Firmicutes bacterium CAG:227

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2286 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6VHE7|R6VHE7_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:227 OX=1263010 GN=BN546_01302 PE=4 SV=1
MM1 pKa = 8.22SEE3 pKa = 3.94EE4 pKa = 4.32TFTTNIGGCDD14 pKa = 3.52PSDD17 pKa = 3.91CASCTSDD24 pKa = 4.24CAGAGQAIPRR34 pKa = 11.84VPNPTIKK41 pKa = 10.01LTLEE45 pKa = 4.35DD46 pKa = 5.31DD47 pKa = 4.2SVLEE51 pKa = 4.26CAVLTTFDD59 pKa = 3.51VEE61 pKa = 4.92GFGEE65 pKa = 4.75YY66 pKa = 9.62IALIPLDD73 pKa = 3.91KK74 pKa = 10.95NGKK77 pKa = 8.12NKK79 pKa = 10.52SGEE82 pKa = 4.22TFLFRR87 pKa = 11.84YY88 pKa = 9.81HH89 pKa = 7.04EE90 pKa = 4.63EE91 pKa = 3.95NGNPVINNIEE101 pKa = 4.03RR102 pKa = 11.84EE103 pKa = 4.22DD104 pKa = 3.89EE105 pKa = 4.05YY106 pKa = 11.08MAAAEE111 pKa = 4.15SFQNFIEE118 pKa = 4.33NLPHH122 pKa = 6.54EE123 pKa = 4.62NEE125 pKa = 3.87IDD127 pKa = 3.73EE128 pKa = 5.07DD129 pKa = 4.45PEE131 pKa = 4.41DD132 pKa = 4.2EE133 pKa = 4.85PGEE136 pKa = 4.11EE137 pKa = 3.79QDD139 pKa = 3.41EE140 pKa = 4.99DD141 pKa = 3.75IFAEE145 pKa = 4.25

Molecular weight: 16.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6V1A8|R6V1A8_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:227 OX=1263010 GN=BN546_01560 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.69VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2286

0

2286

757468

32

2920

331.4

37.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.178 ± 0.052

1.496 ± 0.024

5.282 ± 0.042

8.455 ± 0.07

4.074 ± 0.036

6.984 ± 0.047

1.725 ± 0.022

7.322 ± 0.045

7.721 ± 0.048

8.87 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.07 ± 0.029

4.391 ± 0.037

3.15 ± 0.027

3.326 ± 0.027

3.886 ± 0.037

5.756 ± 0.049

5.372 ± 0.042

6.935 ± 0.038

0.9 ± 0.016

4.106 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski