Pelagibacter phage HTVC011P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Stopavirus; Pelagibacter virus HTVC011P

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1I885|M1I885_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC011P OX=1283078 PE=4 SV=1
MM1 pKa = 6.7TQYY4 pKa = 11.75AFNTYY9 pKa = 8.45TGNGSTTQYY18 pKa = 10.49SISFSYY24 pKa = 10.51IDD26 pKa = 3.64STHH29 pKa = 5.73VKK31 pKa = 10.12CFLDD35 pKa = 4.26GVEE38 pKa = 4.54TSAFTISSSTVTFNTAPTNSSVIRR62 pKa = 11.84IEE64 pKa = 3.81RR65 pKa = 11.84QTPVDD70 pKa = 3.48SRR72 pKa = 11.84LVDD75 pKa = 4.21FQDD78 pKa = 3.99GSVLTEE84 pKa = 4.27AEE86 pKa = 4.39LDD88 pKa = 3.35MSANQNFYY96 pKa = 10.95AVQEE100 pKa = 4.28ITDD103 pKa = 3.93DD104 pKa = 3.5QSNNLGLTTADD115 pKa = 4.08VYY117 pKa = 11.31DD118 pKa = 4.45ANNKK122 pKa = 10.0RR123 pKa = 11.84IINVADD129 pKa = 3.7PVDD132 pKa = 3.57NTDD135 pKa = 3.33AVNKK139 pKa = 9.92QFISTNLPNITTVAGISADD158 pKa = 3.48VTTVAGISADD168 pKa = 3.58VTAVANDD175 pKa = 3.36ATDD178 pKa = 3.12IGVVSTNIASVNTVATNIADD198 pKa = 4.11VIKK201 pKa = 11.04VADD204 pKa = 4.79DD205 pKa = 3.71LNEE208 pKa = 4.8AISEE212 pKa = 4.4VEE214 pKa = 4.24TVANDD219 pKa = 3.58LNEE222 pKa = 4.22ATSEE226 pKa = 4.16IEE228 pKa = 4.1VVANNIVNVNTVGTINADD246 pKa = 3.27VTTVANNEE254 pKa = 3.83TDD256 pKa = 3.26IQTLADD262 pKa = 4.24LEE264 pKa = 5.04DD265 pKa = 4.01GTVTTNGLSTLAGLNTEE282 pKa = 3.89IQGVYY287 pKa = 10.52NIRR290 pKa = 11.84TNVTNVDD297 pKa = 3.54TNATNVNLVAGQISPTNNVSAVSAVATEE325 pKa = 3.92IGTLGALGTEE335 pKa = 3.91ITNLNNIRR343 pKa = 11.84TDD345 pKa = 2.9ISGVNTISAEE355 pKa = 4.07VTAVNNNSTNINTVAGLDD373 pKa = 3.74TEE375 pKa = 4.45ITALGASGTVASINTVASNLASVNNFGEE403 pKa = 4.61VYY405 pKa = 10.0RR406 pKa = 11.84ISATAPTTSLDD417 pKa = 4.65DD418 pKa = 3.64GDD420 pKa = 3.69MWFDD424 pKa = 3.38TTADD428 pKa = 3.93KK429 pKa = 11.3LKK431 pKa = 9.82IWNGSSFDD439 pKa = 4.06LAGSSINGTSARR451 pKa = 11.84FKK453 pKa = 10.58YY454 pKa = 10.27VATAGQTTFTGADD467 pKa = 3.31ANGEE471 pKa = 4.13TLAYY475 pKa = 7.87DD476 pKa = 3.59TGYY479 pKa = 10.53IDD481 pKa = 5.3AYY483 pKa = 11.15LNGVHH488 pKa = 7.25LDD490 pKa = 3.62PSDD493 pKa = 3.59YY494 pKa = 10.66TATDD498 pKa = 3.45GSNFVLSSGATVSDD512 pKa = 3.4EE513 pKa = 4.28LYY515 pKa = 10.1IVAFGTFSVANIDD528 pKa = 3.74ASNISAGTINEE539 pKa = 3.83ARR541 pKa = 11.84LPTITNAKK549 pKa = 9.29LDD551 pKa = 3.8NSSITINGSAVNLGGSVTVGEE572 pKa = 4.52TKK574 pKa = 9.18PTISSISPDD583 pKa = 3.97TITNAQTSITITGSNFVSVPQVEE606 pKa = 4.51FLNPSTGIWYY616 pKa = 9.82VADD619 pKa = 3.22TVTYY623 pKa = 10.71NNSTSLTVNATLGVDD638 pKa = 3.47GTIQNKK644 pKa = 8.43NRR646 pKa = 11.84EE647 pKa = 4.3PRR649 pKa = 11.84WFSNIIWNNTYY660 pKa = 10.12SFRR663 pKa = 11.84CTNMEE668 pKa = 4.22DD669 pKa = 3.79CCWRR673 pKa = 11.84YY674 pKa = 10.01

Molecular weight: 70.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1HM84|M1HM84_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC011P OX=1283078 PE=4 SV=1
MM1 pKa = 7.49SNATTKK7 pKa = 10.55QKK9 pKa = 11.26APTEE13 pKa = 4.13ASTDD17 pKa = 3.23ASRR20 pKa = 11.84DD21 pKa = 3.4TTVASNYY28 pKa = 7.92SRR30 pKa = 11.84KK31 pKa = 9.42RR32 pKa = 11.84VGRR35 pKa = 11.84GSLRR39 pKa = 11.84IPLSGGSGLNFPTSS53 pKa = 3.19

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

12316

41

1230

273.7

30.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.504 ± 0.3

0.796 ± 0.165

5.83 ± 0.242

6.025 ± 0.417

4.117 ± 0.228

6.398 ± 0.37

1.575 ± 0.201

6.983 ± 0.291

8.469 ± 0.652

8.071 ± 0.383

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.135 ± 0.187

6.699 ± 0.375

2.939 ± 0.162

3.979 ± 0.309

3.784 ± 0.262

7.616 ± 0.57

7.584 ± 0.65

5.781 ± 0.373

1.056 ± 0.139

3.662 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski