Amino acid dipepetide frequency for Firmicutes bacterium CAG:227

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.508AlaAla: 5.508 ± 0.123	1.093AlaCys: 1.093 ± 0.041	4.101AlaAsp: 4.101 ± 0.083	5.361AlaGlu: 5.361 ± 0.106	3.013AlaPhe: 3.013 ± 0.077	5.889AlaGly: 5.889 ± 0.114	1.104AlaHis: 1.104 ± 0.04	4.932AlaIle: 4.932 ± 0.091	5.679AlaLys: 5.679 ± 0.106	6.668AlaLeu: 6.668 ± 0.096	2.364AlaMet: 2.364 ± 0.061	2.574AlaAsn: 2.574 ± 0.058	1.91AlaPro: 1.91 ± 0.052	2.317AlaGln: 2.317 ± 0.06	2.578AlaArg: 2.578 ± 0.061	3.97AlaSer: 3.97 ± 0.07	3.194AlaThr: 3.194 ± 0.091	6.271AlaVal: 6.271 ± 0.118	0.558AlaTrp: 0.558 ± 0.031	2.692AlaTyr: 2.692 ± 0.064	0.001AlaXaa: 0.001 ± 0.001
Cys	1.002CysAla: 1.002 ± 0.037	0.302CysCys: 0.302 ± 0.022	0.767CysAsp: 0.767 ± 0.036	1.007CysGlu: 1.007 ± 0.038	0.68CysPhe: 0.68 ± 0.031	1.497CysGly: 1.497 ± 0.052	0.322CysHis: 0.322 ± 0.023	1.075CysIle: 1.075 ± 0.036	0.974CysLys: 0.974 ± 0.037	1.204CysLeu: 1.204 ± 0.041	0.459CysMet: 0.459 ± 0.023	0.582CysAsn: 0.582 ± 0.024	0.642CysPro: 0.642 ± 0.037	0.455CysGln: 0.455 ± 0.025	0.569CysArg: 0.569 ± 0.028	0.911CysSer: 0.911 ± 0.042	0.747CysThr: 0.747 ± 0.035	1.073CysVal: 1.073 ± 0.039	0.106CysTrp: 0.106 ± 0.012	0.581CysTyr: 0.581 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	4.037AspAla: 4.037 ± 0.079	0.803AspCys: 0.803 ± 0.034	2.507AspAsp: 2.507 ± 0.069	4.916AspGlu: 4.916 ± 0.092	2.537AspPhe: 2.537 ± 0.057	4.359AspGly: 4.359 ± 0.103	0.648AspHis: 0.648 ± 0.033	3.884AspIle: 3.884 ± 0.069	3.724AspLys: 3.724 ± 0.08	4.388AspLeu: 4.388 ± 0.077	1.609AspMet: 1.609 ± 0.051	2.096AspAsn: 2.096 ± 0.068	1.34AspPro: 1.34 ± 0.044	1.044AspGln: 1.044 ± 0.037	1.815AspArg: 1.815 ± 0.047	3.141AspSer: 3.141 ± 0.087	2.992AspThr: 2.992 ± 0.072	3.805AspVal: 3.805 ± 0.077	0.585AspTrp: 0.585 ± 0.031	2.59AspTyr: 2.59 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	6.235GluAla: 6.235 ± 0.116	0.927GluCys: 0.927 ± 0.034	4.251GluAsp: 4.251 ± 0.086	9.355GluGlu: 9.355 ± 0.157	2.951GluPhe: 2.951 ± 0.068	4.493GluGly: 4.493 ± 0.078	1.435GluHis: 1.435 ± 0.04	6.333GluIle: 6.333 ± 0.098	8.148GluLys: 8.148 ± 0.119	7.051GluLeu: 7.051 ± 0.109	2.598GluMet: 2.598 ± 0.061	4.792GluAsn: 4.792 ± 0.092	1.867GluPro: 1.867 ± 0.051	3.13GluGln: 3.13 ± 0.074	3.279GluArg: 3.279 ± 0.073	3.794GluSer: 3.794 ± 0.077	4.63GluThr: 4.63 ± 0.086	5.244GluVal: 5.244 ± 0.089	0.673GluTrp: 0.673 ± 0.035	3.364GluTyr: 3.364 ± 0.076	0.0GluXaa: 0.0 ± 0.0
Phe	2.836PheAla: 2.836 ± 0.068	0.739PheCys: 0.739 ± 0.032	2.496PheAsp: 2.496 ± 0.064	3.071PheGlu: 3.071 ± 0.063	1.943PhePhe: 1.943 ± 0.06	3.062PheGly: 3.062 ± 0.077	0.85PheHis: 0.85 ± 0.033	2.695PheIle: 2.695 ± 0.062	2.189PheLys: 2.189 ± 0.05	4.066PheLeu: 4.066 ± 0.088	1.17PheMet: 1.17 ± 0.042	1.451PheAsn: 1.451 ± 0.045	1.414PhePro: 1.414 ± 0.05	1.259PheGln: 1.259 ± 0.044	1.498PheArg: 1.498 ± 0.052	2.858PheSer: 2.858 ± 0.067	2.201PheThr: 2.201 ± 0.059	2.819PheVal: 2.819 ± 0.068	0.424PheTrp: 0.424 ± 0.024	1.699PheTyr: 1.699 ± 0.058	0.0PheXaa: 0.0 ± 0.0
Gly	4.988GlyAla: 4.988 ± 0.097	1.304GlyCys: 1.304 ± 0.048	3.217GlyAsp: 3.217 ± 0.072	4.905GlyGlu: 4.905 ± 0.091	3.085GlyPhe: 3.085 ± 0.072	4.626GlyGly: 4.626 ± 0.103	1.278GlyHis: 1.278 ± 0.042	6.367GlyIle: 6.367 ± 0.109	5.834GlyLys: 5.834 ± 0.096	5.501GlyLeu: 5.501 ± 0.096	2.436GlyMet: 2.436 ± 0.07	2.998GlyAsn: 2.998 ± 0.083	1.322GlyPro: 1.322 ± 0.046	1.946GlyGln: 1.946 ± 0.055	2.636GlyArg: 2.636 ± 0.062	3.945GlySer: 3.945 ± 0.086	4.38GlyThr: 4.38 ± 0.097	5.044GlyVal: 5.044 ± 0.085	0.821GlyTrp: 0.821 ± 0.052	3.209GlyTyr: 3.209 ± 0.071	0.001GlyXaa: 0.001 ± 0.001
His	1.104HisAla: 1.104 ± 0.042	0.302HisCys: 0.302 ± 0.02	0.78HisAsp: 0.78 ± 0.037	1.129HisGlu: 1.129 ± 0.042	0.841HisPhe: 0.841 ± 0.033	1.289HisGly: 1.289 ± 0.051	0.436HisHis: 0.436 ± 0.028	1.254HisIle: 1.254 ± 0.045	1.166HisLys: 1.166 ± 0.035	1.6HisLeu: 1.6 ± 0.052	0.375HisMet: 0.375 ± 0.024	0.73HisAsn: 0.73 ± 0.029	0.857HisPro: 0.857 ± 0.038	0.609HisGln: 0.609 ± 0.031	0.726HisArg: 0.726 ± 0.033	0.978HisSer: 0.978 ± 0.038	1.026HisThr: 1.026 ± 0.033	1.085HisVal: 1.085 ± 0.039	0.182HisTrp: 0.182 ± 0.017	0.782HisTyr: 0.782 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.344IleAla: 5.344 ± 0.101	1.327IleCys: 1.327 ± 0.049	3.962IleAsp: 3.962 ± 0.075	5.532IleGlu: 5.532 ± 0.093	2.955IlePhe: 2.955 ± 0.071	5.099IleGly: 5.099 ± 0.101	1.405IleHis: 1.405 ± 0.044	4.708IleIle: 4.708 ± 0.091	4.614IleLys: 4.614 ± 0.083	7.401IleLeu: 7.401 ± 0.134	1.986IleMet: 1.986 ± 0.051	2.755IleAsn: 2.755 ± 0.075	3.142IlePro: 3.142 ± 0.067	2.541IleGln: 2.541 ± 0.06	3.154IleArg: 3.154 ± 0.071	4.881IleSer: 4.881 ± 0.085	3.97IleThr: 3.97 ± 0.088	4.912IleVal: 4.912 ± 0.091	0.665IleTrp: 0.665 ± 0.029	2.869IleTyr: 2.869 ± 0.065	0.0IleXaa: 0.0 ± 0.0
Lys	5.546LysAla: 5.546 ± 0.112	0.829LysCys: 0.829 ± 0.043	4.057LysAsp: 4.057 ± 0.079	8.927LysGlu: 8.927 ± 0.144	2.12LysPhe: 2.12 ± 0.059	4.511LysGly: 4.511 ± 0.082	1.036LysHis: 1.036 ± 0.037	5.71LysIle: 5.71 ± 0.093	7.611LysLys: 7.611 ± 0.124	5.664LysLeu: 5.664 ± 0.098	2.622LysMet: 2.622 ± 0.056	4.229LysAsn: 4.229 ± 0.073	2.04LysPro: 2.04 ± 0.056	2.742LysGln: 2.742 ± 0.072	3.261LysArg: 3.261 ± 0.065	3.695LysSer: 3.695 ± 0.075	4.244LysThr: 4.244 ± 0.087	4.858LysVal: 4.858 ± 0.08	0.672LysTrp: 0.672 ± 0.035	2.834LysTyr: 2.834 ± 0.064	0.001LysXaa: 0.001 ± 0.001
Leu	6.527LeuAla: 6.527 ± 0.116	1.432LeuCys: 1.432 ± 0.047	4.779LeuAsp: 4.779 ± 0.077	7.266LeuGlu: 7.266 ± 0.131	3.772LeuPhe: 3.772 ± 0.081	6.066LeuGly: 6.066 ± 0.102	1.564LeuHis: 1.564 ± 0.049	5.984LeuIle: 5.984 ± 0.103	7.083LeuLys: 7.083 ± 0.092	8.296LeuLeu: 8.296 ± 0.144	2.535LeuMet: 2.535 ± 0.057	3.995LeuAsn: 3.995 ± 0.076	3.372LeuPro: 3.372 ± 0.067	2.576LeuGln: 2.576 ± 0.055	3.232LeuArg: 3.232 ± 0.077	5.976LeuSer: 5.976 ± 0.105	4.696LeuThr: 4.696 ± 0.087	5.644LeuVal: 5.644 ± 0.091	0.725LeuTrp: 0.725 ± 0.032	3.179LeuTyr: 3.179 ± 0.075	0.0LeuXaa: 0.0 ± 0.0
Met	2.322MetAla: 2.322 ± 0.059	0.317MetCys: 0.317 ± 0.021	1.731MetAsp: 1.731 ± 0.042	2.636MetGlu: 2.636 ± 0.062	0.945MetPhe: 0.945 ± 0.035	2.119MetGly: 2.119 ± 0.058	0.474MetHis: 0.474 ± 0.027	2.294MetIle: 2.294 ± 0.064	2.671MetLys: 2.671 ± 0.058	2.632MetLeu: 2.632 ± 0.064	1.002MetMet: 1.002 ± 0.035	1.505MetAsn: 1.505 ± 0.054	1.167MetPro: 1.167 ± 0.038	1.15MetGln: 1.15 ± 0.041	1.267MetArg: 1.267 ± 0.043	1.741MetSer: 1.741 ± 0.051	1.658MetThr: 1.658 ± 0.045	1.921MetVal: 1.921 ± 0.054	0.207MetTrp: 0.207 ± 0.017	0.943MetTyr: 0.943 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	3.06AsnAla: 3.06 ± 0.068	0.66AsnCys: 0.66 ± 0.031	1.996AsnAsp: 1.996 ± 0.064	3.147AsnGlu: 3.147 ± 0.078	1.671AsnPhe: 1.671 ± 0.051	3.571AsnGly: 3.571 ± 0.084	0.874AsnHis: 0.874 ± 0.034	3.058AsnIle: 3.058 ± 0.065	2.935AsnLys: 2.935 ± 0.065	3.925AsnLeu: 3.925 ± 0.08	1.364AsnMet: 1.364 ± 0.043	1.733AsnAsn: 1.733 ± 0.074	2.118AsnPro: 2.118 ± 0.053	1.962AsnGln: 1.962 ± 0.056	1.945AsnArg: 1.945 ± 0.061	2.417AsnSer: 2.417 ± 0.075	2.36AsnThr: 2.36 ± 0.069	2.778AsnVal: 2.778 ± 0.061	0.448AsnTrp: 0.448 ± 0.022	1.884AsnTyr: 1.884 ± 0.05	0.0AsnXaa: 0.0 ± 0.0
Pro	2.263ProAla: 2.263 ± 0.062	0.467ProCys: 0.467 ± 0.026	2.119ProAsp: 2.119 ± 0.05	3.241ProGlu: 3.241 ± 0.074	1.509ProPhe: 1.509 ± 0.043	2.046ProGly: 2.046 ± 0.054	0.557ProHis: 0.557 ± 0.03	2.19ProIle: 2.19 ± 0.054	2.141ProLys: 2.141 ± 0.059	2.622ProLeu: 2.622 ± 0.063	0.838ProMet: 0.838 ± 0.034	1.325ProAsn: 1.325 ± 0.043	0.619ProPro: 0.619 ± 0.032	1.011ProGln: 1.011 ± 0.036	0.854ProArg: 0.854 ± 0.035	1.7ProSer: 1.7 ± 0.051	1.43ProThr: 1.43 ± 0.049	2.77ProVal: 2.77 ± 0.06	0.327ProTrp: 0.327 ± 0.024	1.468ProTyr: 1.468 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	2.243GlnAla: 2.243 ± 0.069	0.343GlnCys: 0.343 ± 0.02	1.646GlnAsp: 1.646 ± 0.048	3.081GlnGlu: 3.081 ± 0.073	1.166GlnPhe: 1.166 ± 0.041	1.939GlnGly: 1.939 ± 0.05	0.448GlnHis: 0.448 ± 0.024	2.858GlnIle: 2.858 ± 0.066	3.211GlnLys: 3.211 ± 0.072	2.852GlnLeu: 2.852 ± 0.079	1.186GlnMet: 1.186 ± 0.045	1.848GlnAsn: 1.848 ± 0.058	0.815GlnPro: 0.815 ± 0.035	1.117GlnGln: 1.117 ± 0.047	1.281GlnArg: 1.281 ± 0.046	1.708GlnSer: 1.708 ± 0.052	1.757GlnThr: 1.757 ± 0.049	2.073GlnVal: 2.073 ± 0.049	0.293GlnTrp: 0.293 ± 0.021	1.399GlnTyr: 1.399 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	2.445ArgAla: 2.445 ± 0.056	0.521ArgCys: 0.521 ± 0.029	1.788ArgAsp: 1.788 ± 0.05	3.502ArgGlu: 3.502 ± 0.083	1.666ArgPhe: 1.666 ± 0.047	2.309ArgGly: 2.309 ± 0.061	0.702ArgHis: 0.702 ± 0.033	3.257ArgIle: 3.257 ± 0.081	3.456ArgLys: 3.456 ± 0.068	3.44ArgLeu: 3.44 ± 0.076	1.432ArgMet: 1.432 ± 0.047	1.76ArgAsn: 1.76 ± 0.053	1.154ArgPro: 1.154 ± 0.039	1.455ArgGln: 1.455 ± 0.045	1.749ArgArg: 1.749 ± 0.055	1.793ArgSer: 1.793 ± 0.056	1.938ArgThr: 1.938 ± 0.049	2.469ArgVal: 2.469 ± 0.066	0.33ArgTrp: 0.33 ± 0.024	1.689ArgTyr: 1.689 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	4.313SerAla: 4.313 ± 0.091	0.887SerCys: 0.887 ± 0.037	3.368SerAsp: 3.368 ± 0.076	4.32SerGlu: 4.32 ± 0.088	2.465SerPhe: 2.465 ± 0.059	4.668SerGly: 4.668 ± 0.08	0.993SerHis: 0.993 ± 0.037	4.104SerIle: 4.104 ± 0.074	3.769SerLys: 3.769 ± 0.076	5.013SerLeu: 5.013 ± 0.098	1.836SerMet: 1.836 ± 0.05	2.351SerAsn: 2.351 ± 0.072	1.634SerPro: 1.634 ± 0.048	1.91SerGln: 1.91 ± 0.061	2.276SerArg: 2.276 ± 0.049	3.698SerSer: 3.698 ± 0.092	2.667SerThr: 2.667 ± 0.07	4.279SerVal: 4.279 ± 0.076	0.502SerTrp: 0.502 ± 0.027	2.504SerTyr: 2.504 ± 0.069	0.0SerXaa: 0.0 ± 0.0
Thr	4.226ThrAla: 4.226 ± 0.074	0.667ThrCys: 0.667 ± 0.029	2.969ThrAsp: 2.969 ± 0.073	3.889ThrGlu: 3.889 ± 0.085	2.126ThrPhe: 2.126 ± 0.059	4.569ThrGly: 4.569 ± 0.096	0.867ThrHis: 0.867 ± 0.038	4.019ThrIle: 4.019 ± 0.08	3.753ThrLys: 3.753 ± 0.075	4.939ThrLeu: 4.939 ± 0.091	1.421ThrMet: 1.421 ± 0.049	2.087ThrAsn: 2.087 ± 0.058	2.001ThrPro: 2.001 ± 0.052	1.616ThrGln: 1.616 ± 0.046	1.79ThrArg: 1.79 ± 0.05	2.941ThrSer: 2.941 ± 0.066	2.914ThrThr: 2.914 ± 0.086	4.357ThrVal: 4.357 ± 0.089	0.416ThrTrp: 0.416 ± 0.027	2.155ThrTyr: 2.155 ± 0.069	0.0ThrXaa: 0.0 ± 0.0
Val	4.623ValAla: 4.623 ± 0.108	1.275ValCys: 1.275 ± 0.048	3.5ValAsp: 3.5 ± 0.065	5.25ValGlu: 5.25 ± 0.092	3.047ValPhe: 3.047 ± 0.066	4.326ValGly: 4.326 ± 0.081	1.126ValHis: 1.126 ± 0.039	5.302ValIle: 5.302 ± 0.095	4.994ValLys: 4.994 ± 0.087	6.7ValLeu: 6.7 ± 0.104	1.959ValMet: 1.959 ± 0.053	2.863ValAsn: 2.863 ± 0.069	2.423ValPro: 2.423 ± 0.053	2.155ValGln: 2.155 ± 0.059	2.792ValArg: 2.792 ± 0.08	4.576ValSer: 4.576 ± 0.081	4.247ValThr: 4.247 ± 0.088	4.865ValVal: 4.865 ± 0.089	0.62ValTrp: 0.62 ± 0.031	2.704ValTyr: 2.704 ± 0.059	0.0ValXaa: 0.0 ± 0.0
Trp	0.5TrpAla: 0.5 ± 0.029	0.129TrpCys: 0.129 ± 0.013	0.446TrpAsp: 0.446 ± 0.026	0.694TrpGlu: 0.694 ± 0.035	0.408TrpPhe: 0.408 ± 0.022	0.581TrpGly: 0.581 ± 0.029	0.169TrpHis: 0.169 ± 0.016	0.716TrpIle: 0.716 ± 0.03	0.796TrpLys: 0.796 ± 0.04	0.816TrpLeu: 0.816 ± 0.033	0.384TrpMet: 0.384 ± 0.024	0.506TrpAsn: 0.506 ± 0.03	0.205TrpPro: 0.205 ± 0.017	0.411TrpGln: 0.411 ± 0.034	0.338TrpArg: 0.338 ± 0.021	0.485TrpSer: 0.485 ± 0.028	0.371TrpThr: 0.371 ± 0.024	0.507TrpVal: 0.507 ± 0.03	0.098TrpTrp: 0.098 ± 0.012	0.444TrpTyr: 0.444 ± 0.035	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.656TyrAla: 2.656 ± 0.058	0.63TyrCys: 0.63 ± 0.031	2.343TyrAsp: 2.343 ± 0.057	3.319TyrGlu: 3.319 ± 0.065	1.84TyrPhe: 1.84 ± 0.055	2.824TyrGly: 2.824 ± 0.068	0.961TyrHis: 0.961 ± 0.039	2.481TyrIle: 2.481 ± 0.054	2.461TyrLys: 2.461 ± 0.067	3.921TyrLeu: 3.921 ± 0.084	1.125TyrMet: 1.125 ± 0.046	1.723TyrAsn: 1.723 ± 0.056	1.46TyrPro: 1.46 ± 0.042	1.858TyrGln: 1.858 ± 0.047	1.814TyrArg: 1.814 ± 0.049	2.347TyrSer: 2.347 ± 0.061	2.3TyrThr: 2.3 ± 0.068	2.576TyrVal: 2.576 ± 0.065	0.35TyrTrp: 0.35 ± 0.031	2.075TyrTyr: 2.075 ± 0.078	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.004XaaXaa: 0.004 ± 0.003

Statistics based on 2286 proteins (757468 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---