Synechococcus phage S-IOM18
Average proteome isoelectric point is 5.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 209 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R9TMT5|R9TMT5_9CAUD Uncharacterized protein OS=Synechococcus phage S-IOM18 OX=754039 GN=SWYG_00028 PE=4 SV=1
MM1 pKa = 7.43 PQFTLICTDD10 pKa = 3.25 EE11 pKa = 5.05 DD12 pKa = 4.05 STVTTKK18 pKa = 10.68 EE19 pKa = 3.89 FEE21 pKa = 4.19 ATVLDD26 pKa = 4.1 DD27 pKa = 5.55 VVDD30 pKa = 3.75 KK31 pKa = 10.24 TQDD34 pKa = 3.19 FLKK37 pKa = 10.98 GVGYY41 pKa = 10.38 CFEE44 pKa = 4.24 EE45 pKa = 4.61 LHH47 pKa = 5.63 TQVYY51 pKa = 8.4 PLPEE55 pKa = 5.11 SNDD58 pKa = 3.51 DD59 pKa = 4.67 DD60 pKa = 4.7 YY61 pKa = 12.26 SSLYY65 pKa = 10.44 KK66 pKa = 10.88 DD67 pKa = 3.21 VDD69 pKa = 3.34
Molecular weight: 7.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.706
IPC_protein 3.668
Toseland 3.452
ProMoST 3.783
Dawson 3.668
Bjellqvist 3.897
Wikipedia 3.643
Rodwell 3.503
Grimsley 3.376
Solomon 3.643
Lehninger 3.605
Nozaki 3.808
DTASelect 4.037
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.795
Patrickios 0.769
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.767
Protein with the highest isoelectric point:
>tr|R9TLD6|R9TLD6_9CAUD Terminase small subunit OS=Synechococcus phage S-IOM18 OX=754039 GN=SWYG_00076 PE=4 SV=1
MM1 pKa = 7.55 AARR4 pKa = 11.84 SKK6 pKa = 10.86 SLSGGKK12 pKa = 10.03 LIEE15 pKa = 4.34 TKK17 pKa = 9.98 PKK19 pKa = 9.15 KK20 pKa = 8.43 TRR22 pKa = 11.84 QGAGQHH28 pKa = 4.98 TKK30 pKa = 10.52 YY31 pKa = 10.77 AATSRR36 pKa = 11.84 NNAKK40 pKa = 9.86 KK41 pKa = 10.15 RR42 pKa = 11.84 YY43 pKa = 8.5 RR44 pKa = 11.84 GQGRR48 pKa = 3.34
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.178
IPC2_protein 10.57
IPC_protein 11.637
Toseland 11.813
ProMoST 12.252
Dawson 11.828
Bjellqvist 11.754
Wikipedia 12.237
Rodwell 11.901
Grimsley 11.857
Solomon 12.252
Lehninger 12.164
Nozaki 11.798
DTASelect 11.754
Thurlkill 11.798
EMBOSS 12.281
Sillero 11.798
Patrickios 11.637
IPC_peptide 12.252
IPC2_peptide 11.199
IPC2.peptide.svr19 8.823
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
209
0
209
52984
45
6387
253.5
28.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.172 ± 0.242
0.87 ± 0.106
6.636 ± 0.166
6.3 ± 0.322
4.279 ± 0.114
8.016 ± 0.376
1.483 ± 0.116
6.032 ± 0.228
5.609 ± 0.426
7.159 ± 0.187
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.167 ± 0.231
5.639 ± 0.183
3.896 ± 0.153
3.771 ± 0.116
4.054 ± 0.179
6.983 ± 0.244
7.823 ± 0.519
6.81 ± 0.211
1.098 ± 0.089
4.203 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here