptide.svr19 4.683
Protein with the highest isoelectric point:
>sp|Q90053-4|CAPSD-4_JDNVP Isoform of Q90053 Isoform VP4 of Capsid protein VP1 OS=Junonia coenia densovirus (isolate pBRJ/1990) OX=648250 GN=VP PE=4 SV=1
MM1 pKa = 7.58 SLPGTGSGTSSGGGNTSGQEE21 pKa = 4.0 VYY23 pKa = 10.71 VIPRR27 pKa = 11.84 PFSNFGKK34 pKa = 10.62 KK35 pKa = 10.08 LSTYY39 pKa = 7.81 TKK41 pKa = 8.62 SHH43 pKa = 6.28 KK44 pKa = 10.63 FMIFGLANNVIGPTGTGTTAVNRR67 pKa = 11.84 LITTCLAEE75 pKa = 4.61 IPWQKK80 pKa = 10.89 LPLYY84 pKa = 8.6 MNQSEE89 pKa = 4.69 FDD91 pKa = 4.02 LLPPGSRR98 pKa = 11.84 VVEE101 pKa = 4.39 CNVKK105 pKa = 10.69 VIFRR109 pKa = 11.84 TNRR112 pKa = 11.84 IAFEE116 pKa = 4.15 TSSTATKK123 pKa = 10.31 QATLNQISNLQTAVGLNKK141 pKa = 10.33 LGWGIDD147 pKa = 3.23 RR148 pKa = 11.84 SFTAFQSDD156 pKa = 3.51 QPMIPTATSAPKK168 pKa = 10.21 YY169 pKa = 10.12 EE170 pKa = 5.64 PITGTTGYY178 pKa = 10.42 RR179 pKa = 11.84 GMIADD184 pKa = 4.09 YY185 pKa = 11.0 YY186 pKa = 11.09 GADD189 pKa = 3.73 STNDD193 pKa = 3.38 AAFGNAGNYY202 pKa = 7.38 PHH204 pKa = 6.82 HH205 pKa = 6.4 QVGSFTFIQNYY216 pKa = 7.86 YY217 pKa = 11.13 CMYY220 pKa = 9.91 QQTNQGTGGWPCLAEE235 pKa = 4.26 HH236 pKa = 6.78 LQQFDD241 pKa = 3.76 SKK243 pKa = 9.86 TVNNQCLIDD252 pKa = 3.78 VTYY255 pKa = 9.34 KK256 pKa = 10.87 PKK258 pKa = 10.0 MGLIKK263 pKa = 10.54 PPLNYY268 pKa = 10.1 KK269 pKa = 10.37 IIGQPTAKK277 pKa = 9.41 GTISVGDD284 pKa = 3.66 NLVNMRR290 pKa = 11.84 GAVVINPPEE299 pKa = 4.13 ATQSVTEE306 pKa = 4.27 STHH309 pKa = 5.8 NLTRR313 pKa = 11.84 NFPANLFNIYY323 pKa = 10.6 SDD325 pKa = 3.59 IEE327 pKa = 4.02 KK328 pKa = 10.69 SQILHH333 pKa = 6.25 KK334 pKa = 10.78 GPWGHH339 pKa = 6.63 EE340 pKa = 3.99 NPQIQPSVHH349 pKa = 7.13 IGIQAVPALTTGALLVNSSPLNSWTDD375 pKa = 3.1 SMGYY379 pKa = 8.99 IDD381 pKa = 5.55 VMSSCTVMEE390 pKa = 4.21 SQPTHH395 pKa = 6.45 FPFSTDD401 pKa = 2.82 ANTNPGNTIYY411 pKa = 10.77 RR412 pKa = 11.84 INLTPNSLTSAFNGLYY428 pKa = 10.89 GNGATLGNVV437 pKa = 3.2
Molecular weight: 47.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.096
IPC2_protein 7.249
IPC_protein 7.249
Toseland 7.015
ProMoST 7.717
Dawson 7.878
Bjellqvist 8.112
Wikipedia 7.805
Rodwell 7.878
Grimsley 7.102
Solomon 7.966
Lehninger 7.98
Nozaki 8.273
DTASelect 7.995
Thurlkill 8.039
EMBOSS 8.112
Sillero 8.317
Patrickios 3.579
IPC_peptide 7.966
IPC2_peptide 7.38
IPC2.peptide.svr19 7.388
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
3
7
3274
186
810
467.7
51.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.864 ± 0.543
1.374 ± 0.292
4.368 ± 0.56
5.192 ± 0.748
3.971 ± 0.419
7.697 ± 0.95
2.23 ± 0.182
5.987 ± 0.156
5.192 ± 0.487
6.964 ± 0.303
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.535 ± 0.228
7.575 ± 0.353
5.834 ± 0.497
4.673 ± 0.309
3.451 ± 0.654
8.308 ± 0.418
8.369 ± 0.813
4.918 ± 0.227
1.374 ± 0.265
4.032 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here