Sanxia atyid shrimp virus 4

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Jingchuvirales; Chuviridae; Piscichuvirus; Piscichuvirus sanxiaense

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KN13|A0A1L3KN13_9VIRU Uncharacterized protein OS=Sanxia atyid shrimp virus 4 OX=1923358 PE=4 SV=1
MM1 pKa = 7.36NFFRR5 pKa = 11.84SKK7 pKa = 10.58PSGTGVRR14 pKa = 11.84NRR16 pKa = 11.84GHH18 pKa = 7.19AFTGAVAPLVVTHH31 pKa = 7.04RR32 pKa = 11.84VHH34 pKa = 6.59QRR36 pKa = 11.84GICEE40 pKa = 3.74NAEE43 pKa = 4.15TFGVLGGGLSSLSQLVFRR61 pKa = 11.84NIDD64 pKa = 3.37VPPFTTARR72 pKa = 11.84RR73 pKa = 11.84DD74 pKa = 3.44AEE76 pKa = 4.18EE77 pKa = 4.37FGLKK81 pKa = 9.51EE82 pKa = 3.93FYY84 pKa = 10.73AIVLFCTSRR93 pKa = 11.84APSRR97 pKa = 11.84PSRR100 pKa = 11.84LLLSIAEE107 pKa = 4.12GLMPNMPQDD116 pKa = 3.19RR117 pKa = 11.84FLITAEE123 pKa = 3.82SRR125 pKa = 11.84RR126 pKa = 11.84VFVQIMRR133 pKa = 11.84DD134 pKa = 2.94TWPEE138 pKa = 3.49HH139 pKa = 6.0KK140 pKa = 10.71ANARR144 pKa = 11.84AWLADD149 pKa = 3.28AKK151 pKa = 11.03ANRR154 pKa = 11.84FAANDD159 pKa = 3.98HH160 pKa = 6.86IINGTYY166 pKa = 10.66DD167 pKa = 3.3LGARR171 pKa = 11.84VTPDD175 pKa = 3.05FLMEE179 pKa = 4.03VLMVEE184 pKa = 4.77ASLIDD189 pKa = 3.3EE190 pKa = 4.51GEE192 pKa = 3.93RR193 pKa = 11.84RR194 pKa = 11.84KK195 pKa = 10.5CSSIIVLLIVSMAWRR210 pKa = 11.84GNCTPARR217 pKa = 11.84LNKK220 pKa = 9.86FMGEE224 pKa = 3.91MDD226 pKa = 3.75VVAPGLGALIDD237 pKa = 3.92RR238 pKa = 11.84DD239 pKa = 3.88NIRR242 pKa = 11.84LVFSNFGPYY251 pKa = 8.72LTDD254 pKa = 3.62GNVEE258 pKa = 4.12EE259 pKa = 4.98VVNRR263 pKa = 11.84WMTFIPGSAVRR274 pKa = 11.84CRR276 pKa = 11.84VILQQSAGSGMTSLDD291 pKa = 3.73VIAKK295 pKa = 9.7AIHH298 pKa = 5.62EE299 pKa = 4.69HH300 pKa = 7.31PSFPWTNLGRR310 pKa = 11.84LYY312 pKa = 10.37PGEE315 pKa = 4.33WINALTALEE324 pKa = 4.04AVGANHH330 pKa = 7.51WYY332 pKa = 10.52GFRR335 pKa = 11.84SDD337 pKa = 4.16LGPVRR342 pKa = 11.84STQYY346 pKa = 11.36RR347 pKa = 11.84NISWVCKK354 pKa = 9.61EE355 pKa = 3.63LLIRR359 pKa = 11.84SGSDD363 pKa = 2.76PHH365 pKa = 6.93LQNKK369 pKa = 9.0AGWITTPKK377 pKa = 10.46NHH379 pKa = 6.23IQVEE383 pKa = 4.34GMIVDD388 pKa = 3.9HH389 pKa = 6.53LKK391 pKa = 11.01KK392 pKa = 10.75GDD394 pKa = 3.83TLVDD398 pKa = 4.2LVTPPTDD405 pKa = 3.34EE406 pKa = 4.26EE407 pKa = 4.21DD408 pKa = 4.55RR409 pKa = 11.84EE410 pKa = 4.44ITTLLQRR417 pKa = 11.84VSIYY421 pKa = 10.01PVNAGWTHH429 pKa = 5.38VPVAGRR435 pKa = 11.84GRR437 pKa = 11.84GRR439 pKa = 11.84QMGGAGGGGGQGGGGGGGGNQGGGGGGGGNQGGGGGGQGGQGGPGGQGRR488 pKa = 11.84GNGGGGSGQGGSSGPGAGSQSGAGGSGSGSSGAGGRR524 pKa = 11.84GRR526 pKa = 11.84GSGRR530 pKa = 11.84GRR532 pKa = 11.84GRR534 pKa = 11.84GRR536 pKa = 11.84GAPYY540 pKa = 10.6GGGEE544 pKa = 4.13GDD546 pKa = 4.11DD547 pKa = 4.51DD548 pKa = 3.96QVQVGAYY555 pKa = 9.26GGRR558 pKa = 11.84GDD560 pKa = 6.18DD561 pKa = 4.84DD562 pKa = 4.54DD563 pKa = 4.93QGGDD567 pKa = 3.56GDD569 pKa = 6.11DD570 pKa = 3.99NGEE573 pKa = 4.44DD574 pKa = 4.87DD575 pKa = 5.33SDD577 pKa = 4.46SSDD580 pKa = 3.47GNGGGVSDD588 pKa = 5.38ADD590 pKa = 4.2TIQEE594 pKa = 4.39GGDD597 pKa = 3.23

Molecular weight: 62.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KMY9|A0A1L3KMY9_9VIRU RNA-directed RNA polymerase L OS=Sanxia atyid shrimp virus 4 OX=1923358 PE=3 SV=1
MM1 pKa = 6.56MTSLPILLIAVLFGVATRR19 pKa = 11.84SASLQHH25 pKa = 6.79IIGYY29 pKa = 9.44DD30 pKa = 3.42CSGANAQIMKK40 pKa = 10.4LDD42 pKa = 3.57LTSVKK47 pKa = 10.02EE48 pKa = 4.09CRR50 pKa = 11.84DD51 pKa = 4.21DD52 pKa = 3.53IDD54 pKa = 4.08TVKK57 pKa = 9.42VTNKK61 pKa = 9.0IVQIIQHH68 pKa = 5.59KK69 pKa = 8.18TYY71 pKa = 10.79SPIHH75 pKa = 6.05IRR77 pKa = 11.84QCLLEE82 pKa = 3.94ITRR85 pKa = 11.84DD86 pKa = 3.02ITYY89 pKa = 10.04CGHH92 pKa = 7.25ASWLDD97 pKa = 3.56TSSTRR102 pKa = 11.84QYY104 pKa = 10.63KK105 pKa = 9.56VKK107 pKa = 10.19HH108 pKa = 5.32GHH110 pKa = 6.55LSYY113 pKa = 10.45IQPLGKK119 pKa = 9.4HH120 pKa = 5.32ACEE123 pKa = 4.9EE124 pKa = 3.88IHH126 pKa = 6.53KK127 pKa = 10.67SKK129 pKa = 10.76VFKK132 pKa = 10.7FQGKK136 pKa = 9.49LISHH140 pKa = 6.51SVTLNATEE148 pKa = 3.89THH150 pKa = 6.03AVVLSGSIDD159 pKa = 3.59SNGRR163 pKa = 11.84CKK165 pKa = 10.89NGGDD169 pKa = 3.63YY170 pKa = 11.08SDD172 pKa = 5.17AGTSVSNKK180 pKa = 9.38LVQGFLKK187 pKa = 9.9LTLSDD192 pKa = 4.2FYY194 pKa = 11.77GVLDD198 pKa = 4.95HH199 pKa = 7.84DD200 pKa = 4.47GQTLKK205 pKa = 9.99DD206 pKa = 3.6TKK208 pKa = 10.8HH209 pKa = 6.72GIDD212 pKa = 4.52CKK214 pKa = 10.4TSHH217 pKa = 6.34QYY219 pKa = 11.02CIDD222 pKa = 3.54TSIGEE227 pKa = 4.3IVWDD231 pKa = 3.74YY232 pKa = 10.66VQEE235 pKa = 4.28DD236 pKa = 4.04RR237 pKa = 11.84CHH239 pKa = 6.19KK240 pKa = 10.79NKK242 pKa = 9.9VDD244 pKa = 3.55VLYY247 pKa = 10.24TGSADD252 pKa = 3.51IISGLGTGEE261 pKa = 4.18SKK263 pKa = 10.38FVKK266 pKa = 9.36VTTGNTAFALEE277 pKa = 4.15ISGEE281 pKa = 4.14GWFCSKK287 pKa = 9.11PTYY290 pKa = 9.86STTQEE295 pKa = 4.12RR296 pKa = 11.84ITIIRR301 pKa = 11.84LEE303 pKa = 4.28YY304 pKa = 9.93IEE306 pKa = 4.83EE307 pKa = 4.12AMFVPSTILPMNVDD321 pKa = 3.88LTAYY325 pKa = 9.85INTKK329 pKa = 10.34FLLTQAAMGLQLTALYY345 pKa = 7.71KK346 pKa = 8.26TLKK349 pKa = 10.38RR350 pKa = 11.84GSCEE354 pKa = 3.46NRR356 pKa = 11.84KK357 pKa = 9.98LIFEE361 pKa = 4.13NRR363 pKa = 11.84IALARR368 pKa = 11.84LNPHH372 pKa = 5.85EE373 pKa = 4.17VVKK376 pKa = 10.74LVEE379 pKa = 4.54HH380 pKa = 6.75GEE382 pKa = 4.3GLFGRR387 pKa = 11.84VMGEE391 pKa = 3.63VMFITKK397 pKa = 9.54CVPILTTHH405 pKa = 7.49RR406 pKa = 11.84ATSQCYY412 pKa = 7.82QEE414 pKa = 4.83FPVVLKK420 pKa = 11.08DD421 pKa = 3.28NTTGYY426 pKa = 7.84LTPITRR432 pKa = 11.84ILTRR436 pKa = 11.84IGTQTTCSEE445 pKa = 3.73ILSPHH450 pKa = 5.48FHH452 pKa = 7.3IGDD455 pKa = 4.18FWYY458 pKa = 10.53SRR460 pKa = 11.84SPSVHH465 pKa = 4.7QQIPPITLDD474 pKa = 3.46PNLDD478 pKa = 3.57EE479 pKa = 5.53NEE481 pKa = 4.26WNFNMIPGLGHH492 pKa = 6.15SGLYY496 pKa = 9.77PEE498 pKa = 4.26EE499 pKa = 4.53AMRR502 pKa = 11.84EE503 pKa = 4.16MQRR506 pKa = 11.84EE507 pKa = 4.09MMFPASRR514 pKa = 11.84DD515 pKa = 3.65AVSSNMMSKK524 pKa = 10.41VVDD527 pKa = 3.54EE528 pKa = 5.36DD529 pKa = 3.99GNAIAYY535 pKa = 8.06HH536 pKa = 6.12HH537 pKa = 7.06LFNEE541 pKa = 4.27DD542 pKa = 5.12DD543 pKa = 3.89IDD545 pKa = 4.02TLTSSISGRR554 pKa = 11.84ISIFFKK560 pKa = 10.57WFGHH564 pKa = 5.28WASILVAVIIIIKK577 pKa = 10.23ISIALFDD584 pKa = 3.77IFINCLMIRR593 pKa = 11.84KK594 pKa = 9.3SGLSTKK600 pKa = 10.04FWIFGMFNSLTHH612 pKa = 6.63WILRR616 pKa = 11.84RR617 pKa = 11.84PSTSQSAVPLLPITSGPSNQTSTPPPSPVIIQMPSAPIYY656 pKa = 10.21PSVQPTQASIPRR668 pKa = 11.84RR669 pKa = 11.84TTPTVISRR677 pKa = 11.84PRR679 pKa = 11.84VLL681 pKa = 4.41

Molecular weight: 76.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3433

597

2155

1144.3

129.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.952 ± 0.682

1.573 ± 0.235

5.651 ± 0.333

5.156 ± 0.433

4.02 ± 0.291

7.282 ± 4.006

3.466 ± 0.475

7.166 ± 0.984

4.806 ± 0.916

10.457 ± 1.678

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.096

4.573 ± 0.365

4.369 ± 0.187

3.845 ± 0.009

5.214 ± 0.743

7.777 ± 0.442

6.088 ± 0.86

5.68 ± 0.347

1.515 ± 0.07

4.049 ± 1.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski